anti-beta-Tubulin mAb (rec.) (S11B)

AdipoGen Life Sciences
Product Code: AG-27B-0008
Product Group: Primary Antibodies

CodeSizePrice
AG-27B-0008-C100100 ug£280.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Human
Antibody Isotype: IgG2λ
Antibody Clonality: Recombinant Antibody
Antibody Clone: S11B
Regulatory Status: RUO
Target Species:
  • Drosophila (fruit fly)
  • Human
  • Monkey
  • Mouse
  • Porcine (pig)
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Western Blot (WB)
Shipping:
-20°C
Storage:
-20°C

Images

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Human beta-Tubulin is detected by immunocytochemistry using anti-beta-Tubulin, mAb (rec.) (S11B) (Prod. No. AG-27B-0008). Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-

Human beta-Tubulin is detected by immunocytochemistry using anti-beta-Tubulin, mAb (rec.) (S11B) (Prod. No. AG-27B-0008). Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-

Further Information

Alternate Names/Synonyms:
Tubulin beta Chain, Tubulin beta-5 chain
Concentration:
1mg/ml
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
Immunogen:
Full length tubulin from pig brain.
Long Description:
Recombinant Antibody. Recognizes human, mouse, rat, pig, drosophila and monkey beta-tubulin. Species cross-reactivity: Drosophila, Human, Monkey, Mouse, Pig, Rat. Clone: S11B. Isotype: Human IgG2lambda. Applications: ELISA, ICC, WB. Host: Purified from HEK 293 cell culture supernatant. Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. anti-beta-Tubulin, monoclonal antibody (recombinant)(S11B) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
NCBI, Uniprot Number:
N/A
Other data:
anti-beta-Tubulin, monoclonal antibody (recombinant) (S11B) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.
Package Type:
Plastic Vial
Product Description:
anti-beta-Tubulin, monoclonal antibody (recombinant)(S11B) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
Purity:
>95% (SDS-PAGE)
Source / Host:
Produced without the use of animals. Purified from HEK 293 cell culture supernatant.
Specificity:
Recognizes human, mouse, rat, pig, drosophila and monkey beta-tubulin.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

References

Recombinant antibodies selected against subcellular fractions to track endogenous protein dynamics in vivo: C. Nizak, et al.; Traffic 7, 739 (2003)