{"id":11293,"date":"2024-10-17T15:30:08","date_gmt":"2024-10-17T15:30:08","guid":{"rendered":"https:\/\/www.caltagmedsystems.co.uk\/information\/?p=11293"},"modified":"2025-07-25T16:13:02","modified_gmt":"2025-07-25T16:13:02","slug":"fgf-max","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/fgf-max\/","title":{"rendered":"FGF-Max: Quality Assay Results"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">Quantitative luciferase reporter assay<\/h2>\n\n\n\n<p>A luciferase reporter assay (SRE Reporter, Luciferase, HEK293 Cell Line: BPS Bioscience) was performed to assess the activity and stability of FGF-Max.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">1. Comparing with FGF-2<\/h3>\n\n\n\n<div class=\"wp-block-group is-layout-grid wp-container-core-group-is-layout-b757edec wp-block-group-is-layout-grid\">\n<figure class=\"wp-block-image is-resized wp-container-content-5e2576f9\"><img decoding=\"async\" src=\"https:\/\/resources.mblintl.com\/hs-fs\/hubfs\/FGF_3-768x659.png?width=400&amp;name=FGF_3-768x659.png\" alt=\"\" style=\"width:201px;height:auto\"\/><\/figure>\n\n\n\n<p>FGF-Max and FGF-2 were added to the cells at different concentrations and cultured for 6 hours. The results show that FGF-Max has the same level of bio-activity as FGF-2.<\/p>\n<\/div>\n\n\n\n<h3 class=\"wp-block-heading clear\">2. Thermal stability evaluation<\/h3>\n\n\n\n<p>After pre-incubation of FGF-Max and FGF-2 at 37\u00b0C for 24 hours, 48 hours, and 72 hours, they were added to the cells for bio-activity assessment. The results suggested that FGF-Max retained higher bio-activity after pre-incubation compared to FGF-2, which also proves that FGF-Max has higher thermostability than FGF-2.<\/p>\n\n\n\n<figure class=\"wp-block-image is-resized\"><img decoding=\"async\" src=\"https:\/\/resources.mblintl.com\/hubfs\/FGF_4-1.png\" alt=\"\" style=\"width:707px;height:auto\"\/><\/figure>\n\n\n\n<h3 class=\"wp-block-heading clear\">3. Human small intestinal organoid cultures<\/h3>\n\n\n\n<p>Human small intestinal organoids were cultured in the presence of FGF-Max and FGF-2. The proliferation of organoids was higher in the presence of FGF-Max than in FGF-2.<\/p>\n\n\n\n<figure class=\"wp-block-image is-resized\"><img decoding=\"async\" src=\"https:\/\/resources.mblintl.com\/hubfs\/FGF-organoids.png\" alt=\"\" style=\"width:690px;height:auto\"\/><\/figure>\n\n\n\n<h3 class=\"wp-block-heading clear\">4. Differentiation of human small intestinal organoids<\/h3>\n\n\n\n<p>Paneth, endocrine and goblet cell markers of human small intestinal organoids cultured in the presence of FGF-Max (4 ng\/mL) were stained and the presence of these cells in organoids was confirmed.<\/p>\n\n\n\n<figure class=\"wp-block-image is-resized\"><img decoding=\"async\" src=\"https:\/\/resources.mblintl.com\/hubfs\/FGF-Paneth-cells-1.png\" alt=\"\" style=\"width:643px;height:auto\"\/><\/figure>\n\n\n\n<h2 class=\"wp-block-heading clear\">Establishment of mouse gastric organoids<\/h2>\n\n\n\n<p>Murine gastric organoids were cultured in the presence of FGF-Max and FGF-10. Similar to human small intestinal organoids, the proliferation of organoids was shown to be higher in the presence of FGF-Max.<\/p>\n\n\n\n<figure class=\"wp-block-image is-resized\"><img decoding=\"async\" src=\"https:\/\/resources.mblintl.com\/hubfs\/FGF-mouse-organoid.png\" alt=\"\" style=\"width:524px;height:auto\"\/><\/figure>\n\n\n\n<h3 class=\"wp-block-heading clear\">1. ATP assay<\/h3>\n\n\n\n<div class=\"wp-block-group is-layout-grid wp-container-core-group-is-layout-b757edec wp-block-group-is-layout-grid\">\n<figure class=\"wp-block-image is-resized\"><img decoding=\"async\" src=\"https:\/\/resources.mblintl.com\/hs-fs\/hubfs\/FGF_ATP-Assay-2.png?width=300&amp;name=FGF_ATP-Assay-2.png\" alt=\"\" style=\"width:170px;height:auto\"\/><\/figure>\n\n\n\n<p>ATP-assay results suggested that the cell viability in organoids is higher with the presence of FGF-Max.