{"id":11290,"date":"2024-10-17T14:12:04","date_gmt":"2024-10-17T14:12:04","guid":{"rendered":"https:\/\/www.caltagmedsystems.co.uk\/information\/?p=11290"},"modified":"2025-07-25T16:13:58","modified_gmt":"2025-07-25T16:13:58","slug":"what-are-the-differences-between-organoids-and-spheroids","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/what-are-the-differences-between-organoids-and-spheroids\/","title":{"rendered":"What are the Differences Between Organoids and Spheroids?"},"content":{"rendered":"\n

In general, there are two major differences:<\/p>\n\n\n\n

    \n
  1. Nature of the driving force for development:<\/strong> Whereas internal developmental processes drive organoid formation, spheroids develop primarily via cell-to-cell adhesion.<\/li>\n\n\n\n
  2. Length of time 3D cultures can be maintained:<\/strong> Long-term, in vitro<\/em> expansion of cells in culture needs an immature stem cell population to replenish dying cells. Organoids are derived from, and maintain, a population of stem cells during in vitro<\/em> culture, guaranteeing their long-term viability. This is achieved by optimising culture growth conditions, such as providing a basement membrane matrix (i.e. Matrigel\u00ae<\/sup>) and adding a selection of agonists (e.g. Wnt and tyrosine kinase receptor) and inhibitors (e.g. bone morphogenetic protein\/transforming growth factor-\u03b2).<\/li>\n<\/ol>\n\n\n\n

    Importantly, when organoids are passaged they retain the genetic features of the original organ over several generations. In contrast, the long-term culture of tissue-derived spheroids is challenging, possibly due to inherent technical difficulties in extracting and maintaining viable cells.<\/p>\n\n\n\n

    Organoids and spheroids can be generated from a variety of healthy as well as diseased cell types and tissues, such as patient tumours. Tumour-derived organoids and spheroids have been generated and extensively investigated for their use in drug discovery. However, there are some key differences in establishing the two from patient-derived tumours.<\/p>\n\n\n\n

    MBL International supports advances in cell culture by offering Afamin\/Wnt3a Condition Medium, recombinant Afamin\/Wnt3a and FGF-Max. Find out more:<\/strong><\/p>\n\n\n\n

    \n
    Afamin\/Wnt3a<\/strong><\/a><\/div>\n\n\n\n
    FGF-Max<\/strong><\/a><\/div>\n<\/div>\n\n\n\n
    <\/div>\n\n\n\n
    \n\n\n\n

    Information provided by MBL.<\/p>\n\n\n\n

    Caltag Medsystems <\/a>is the distributor of MBL <\/a>products in the UK and Ireland. If you have any questions about these products, please contact us<\/a>.<\/a><\/p>\n\n\n\n

    <\/p>\n","protected":false},"excerpt":{"rendered":"

    In general, there are two major differences: nature of the driving force for development and length of time 3D cultures can be maintained.<\/p>\n","protected":false},"author":13,"featured_media":12368,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18],"tags":[81,67,814,815],"class_list":["post-11290","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-cell-culture","tag-cell-culture","tag-mbl-international","tag-organoids","tag-spheroids"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/11290","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=11290"}],"version-history":[{"count":6,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/11290\/revisions"}],"predecessor-version":[{"id":12370,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/11290\/revisions\/12370"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media\/12368"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=11290"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=11290"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=11290"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}