qPCR can be a powerful tool for analyzing gene expression. When designed properly, however its strength relies on the quality of the primers used in it. Our scientists know this and follow stringent guidelines to design primers for our GeneQuery qPCR arrays. When researchers design their own primers without comprehensive knowledge of good primer design, qPCR results can be inadvertently influenced yielding unreliable and inconsistent data. Our webinar will cover guidelines used by our scientists in designing our quality GeneQuery qPCR primers arrays while highlighting our human endothelial cell biology array. An extended FAQ session for your questions and concerns will follow.
Objectives:
Delineate frequently overlooked primer optimization parameters during qPCR design.
Demonstrate the benefits of properly optimized primers in qPCR analysis.
Exemplify the ease and use of GeneQuery qPCR arrays in gene expression analysis.
Recently, Netrin-1 and its receptors DCC (Deleted in Colorectal Carcinoma)or UNC5b, described for their respective survival/death functions in normal and oncogenic contexts, has been identified in a search for soluble regulators/modulators of somatic cell reprogramming to pluripotency
CSF samples were analysed within 2-6 hours of sampling or after 72 hours of storage at +4°C in Transfix.Transfix preserved light scatter and key antigen expression patterns to allow for analysis of diagnostic and follow up CSF specimens for patients with CSN infiltration.
This brochure brings together a comprehensive list of products for use in clinical flow cytometry, including antibodies, isoptype controls, reagents, software, instruments and diagnostic kits.
Signosis provides several different tools to facilitate the analysis of transcription factors. The multifunctional products are designed to meet different needs, and contribute to exciting research results.
Multiple transcription factors (TFs) have been reported to associate with stem cell self-renewal and pluripotency such as EGR1, OCT4, FOXD3, FOXO, Nanog, SOX2, SOX18, ETS, GLI, KLF4, MEF2, Myc, RNUX1,
Pax6, TCF/LEF and GATA. Analyzing the activities of these TFs can provide valuable insight into the study of stem cells. Signosis has developed Stem Cell Transcription Factor Activation Plate Array to analyze the activities of 16 stem cell related TFs simultaneously for mammalian samples. The assay can be used with the whole cell lysate from 1000-10000 cells.
Signosis has developed a sensitive single cell RT-PCR directly cell lysates without RNA preparation. Therefore, a small number of cells is enough for RT-PCR analysis. Signosis now offers the optimized
buffers for subsequent RT-PCR, which followed cell lysis with CL™ cell lysis buffer. All of reagents for cDNA synthesis and PCR amplification in addition to CL™ cell lysis buffer are included in the kit.
Facilitate the determination of the optimal cell density, time point of analysis and multiplexing of readouts
(e.g. cytokine(s) ELISA) of TLR4 specific cellular activation in a highly reproducible and safe procedure.