<\/p>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading clear\">Establishment of mouse pancreatic organoids<\/h2>\n\n\n\n<p>Murine pancreatic organoids were cultured in the presence of FGF-Max and FGF-10. Similar to the above results, it was suggested that the proliferation of organoids was higher in the presence of FGF-Max.<\/p>\n\n\n\n<figure class=\"wp-block-image is-resized\"><img decoding=\"async\" src=\"https:\/\/resources.mblintl.com\/hubfs\/FGF-mouse-pancreatic-organoids.png\" alt=\"\" style=\"width:498px;height:auto\"\/><\/figure>\n\n\n\n<p class=\"clear\">1. ATP assay<\/p>\n\n\n\n<div class=\"wp-block-group is-layout-grid wp-container-core-group-is-layout-b757edec wp-block-group-is-layout-grid\">\n<figure class=\"wp-block-image size-full is-resized\"><a href=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/image-477.png\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"372\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/image-477.png\" alt=\"\" class=\"wp-image-12339\" style=\"width:181px;height:auto\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/image-477.png 300w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/image-477-242x300.png 242w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/figure>\n\n\n\n<p>ATP-assay results suggested that the cell viability in organoids is higher with the presence of FGF-Max.<\/p>\n\n\n\n<p class=\"clear\"><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-buttons clear is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-a89b3969 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link has-text-color has-background wp-element-button\" href=\"https:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/index.php?group_1=All&amp;group_2=All&amp;supplier=10&amp;clonality=All&amp;host=All&amp;species=All&amp;applications=All&amp;regulatory_status=All&amp;searchwords=FGF-Max\" style=\"color:#fbc100;background-color:#21318f\"><strong>See all FGF-Max Products<\/strong><\/a><\/div>\n<\/div>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-alpha-channel-opacity has-background\" style=\"background-color:#21318f;color:#21318f\"\/>\n\n\n\n<p>Information originally posted on: <a href=\"https:\/\/www.mblbio.com\/bio\/g\/product\/cell-culture\/fgf-max.html\">https:\/\/www.mblbio.com\/bio\/g\/product\/cell-culture\/fgf-max.html<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.caltagmedsystems.co.uk\/\">Caltag Medsystems <\/a>is the distributor of <a href=\"https:\/\/www.caltagmedsystems.co.uk\/mbl\/\">MBL <\/a>products in the UK and Ireland. If you have any questions about these products, please <a href=\"https:\/\/www.caltagmedsystems.co.uk\/contact.php\">contact us<\/a>.<a href=\"\"><\/a><\/p>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>FGF-Max &#8211; a recombinant chimeric protein with features of both FGF-1 and FGF-2<\/p>\n","protected":false},"author":13,"featured_media":12368,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18,1],"tags":[81,759,67],"class_list":["post-11293","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-cell-culture","category-general-information","tag-cell-culture","tag-fgf-max","tag-mbl-international"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/11293","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=11293"}],"version-history":[{"count":13,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/11293\/revisions"}],"predecessor-version":[{"id":12345,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/11293\/revisions\/12345"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media\/12368"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=11293"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=11293"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=11293"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}