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Immunoassays Services

  • AllCells specialises in Immunoassays using haematopoietic and immunological cells collected from healthy or diseased donors.
  • Their onsite collection for Bone Marrow, Whole Blood and Leukapheresis products allows direct analysis within 1 hour of cell collection.
  • Choose samples for analysis by blood type, gender, ethnicity and age.
  • Donor recruitment programmes are available plus donor recall and matched samples.

Allcells offer a number of Immunoassay services including:

ELISA: Enzyme-linked immunosorbent assay

ELISA is a quantitative assay that can be used to characterize expression levels of soluble proteins. This assay features a high throughput, scalable, easily-read platform. Due to its flexibility, ELISA can be applied to numerous targets depending on your specific requirements.

Case Study:

Anti-CD40-induced IgG secretion from plasma cells of 3 healthy donors.

Immunoassays

Figure A. Plasma B cells isolated from peripheral blood of 3 human donors were stimulated with (blue bars) or without (red bars, undetectable) anti-CD40. Supernatants were assayed for lgG by ELISA. B. Standard curve showing range of IgG protein detection.

ELISpot

Enzyme-linked immunospot assay, or ELISpot, is a colorimetric assay which enables quantitative, single cell analysis of protein expression levels. Due to its high sensitivity and reproducibility, the ELISpot is an industry standard in antigenic response in the form of cytokine secretion.

Immunoassay Screening

Labelling of T cells with the fluorescent dye 5,6-carboxyfluorescein diacetate succinimidyl ester (CFSE) allows for quantitative phenotyping of T cell division in response to antigen or other stimuli by flow cytometric methods. T cell proliferation assays are essential for peptide epitope mapping of na´ve T cells and for characterizing full protein immunogenicity in DC-T cell assays.

Immunoassay

Proliferation of CD4+ and CD8+ primary human T cells

Figure. CD4+ (A) and CD8+ (B) T cells were isolated from peripheral human blood and labelled with 5,6-carboxyfluorescein diacetate succinimidyl ester (CFSE). Labelled cells were cultured 7d with or without a stimulatory antibody cocktail. Fluorescence was measured by flow cytometry.

Luminex (single, multiplex)

The Luminex Bead Array System allows for a high throughput, multi-target analysis of cytokines, proteins, and nucleic acids for researchers. The system is important for many clinical and research applications.

Human Donor HLA Typing

HLA (Human Leukocyte Antigen) Typing of donors is an important aspect of research being conducted within inflammatory, immune responses pertaining to antigen presentation. AllCells Bioservices provides HLA Typing of our donors for specific A2, DRB4 loci. We offer this service based on the specific research criteria you may have. Please inquire with us today about our HLA Typing capabilities and how we may assist with your project needs.

For further information about the Immunoassay services that AllCells can provide, or if you require a quotation, please contact office@caltagmedsystems.co.uk or call +44 (0)1280 827460.

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