{"id":1088,"date":"2016-05-03T13:24:16","date_gmt":"2016-05-03T13:24:16","guid":{"rendered":"http:\/\/www.caltagmedsystems.co.uk\/information\/?p=1088"},"modified":"2019-07-24T13:09:10","modified_gmt":"2019-07-24T13:09:10","slug":"improve-your-rna-research-using-our-anti-brdu-antibody","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/improve-your-rna-research-using-our-anti-brdu-antibody\/","title":{"rendered":"Improve your RNA research using our anti-BrdU antibody!"},"content":{"rendered":"

How do you quantify newly synthesised RNA? There are several ways to detect synthesised RNA, but these methods have their complications. The\u00a05-Bromouridine immunoprecipitation chase (BRIC)\u00a0Kit<\/a>\u00a0offers the\u00a0the most suitable method for RNA decay analysis.<\/em><\/p>\n

Regulation of gene expression by RNA degradation is a critical step for the coordination of many physiological processes. Decay pathways also play important roles in mRNA surveillance systems. Traditionally, transcriptional inhibitors such as Actinomycin D have been widely used to analyse RNA degradation. However, these methods have been shown to influence cellular physiology, including splicing, polyadenylation and other post transcriptional events.<\/p>\n

Recently, uridine analogues have been used to for genome-wide RNA decay analysis in mammalian cells, without the need for transcriptional\u00a0inhibitors. By incorporating uridine analogues into RNA such as BrU (5-Bromouridine), EU (5-Ethynyl Uridine) and 4sU (4-Thiouridine), labelled RNA can be isolated by immunoprecipitation to study the\u00a0stability and half-life of different RNAs.<\/p>\n

EU (5-Ethynyl Uridine) labelling methods require biotinylation of the labelled RNA to specifically collect the biotin-labelled RNA by streptavidin beads. This biotinylation step can be a trigger for RNA degradation, skewing the apparent stability of RNA.\u00a04sU (4-Thiouridine) permits base pairing with guanine instead of adenine, which causes base changes in the RNA sequence.<\/p>\n

BrU (5-Bromouridine) is less toxic\u00a0to cells than other uridine modifications and can be easily immunoprecipitated by the antibromodeoxyuridine (BruD) antibody<\/a> through its cross reactivity. Unlike EU and 4sU based analysis, the 5-Bromouridine immunoprecipitation chase (BRIC)<\/a> method avoids the mentioned\u00a0undesirable effects for downstream applications such as RT-qPCR, deep sequencing, or microarray, making it the most suitable method for RNA decay analysis.<\/p>\n

\"MBL<\/a>
BRIC Kit<\/a> assay principle, available from MBLI<\/figcaption><\/figure>\n

How can MBLI help? Anti-BrdU antibody and BRIC Kit<\/a> available<\/h4>\n

MBL International offers an anti-BrdU antibody<\/a> that has cross reactivity with BrU. Using this antibody, they have developed a comprehensive 5-bromouridine immunoprecipitation chase (BRIC) kit<\/a><\/strong> to detect RNA synthesis.<\/p>\n