{"id":1076,"date":"2024-09-16T10:10:09","date_gmt":"2024-09-16T10:10:09","guid":{"rendered":"http:\/\/www.caltagmedsystems.co.uk\/information\/?p=1076"},"modified":"2025-04-25T15:31:04","modified_gmt":"2025-04-25T15:31:04","slug":"keima-red-for-mitophagy-detection-in-living-cells","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/keima-red-for-mitophagy-detection-in-living-cells\/","title":{"rendered":"Keima-Red for Mitophagy Detection in Living Cells"},"content":{"rendered":"\n

CoralHue\u00ae<\/sup> monomeric and dimeric Keima-Red are red fluorescent proteins with the largest commercially available Stokes shift (ex. 440 nm, em. 620 nm) making Keima-Red a superb reporter protein for multicolour fluorescence analysis. Because of this characteristic, they are excited by a very short wavelength but emit a long wavelength. Keima is named after a shogi (Japanese chess) piece Keima that can move in a hopping manner, similar to the knight in the game of chess.<\/p>\n\n\n\n

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\"Keima_3\"<\/figure>\n\n\n\n

The Keima-Red protein cloning plasmids allow for the insertion of cDNA sequences to create protein fusion products between the protein of interest and Keima. Keima-Red cloning plasmids create protein fusion products that are useful for tracking protein localisation within cells as well as monitoring gene expression. Keima-Red is also available as target-specific constructs which allow for Keima-Red protein fusion products to be directed to either the mitochondria or the plasma membrane.<\/p>\n\n\n\n

Performance and Use<\/h2>\n\n\n\n

Labelling Organelles<\/h3>\n\n\n\n
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dKeima-Red and mKeima-Red are easily expressed in a wide range of organisms. dKeima-Red and mKeima-Red have been demonstrated in the nucleoplasm, plasma membrane, and mitochondria targeting signal models.<\/p>\n\n\n\n

Multicolour Labelling of Mammalian Cells<\/h3>\n\n\n\n
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An image of the HeLa cell labelled with MBL fluorescent proteins targeting different organelles. mKO1 is localised on the plasma membrane (red), mAG1 in the endoplasmic reticulum (green) and mKeima-Red in the mitochondria (blue).<\/p>\n\n\n\n

The image and information are provided by Dr. Miyawaki Atsushi, Laboratory for Cell Function and Dynamics, BSI, RIKEN.<\/p>\n\n\n\n

Simultaneous four-color imaging of subcellular structures in a Vero cell using a single laser line (458 nm)<\/h2>\n\n\n\n
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Normalised excitation and emission spectra of SECFP, mMiCy, Venus and mKeima (a). An image of the Vero cell with SECFP localised on the plasma membrane (cyan), mMiCy in the endoplasmic reticulum (green), YFP along the microtubules (red) and mKeima in the mitochondria (purple) (b). The image was created by merging the following images, which were obtained using spectral imaging: SECFP-pm (c), mMiCy-er (d), Venus-mt (e) and mKeima-mit (f).<\/p>\n\n\n\n

Spectra imaging with a single laser line at 458 nm (Ar ion laser) was performed using the 32 channels of the LSM 510 META system (Carl Zeiss).<\/p>\n\n\n\n

Images were kindly provided by Takako Kogure, Atsushi Miyawaki (Laboratory for Cell Function and Dynamics, BSI, RIKEN).<\/p>\n\n\n\n

Mitophagy Detection<\/h2>\n\n\n\n

Keima-Red for mitophagy detection in living cells<\/h3>\n\n\n\n