{"id":10401,"date":"2024-06-11T14:41:43","date_gmt":"2024-06-11T14:41:43","guid":{"rendered":"https:\/\/www.caltagmedsystems.co.uk\/information\/?p=10401"},"modified":"2024-06-11T14:41:44","modified_gmt":"2024-06-11T14:41:44","slug":"fresh-human-animal-hepatocytes","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/fresh-human-animal-hepatocytes\/","title":{"rendered":"Fresh Human &amp; Animal Hepatocytes"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\"><strong>Fresh Plated Hepatocytes<\/strong><\/h2>\n\n\n\n<h4 class=\"wp-block-heading\">Key Features of Human &amp; Animal Fresh-Plated Hepatocytes:<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Multi-format plated cells and custom preparation are available<\/li>\n\n\n\n<li>Greater viability and confluence of cells in monolayer<\/li>\n\n\n\n<li>High expression levels of major hepatic drug-metabolising enzymes, co-factors and cell membrane receptors<\/li>\n\n\n\n<li>Greater flexibility for applications with either short or long-term cultures for human hepatocytes and cells derived from multiple animal species, either single or pooled donors<\/li>\n\n\n\n<li>Full characterisation data available for each product batch sheet<\/li>\n\n\n\n<li>Certificate of Analysis available for each batch<\/li>\n\n\n\n<li>General patient information (age, gender, race, health conditions, serology)<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>Fresh-Plated Human Hepatocytes<\/strong><\/h3>\n\n\n\n<p>To provide adherent monolayer cultures, Biopredic plates freshly isolated and viable cells, at a desired seeding density, onto collagen-coated culture supports according to the customer\u2019s request\/ordering.<\/p>\n\n\n\n<p>After overnight attachment, the seeding medium is replaced with either a short-term culture medium or long-term culture medium (<em>Lanford et al., 1989<\/em>) to produce short-term (\u2264 3 days) or long-term hepatocytes (\u2264 6 days) cultures in which cells retain a differentiated phenotype for at least 1 week.<\/p>\n\n\n\n<p>Both freshly prepared short-term and long-term cultured hepatocytes contain all major phase I and phase II enzymes as well as the whole gene regulation pathway necessary to investigate the potentiality of drugs to induce phase I and phase II enzymes.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Characteristics for Fresh-Plated Human Hepatocytes<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Cell viability and cell culture period after isolation and seeding<\/li>\n\n\n\n<li>Cell morphology and culture confluence by microphotography<\/li>\n\n\n\n<li>Phase I drug-metabolising enzymes with a cocktail of probe substrates: CYP1A2\/ 3A4\/ 2B6\/ 2D6\/ 2C19<\/li>\n\n\n\n<li>Phase II drug-metabolising enzymes:&nbsp;UGT1A1\/ UGT1A6\/ UGT1A9\/ UGT2B15\/ SULT1A1\/ SULT1A3\/ 4\/ SULT1E1<\/li>\n\n\n\n<li>Serological and sterility control data<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>Fresh-Plated Animal Hepatocytes<\/strong><\/h3>\n\n\n\n<p>To provide plated animal hepatocyte cultures, the freshly isolated and viable cells are plated at a desired seeding density onto a collagen-coated culture surface according to the customer\u2019s request\/order.<\/p>\n\n\n\n<p>After an appropriate period of cell attachment (from 4 hours to overnight depending upon the species), the seeding medium is replaced with a short-term culture medium and then packaged for shipment.<\/p>\n\n\n\n<p>Fresh-plated cultured hepatocytes contain all major phase I and phase II enzymes and can be used in a wide range of in-vitro applications.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Characteristics for Fresh-Plated Human Hepatocytes<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Donor animal information (species, identification number, age, sex, serology)<\/li>\n\n\n\n<li>Viability, yield, plateability, and morphology before and after shipment<\/li>\n\n\n\n<li>Phase I drug-metabolising enzymes with either a cocktail of probe substrates or a specific probe substrate:\u00a0<em>CYP1A\/ CYP2D\/ CYP3A<\/em><\/li>\n\n\n\n<li>Phase II drug-metabolising enzymes:\u00a0<em>UGT1A\/ UGT2B\/ SULT1A\/SULT1E.\u00a0<\/em><\/li>\n\n\n\n<li>Glucuronidation and sulfation activity of acetaminophen<\/li>\n\n\n\n<li>Sterility control data<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Fresh Hepatocytes in Suspension<\/strong><\/h2>\n\n\n\n<p>In order to provide suspended human &amp; animal hepatocytes, freshly isolated hepatocytes are rapidly suspended and preserved in Biopredic\u2019s optimised hypothermic medium to maintain cell viability and functional activity during the shipment period (up to 48 hours).