CycLex® Aurora A Kinase Assay / Inhibitor Screening Kit
The CycLex® Aurora A Kinase Assay / Inhibitor Screening Kit uses recombinant Lats2 as a specific Aurora A substrate. A detector antibody specifically recognises only the phosphorylated form of the serine83 residue on Lats2. The Aurora A Assay Kit is suitable for assaying the kinetics of purified or partially purified Aurora-A as well as for screening Aurora-A inhibitors.
Fig. 1 Dose dependency of recombinant Aurora-A enzyme reaction, as measured using the CycLex® Aurora A Kinase Assay / Inhibitor Screening kit.
Product Information
CycLex® Aurora A kinase Assay / Inhibitor Screening Kit (96 wells)
Further Information
Aurora kinases regulate centrosome maturation, chromosome segregation, and cytokinesis. A-type Aurora kinases localize to both centrosomes and spindle microtubules and have been implicated in spindle assembly. The B-type Aurora kinases are present at centromeres in prophase and metaphase before they relocalize to the central spindle and the mid-body in anaphase and telophase. The C-type Aurora kinases are expressed primarily in the testis and some tumour cell lines, where they have been localized to spindle poles. All three Aurora kinases family members have been reported to be over-expressed in many human cancers, and elevated expression has been correlated with chromosomal instability, and in some instances with clinically aggressive disease).
Related Products
Aurora C Positive Control
Anti-PhosphoLats2 (Ser83) Monoclonal Antibody
Caltag Medsystems is the distributor of MBLI’s products in the UK and Ireland. If you have any questions about these products, please contact us.
To view MBLI’s catalogue of Kinase Assay Kits, please click here