{"id":11452,"date":"2025-03-14T14:00:02","date_gmt":"2025-03-14T14:00:02","guid":{"rendered":"https:\/\/www.caltagmedsystems.co.uk\/information\/?p=11452"},"modified":"2025-03-18T15:54:35","modified_gmt":"2025-03-18T15:54:35","slug":"mhc-monomer-tetramer-product-validation-data","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/mhc-monomer-tetramer-product-validation-data\/","title":{"rendered":"KACTUS: MHC Monomer & Tetramer Product Validation Data"},"content":{"rendered":"\n

KACTUS has demonstrated, via ELISA and SPR binding assays, that their MHC-peptide complexes and loaded peptide-ready MHCs have good binding affinity to TCRs. Their MHC products are well-suited for applications such as antibody binding, affinity research, TCR screening, and blocking assays.<\/p>\n\n\n\n

\n
\"MHC
HLA-A*02:01 Tetramer & FMNKIYEI<\/strong>

Figure 1. Immobilized HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR at 2\u00b5g\/ml (100ul\/well) on the plate can bind\u00a0HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer<\/a>, His Tag\u00a0with an EC50 of 26.6ng\/ml determined by ELISA.<\/figcaption><\/figure>\n\n\n\n
\"MHC
Biotinylated HLA-A*02:01 Monomer & SLLMWITQV<\/strong>

Figure 2. Immobilized Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Antibody, hFc Tag at 1ug\/ml (100ul\/well) on the plate. Dose response curve for\u00a0Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Monomer<\/a>, His Tag with the EC50 of 31.3ng\/ml determined by ELISA.<\/figcaption><\/figure>\n<\/div>\n\n\n\n
\n
\"\"
HLA-G Tetramer & RIIPRHLQL<\/strong>
Figure 3. Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind\u00a0Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer<\/a>\u00a0with an affinity constant of 4.62 nM as determined in SPR assay (Biacore T200).<\/figcaption><\/figure>\n\n\n\n
\"MHC
HLA-A*02:01 Tetramer & YLEPGPVTA<\/strong>
Figure 4. In SPR analysis, the GP100 TCR & Anti-CD3 Bispecific Fusion can bind to the HLA-A*02:01&B2M&GP100 (YLEPGPVTA) complex tetramer, with an affinity constant of 0.196 nM.<\/figcaption><\/figure>\n<\/div>\n\n\n\n
\n
\"MHC
HLA-A*02:01 Monomer & FMNKFIYEI<\/strong>
Figure 5.\u00a0Human HLA-A*02:01&B2M&AFP (FMNKFIEl) Monomer<\/a>, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR with an affinity constant of 0.3991 \u00b5M as determined in SPR assay (Biacore T200).<\/figcaption><\/figure>\n\n\n\n
\"MHC
HLA-A*02:01 Tetramer & FMNKFIYEI<\/strong>
Figure 6.\u00a0Human HLA-A*02:01&B2M&AFP (FMNKFIEl) Tetramer<\/a>, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR with an affinity constant of 0.111 \u00b5M as determined in SPR assay (Biacore T200).<\/figcaption><\/figure>\n<\/div>\n\n\n\n
\n
\"MHC
PE-SA Assembled Tetramer with SIINFEKL, OT1 Mouse Whole Blood<\/strong>
Figure 7. Flow Cytometry Analysis of OT1 Mouse Whole Blood Cells and T Cells Using PE-SA Assembled Tetramers. The right panel displays the binding of OT1 mouse T cells to the Mouse H-2K(b) & B2M & OVA (SIINFEKL) PE-SA assembled tetramer, with a significant shift in fluorescence and higher percentage of MHC PE-H positive cells (39.60% in Q5-4), compared to the left control, demonstrating effective binding and tetramer assembly.<\/figcaption><\/figure>\n\n\n\n
\"MHC
PE-SA Assembled Tetramer with SIINFEKL, Peptide-Pulsed OT1 Mouse Spleen Blood<\/strong>
Figure 8. Flow Cytometry Analysis of SIINFEKL Peptide-Pulsed OT1 Mouse Spleen Cells Using PE-SA Assembled Tetramers. The right panel displays the binding of OT1 mouse spleen cells to the custom-assembled tetramer (Mouse H-2K(b) & B2M & OVA (SIINFEKL) PE-SA), with a significant shift in fluorescence and higher percentage of MHC PE-H positive cells (97.41% in Q5-4), demonstrating effective binding and tetramer assembly.<\/figcaption><\/figure>\n<\/div>\n\n\n\n
\n\n\n\n

Caltag Medsystems\u00a0<\/a>is the distributor of\u00a0KACTUS products in the UK and Ireland. If you have any questions about these products, please\u00a0contact us<\/a>.<\/p>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

KACTUS has demonstrated via ELISA & SPR binding assays that their MHC-peptide complexes & loaded peptide-ready MHCs have good binding affinity to TCRs.<\/p>\n","protected":false},"author":13,"featured_media":11448,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1,15],"tags":[976,900,978,224],"class_list":["post-11452","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-general-information","category-tetramer","tag-kactus","tag-monomer","tag-product-validation","tag-tetramer"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/11452","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=11452"}],"version-history":[{"count":7,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/11452\/revisions"}],"predecessor-version":[{"id":11696,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/11452\/revisions\/11696"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media\/11448"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=11452"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=11452"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=11452"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}