anti-Rab1-GTP mAb (rec.) (ROF7)

AdipoGen Life Sciences
Product Code: AG-27B-0006
Product Group: Primary Antibodies

CodeSizePrice
AG-27B-0006-C100100 ug£290.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Human
Antibody Isotype: IgG2λ
Antibody Clonality: Recombinant Antibody
Antibody Clone: ROF7
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunoprecipitation (IP)
Shipping:
-20°C
Storage:
-20°C

Images

1 / 1
Rab1-GTP is detected by immunocytochemistry using anti-Rab1-GTP, mAb (ROF7) (Prod. No.  AG-27B-0006). Method: HeLa cells are grown in standard culture conditions, fixed with paraformaldehyde (3%), permeablized in  PBS+ BSA 0.2

Rab1-GTP is detected by immunocytochemistry using anti-Rab1-GTP, mAb (ROF7) (Prod. No.  AG-27B-0006). Method: HeLa cells are grown in standard culture conditions, fixed with paraformaldehyde (3%), permeablized in  PBS+ BSA 0.2

Further Information

Alternate Names/Synonyms:
Ras-related Protein Rab-1
Concentration:
1mg/ml
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
Immunogen:
Full length canine Rab1.
Long Description:
Recombinant Antibody. Recognizes human, mouse, rat and dog Rab1a-GTP and Rab1b-GTP. Species cross-reactivity: Dog, Human, Mouse, Rat. Clone: ROF7. Isotype: Human IgG2lambda. Applications: ICC, IP. Host: Purified from HEK 293 cell culture supernatant. Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. Rab1 (Ypt1 in yeast) is a small GTPase that plays a well-established role in mediating ER-to-Golgi protein transport in both yeast and mammalian cells. Rab1 recruits effector proteins to budding COPII vesicles at the ER, forming cis-SNARE complexes that promote targeting to and fusion of these vesicles with the cis-Golgi. Rab1 is also involved in COPI vesicle formation and other distinct transport pathways, including ER-to-Golgi intermediate compartment (ERGIC)-to-cell periphery trafficking. Recently Rab1 has been shown to function in antimicrobial autophagy (autophagosome formation), as well as other forms of autophagy in mammalian cells in a way independent of ER-to-Golgi trafficking. anti-Rab1-GTP, monoclonal antibody (recombinant) (ROF7) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
NCBI, Uniprot Number:
P62822
Other data:
anti-Rab1-GTP, monoclonal antibody (recombinant) (ROF7) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.
Package Type:
Plastic Vial
Product Description:
Rab1 (Ypt1 in yeast) is a small GTPase that plays a well-established role in mediating ER-to-Golgi protein transport in both yeast and mammalian cells. Rab1 recruits effector proteins to budding COPII vesicles at the ER, forming cis-SNARE complexes that promote targeting to and fusion of these vesicles with the cis-Golgi. Rab1 is also involved in COPI vesicle formation and other distinct transport pathways, including ER-to-Golgi intermediate compartment (ERGIC)-to-cell periphery trafficking. Recently Rab1 has been shown to function in antimicrobial autophagy (autophagosome formation), as well as other forms of autophagy in mammalian cells in a way independent of ER-to-Golgi trafficking. anti-Rab1-GTP, monoclonal antibody (recombinant) (ROF7) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
Purity:
>95% (SDS-PAGE)
Source / Host:
Produced without the use of animals. Purified from HEK 293 cell culture supernatant.
Specificity:
Recognizes human, mouse, rat and dog Rab1a-GTP and Rab1b-GTP.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

References

Characterization of single chain antibody targets through yeast two hybrid: O. Vielemeyer, et al.; BMC Biotechnol. 10, 59 (2010)