{"id":1086,"date":"2025-05-29T17:02:00","date_gmt":"2025-05-29T17:02:00","guid":{"rendered":"http:\/\/www.caltagmedsystems.co.uk\/information\/?p=1086"},"modified":"2025-07-25T16:09:54","modified_gmt":"2025-07-25T16:09:54","slug":"dont-rip-your-hair-out-use-our-rip-certified-antibodies","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/dont-rip-your-hair-out-use-our-rip-certified-antibodies\/","title":{"rendered":"Don\u2019t rip your hair out- use our RIP certified antibodies!"},"content":{"rendered":"\n

What are RBPs (RNA binding proteins)? Very briefly, they are proteins that bind to RNA. (You probably figured that out already.) More importantly, they have a role in regulating RNA as part of the ribonucleoprotein complex (RNP). They are involved in interactions that regulate posttranscriptional gene regulation1<\/sup>. Not only are RBPs important because of their interactions, but also because it was found that mutations in RBPs can lead to disease formation 2, 3, 4<\/sup>. In order to investigate which RBPs bind with RNAs, a technique called RNP immunoprecipitation (RIP) can be used5<\/sup>. And in order to have an amazing RIP experiment, one needs an equally amazing antibody.<\/p>\n\n\n\n

The ENCODE Project- MBL Antibodies<\/h3>\n\n\n\n

Often times, a researcher will go on a hunt for an antibody. Typically, they will feel like they struck gold if they find a publication that used an antibody with their same cell type and somewhat similar functions. However, this is not always feasible. The company that manufactured the antibody may have tested a few different cell lines, but of course, may not have tested the one the researcher is using. Epigenesists now have a tool that can help researchers find that elusive, super awesome antibody. A collaborative team has put a lot of hard work into testing 700 commercially available antibodies from various vendors, included antibodies from MBL. This is known as the ENCODE Project. The team developed a database, where up-to-date results can be found here<\/a>.<\/p>\n\n\n\n

As of this blog publication, this group found 44 RBP antibodies supplied by MBL to work brilliantly! The top 5 best-selling RBP antibodies that are part of the validated antibodies are:<\/p>\n\n\n\n

Target<\/strong><\/td>Product Code<\/strong><\/td><\/tr>
EIF4G1<\/td>RN002P<\/a><\/td><\/tr>
IGF2BP1<\/td>RN007P<\/a><\/td><\/tr>
IGF2BP2<\/td>RN008P<\/a><\/td><\/tr>
IGF2BP3<\/td>RN009P<\/a><\/td><\/tr>
HNRNPK<\/td>RN019P<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

MBL\u2019s RIP certified antibodies are optimised to be used with RIP-Assay kit<\/a>. Using the\u00a0RIP certified antibodies and\/or MBL’s optimised kits, you\u2019ll be sure to find your favorite RBPs interacting with your favorite RNA.<\/p>\n\n\n\n

Questions? Contact Us!<\/a><\/p>\n\n\n\n

References<\/h4>\n\n\n\n

1. Castello, Alfredo, Rastislav Horos, Claudia Strein, Bernd Fischer, Katrin Eichelbaum, Lars M. Steinmetz, Jeroen Krijgsveld, and Matthias W. Hentze. “Comprehensive Identification of RNA-Binding Proteins by RNA Interactome Capture.” Methods in Molecular Biology Post-Transcriptional Gene Regulation (2016): 131-39.<\/p>\n\n\n\n

2. Yano, Masato, Yoshika Hayakawa-Yano, and Hideyuki Okano. “RNA Regulation Went Wrong in Neurodevelopmental Disorders: The Example of Msi\/Elavl RNA Binding Proteins.” International Journal of Developmental Neuroscience (2016):<\/p>\n\n\n\n

3. Goodwin, Marianne, and Maurice S. Swanson. “RNA-Binding Protein Misregulation in Microsatellite Expansion Disorders.” Systems Biology of RNA Binding Proteins Advances in Experimental Medicine and Biology (2014): 353-88.<\/p>\n\n\n\n

4. Castello, Alfredo, Bernd Fischer, Matthias W. Hentze, and Thomas Preiss. “RNA-binding Proteins in Mendelian Disease.” Trends in Genetics 29.5 (2013): 318-27.<\/p>\n\n\n\n

5. Keene, Jack D., Jordan M. Komisarow, and Matthew B. Friedersdorf. “RIP-Chip: The Isolation and Identification of MRNAs, MicroRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts.” Nat Protoc Nature Protocols 1.1 (2006): 302-07.<\/p>\n\n\n\n

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Caltag Medsystems <\/span><\/a>is the distributor of MBL’s<\/span> <\/a>products in the UK and Ireland. If you have any questions about these products, please contact<\/span><\/a> us<\/span><\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"

What are RBPs (RNA binding proteins)? Very briefly, they are proteins that bind to RNA. (You probably figured that out already.) More importantly, they have a role in regulating RNA as part of the ribonucleoprotein complex (RNP). They are involved<\/p>\n","protected":false},"author":13,"featured_media":12368,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[93],"tags":[98,272,96,97,95],"class_list":["post-1086","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-rna","tag-encode-project","tag-mbl","tag-rip-assay-kit","tag-rna-binding-proteins","tag-rna-immunoprecipitation"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/1086","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=1086"}],"version-history":[{"count":5,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/1086\/revisions"}],"predecessor-version":[{"id":12298,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/1086\/revisions\/12298"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media\/12368"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=1086"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=1086"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=1086"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}