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| Type | Category | Supplier | ||
|---|---|---|---|---|
 | Some researchers require a product which is serum-free and has had no contact with human or animal cells. We have, therefore, designed BriClone-SF which has the same basal medium and biological activities as BriClone but has no added serum or other substances of human or animal origin and has not been exposed to cells. BriClone-SF contains added recombinant human proteins produced in bacterial or yeast host cells. | |||
Documents | Cell Culture | NICB | ||
 | Protocol for Use for BriClone-SF Hybridoma Cloning Supplement | |||
Documents | Cell Culture | NICB | ||
 | Material Safety Data Sheet for BriClone-SF Hybridoma Cloning Supplement | |||
Documents | Cell Culture | NICB | ||
 | BriClone is added to Hybridoma culture media as a 5% v/v supplement to replace the time-consuming task of preparing feeder cells during routine cloning of hybridomas. | |||
Documents | Cell Culture | NICB | ||
 | BriClone has been cited in many publications over the years, and in a range of research areas. | |||
Documents | Cell Culture | NICB | ||
 | NICB has over 25 years of experience in antibody development & developed BriClone, a supplement for improving fusion efficiency and supporting cloning outgrowth of hybridoma cells at those fragile early steps. | |||
Documents | Cell Culture | NICB | ||
 | In 2005, researchers in Graduate School of Dentistry and Dept. of Oral Pathology at Osaka Dental University (Japan) were seeking to develop novel agents for use in periodontal regeneration therapy. The researchers published two studies utilising hybridoma technology to successfully produce monoclonal antibodies (mAbs) against the rat periodontium (RPT1) and the human cemento-periodontal ligament junction (anti-TAP). | |||
Documents | Cell Culture | NICB | ||
 | An overview of the research applications of BriClone Hybridoma Cloning and Selection Supplement. | |||
Documents | Cell Culture | NICB | ||
 | BriClone has been manufactured onsite for over 25 years by research scientists at the publicly-funded National Institute for Cellular Biotechnology, (Dublin City University, Dublin, Ireland) and has a long history of application in research internationally. | |||
Documents | Cell Culture | NICB | ||
 | BriClone is an additive for the cloning medium used in the post-fusion stages of hybridoma production and for improving the efficiency of hybridoma cells cloning.
To demonstrate the stability of BriClone, various simulated conditions were assessed mimicking different temperature exposures.
Post-fusion hybridoma production was assessed using BriClone under different conditions (overnight at room temperature) as a positive control, as well as one week stored at 4°C. | |||
Documents | Cell Culture | NICB | ||
 | BriClone is an additive for the cloning medium used in the post-fusion stages of hybridoma production and for improving the efficiency of hybridoma cells cloning.
Many commercial additives are available for improving the improving the efficiency of cell cloning at the post-fusion stages of hybridoma production. In the following experiment, BriClone was compared to two competitors as a supplement for post-fusion hybridoma production by assessing fusion efficiency. | |||
Documents | Cell Culture | NICB | ||
 | BriClone is an additive for the cloning medium used in the post-fusion stages of hybridoma production and for improving the efficiency of hybridoma cells cloning.
The use of feeder cells to support the outgrowth of hybridomas has many disadvantages, like batch-to-batch variation of the feeder cells and competition between feeder cells and freshly-fused hybridomas for nutrients in the culture medium. BriClone has been specially developed to overcome these common problems in the post-fusion period. | |||
Documents | Cell Culture | NICB | ||
 | Protocol for Use for BriClone Hybridoma Cloning Supplement | |||
Documents | Cell Culture | NICB | ||
 | Material Safety Data Sheet for BriClone Hybridoma Cloning Supplement | |||
Documents | Cell Culture | NICB | ||
 | BriClone is added to Hybridoma culture media as a 5% v/v supplement to replace the time-consuming task of preparing feeder cells during routine cloning of hybridomas. | |||
Documents | Cell Culture | NICB | ||