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| In competitive ELISA, unlabeled antibody is incubated in the presence of its antigen. Then these bound antibody/antigen complexes are then added to an antigen coated well. After washing, unbound antibodies are removed. The more analytes in the sample, the less antibodies will be able to bind to antigens in the well. The signal is then detected using labeled secondary antibodies and the decrease in signal is compared to a control. The major advantage of a competitive ELISA is the ability to use crude or impure samples and still selectively bind any antigen that may be present. |
Videos | Methods | Abnova Corporation | |
| Immunofluorescence is a technique to visualize a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye. We use the indirect immunofluorescence staining to perform cells fixed on slides and examine under a fluorescence microscope. |
Videos | Methods | Abnova Corporation | |
| Cell culture is the process by which cells are grown under controlled conditions. This video show you how to culture attached cells from cell thrawing, maintain, to cell freezing. |
Videos | Methods | Abnova Corporation | |
| Polymerase Chain Reaction (PCR) is a technique to amplify few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. There are three major steps (Denaturation, Annealing and Extension) in a PCR and repeat for 30 or 40 cycles |
Videos | Methods | Abnova Corporation | |
| The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample. In the assay, the antigen of interested is immobilized by direct adsorption to the assay plate. Detection of the antigen can then be performed by using a matched set of primary antibody and conjugated secondary antibodies. |
Videos | Methods | Abnova Corporation | |
| Immunohistochemistry is a method of detecting the presence of specific proteins in cells or tissues. It is widely used in basic research to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue. |
Videos | Methods | Abnova Corporation | |
| Reverse transcription PCR includes two steps. The first step is reverse transcription, in which RNA is reverse transcribed to its DNA complement (complementary DNA, or cDNA) using reverse transcriptase and primers. The second step is amplification using traditional or real-time PCR. |
Videos | Methods | Abnova Corporation | |
| Real-time PCR, also called quantitative real time PCR (Q-PCR/qPCR), is used to amplify and simultaneously quantify a targeted DNA molecule. It enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of one or more specific sequences in a DNA sample. |
Videos | Methods | Abnova Corporation | |
| MBL International Corporation offers a line of highly specific antibodies conjugated to magnetic beads. This method is an advantage over conventional immunoprecipitation of tagged proteins because it eliminates time consuming centrifugation steps. The magnetic beads are covalently bound to a Tag antibody and the beads are easily isolated using a magnetic rack. |
Videos | Immunology | MBL | |
| Oxidative Stress Markers | Oxidative DNA Damage Markers | Protein Oxidation Markers | Lipid Peroxidation Markers | Antioxidants Detection | Specific Oxidative Stress Marker Antibodies |
Documents | Antibody Tools | JaICA | |
| Nordic-MUbio oers the highest quality range of products for the study and analysis of human immunoglobulins |
Documents | Immunology | Nordic MuBio | |
| Educational video for the RIP-Chip assay technology for microRNA. Identify miRNAs bound to their mRNA targets. |
Videos | Methods | MBL | |
| MBL has incorporated the patented mutation of the a3 domain in their HLA class I Tetramers and is the only manufacturer of MHC multimers with this technology to enhance specificity for the antigen-specific T cell receptor. |
Videos | Immunology | MBL | |
| Primary culture of chick embryo myocyte culture can be used for the detection and assessment of drug responses on cardiomyocyte. |
Videos | Primary Cells | Abnova Corporation | |