<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>Fresh Human Hepatocytes in Suspension<\/strong><\/h3>\n\n\n\n<h4 class=\"wp-block-heading\">Key Features of Fresh Human Hepatocytes in Suspension:<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Good availability of fresh cells with a proprietary production process<\/li>\n\n\n\n<li>High-quality products combined with optimised hypothermic shipping conditions<\/li>\n\n\n\n<li>Stable and retained viability and expression level of metabolising enzymes<\/li>\n\n\n\n<li>Greater flexibility to handle cells either in suspension or plated cell culture<\/li>\n\n\n\n<li>Characterisation data available for each product batch sheet\n<ul class=\"wp-block-list\">\n<li>Viability after isolation and after the cold shipping period, yield &amp; plateability after the cold storage period, morphology before and after shipment<\/li>\n\n\n\n<li>Phase I drug-metabolising enzymes with a cocktail of probe substrates: CYP1A2\/ 3A4\/ 2B6\/ 2D6\/ 2C19<\/li>\n\n\n\n<li>Phase II drug-metabolising enzymes: UGT1A1\/ UGT1A6\/ UGT1A9\/ UGT2B15\/ SULT1A1\/ SULT1A3\/ 4\/ SULT1E1<\/li>\n\n\n\n<li>Serological and sterility control data<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li>General patient information (age, gender, race, health conditions, serology)<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>Fresh Animal Hepatocytes in Suspension<\/strong><\/h3>\n\n\n\n<h4 class=\"wp-block-heading\">Key Features of Fresh Animal Hepatocytes in Suspension:<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Good availability of fresh cells from multiple species thanks to our scheduled production<\/li>\n\n\n\n<li>Custom preparation are available for both single-donor and pooled cells<\/li>\n\n\n\n<li>Large pooled lot size allows for conducting multiple experiments from the same pool of donors.<\/li>\n\n\n\n<li>High-quality product combined with optimised hypothermic shipping conditions<\/li>\n\n\n\n<li>Stable and retained viability and expression level of metabolizing enzymes,<\/li>\n\n\n\n<li>Greater flexibility to handle cells either in suspension or plated cell culture<\/li>\n\n\n\n<li>Certificate of Analysis available for each batch<\/li>\n\n\n\n<li>Characterisation data available for each product batch sheet\n<ul class=\"wp-block-list\">\n<li>Donor animal information (species, identification number, age, sex, serology)<\/li>\n\n\n\n<li>Viability, yield, plateability, and morphology before and after shipment<\/li>\n\n\n\n<li>Phase I drug-metabolising enzymes with either a cocktail of probe substrates or a specific probe substrate:\u00a0<em>CYP1A\/ CYP2D\/ CYP3A<\/em><\/li>\n\n\n\n<li>Phase II drug-metabolising enzymes:\u00a0<em>UGT1A\/ UGT2B\/ SULT1A\/SULT1E.\u00a0<\/em><\/li>\n\n\n\n<li>Glucuronidation and sulfation activity of acetaminophen<\/li>\n\n\n\n<li>Sterility control data<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-alpha-channel-opacity has-background\" style=\"background-color:#21318f;color:#21318f\"\/>\n\n\n\n<p><a href=\"https:\/\/www.caltagmedsystems.co.uk\/\">Caltag Medsystems\u00a0<\/a>is the distributor of\u00a0<a href=\"https:\/\/www.caltagmedsystems.co.uk\/biopredic\/\">Biopredic International<\/a>\u00a0products in the UK and Europe. If you have any questions about these products, please\u00a0<a href=\"https:\/\/www.caltagmedsystems.co.uk\/contact.php\">contact us<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Biopredic International provides both human and animal hepatocytes, either plated or in suspension.<\/p>\n","protected":false},"author":13,"featured_media":10412,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1,749],"tags":[902,633,798,884],"class_list":["post-10401","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-general-information","category-hepatocytes","tag-animal-hepatocytes","tag-biopredic","tag-fresh","tag-human-hepatocytes"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/10401","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=10401"}],"version-history":[{"count":10,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/10401\/revisions"}],"predecessor-version":[{"id":10413,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/10401\/revisions\/10413"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media\/10412"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=10401"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=10401"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=10401"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}