{"id":1608,"date":"2016-08-25T15:56:30","date_gmt":"2016-08-25T15:56:30","guid":{"rendered":"http:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/steps-for-ChIP.jpg"},"modified":"2016-08-25T15:58:35","modified_gmt":"2016-08-25T15:58:35","slug":"steps-for-chip","status":"inherit","type":"attachment","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/steps-for-chip\/","title":{"rendered":"steps-for-ChIP"},"author":13,"featured_media":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"class_list":["post-1608","attachment","type-attachment","status-inherit","hentry"],"description":{"rendered":"<p class=\"attachment\"><a href='https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/steps-for-ChIP.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"209\" height=\"300\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/steps-for-ChIP-209x300.jpg\" class=\"attachment-medium size-medium\" alt=\"Basic ChIP Steps: Crosslink to bind proteins to DNA. Isolate and shear DNA. Immunoprecipitate with protein-specific antibody. Crosslink reversal and DNA clean-up. Quantitate the DNA.\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/steps-for-ChIP-209x300.jpg 209w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/steps-for-ChIP.jpg 300w\" sizes=\"auto, (max-width: 209px) 100vw, 209px\" \/><\/a><\/p>\n<p>the overarching standard ChIP (X-ChIP) procedure<\/p>\n"},"caption":{"rendered":"<p>the overarching standard ChIP (X-ChIP) procedure<\/p>\n"},"alt_text":"Basic ChIP Steps: Crosslink to bind proteins to DNA. Isolate and shear DNA. Immunoprecipitate with protein-specific antibody. Crosslink reversal and DNA clean-up. Quantitate the DNA.","media_type":"image","mime_type":"image\/jpeg","media_details":{"width":300,"height":430,"file":"steps-for-ChIP.jpg","sizes":{"thumbnail":{"file":"steps-for-ChIP-150x150.jpg","width":150,"height":150,"mime_type":"image\/jpeg","source_url":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/steps-for-ChIP-150x150.jpg"},"medium":{"file":"steps-for-ChIP-209x300.jpg","width":209,"height":300,"mime_type":"image\/jpeg","source_url":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/steps-for-ChIP-209x300.jpg"},"featured-blog-large":{"file":"steps-for-ChIP-300x350.jpg","width":300,"height":350,"mime_type":"image\/jpeg","source_url":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/steps-for-ChIP-300x350.jpg"},"featured-blog-medium":{"file":"steps-for-ChIP-270x270.jpg","width":270,"height":270,"mime_type":"image\/jpeg","source_url":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/steps-for-ChIP-270x270.jpg"},"featured":{"file":"steps-for-ChIP-300x300.jpg","width":300,"height":300,"mime_type":"image\/jpeg","source_url":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/steps-for-ChIP-300x300.jpg"},"featured-blog-medium-small":{"file":"steps-for-ChIP-230x230.jpg","width":230,"height":230,"mime_type":"image\/jpeg","source_url":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/steps-for-ChIP-230x230.jpg"},"full":{"file":"steps-for-ChIP.jpg","width":300,"height":430,"mime_type":"image\/jpeg","source_url":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/steps-for-ChIP.jpg"}},"image_meta":{"aperture":0,"credit":"","camera":"","caption":"","created_timestamp":0,"copyright":"","focal_length":0,"iso":0,"shutter_speed":0,"title":"","orientation":0,"keywords":[]}},"post":null,"source_url":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/steps-for-ChIP.jpg","filename":"steps-for-ChIP.jpg","filesize":25484,"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media\/1608","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/attachment"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=1608"}]}}