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Enzymes for every step of RNA synthesis<\/h2>\n\n\n\nRestriction Endonucleases for Plasmid Linearisation<\/h3>\n\n\n\n
The template for mRNA in vitro transcription is typically plasmid DNA, and needs to be linearised before transcription. Linearisation ensures the acquisition of mRNA transcripts of definite length and sequence. KACTUS provides\u00a0restriction endonucleases\u00a0such as\u00a0BsaI<\/a>,\u00a0BspQI<\/a>,\u00a0SapI<\/a>,\u00a0XbaI<\/a>, etc., for the preparation of linearised templates.<\/p>\n\n\n\n <\/a><\/p>\n\n\n\n In Vitro Transcription (IVT) is a technique that generates mRNA by mimicking the internal transcription process enzymatically, using linearised plasmid DNA as a template.<\/p>\n\n\n\n <\/a>Note: MaxPure\u2122 T7 RNA Polymerase is the commercial name for Premium T7 RNA Polymerase (RUO\u00a0or\u00a0GMP)<\/p>\n\n\n\n The mRNA produced by IVT has a triphosphate group at the 5\u2019 end, which has strong immunogenicity, necessitating capping and tailing modifications in industrial production. KACTUS offers\u00a0Vaccinia Capping Enzyme\u00a0and\u00a0mRNA Cap 2\u00b4-O-Methyltransferase\u00a0needed for capping modifications, as well as\u00a0E.coli Poly(A) Polymerase\u00a0required for tailing modifications. To precisely quantify important indicators such as “capping rate” and “Poly(A) tail product length,” they have carefully established an LC-MS platform and developed mature mass spectrometry detection methods to control the release of enzyme products by batch. They can also provide capping rate and tailing determination services according to your needs.<\/p>\n\n\n\n Linear mRNA obtained from IVT can be further circularised to obtain circRNA. The circularisation methods include Type I intron self-splicing, Type II intron self-splicing, T4 RNA Ligase, etc. KACTUS provides\u00a0T4 RNA Ligase I,\u00a0T4 RNA Ligase II, and\u00a0RNase R\u00a0for in vitro RNA circularisation and purification processing of circRNA.<\/p>\n\n\n\n Figure 5. RNase R digests linear RNA (left) without affect circRNA (right).<\/p>\n\n\n\n Caltag Medsystems\u00a0<\/a>is the distributor of\u00a0KACTUS products in the UK and Ireland. If you have any questions about these products, please\u00a0contact us<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":" KACTUS have used their SAMS\u2122 protein production platform to develop high-quality enzymes for in vitro synthesis of mRNA (GMP-Grade & GMP-Ready).<\/p>\n","protected":false},"author":13,"featured_media":11448,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[987,1,93],"tags":[923,976,994],"class_list":["post-11572","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-enzymes","category-general-information","category-rna","tag-enzymes","tag-kactus","tag-rna-production"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/11572","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=11572"}],"version-history":[{"count":7,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/11572\/revisions"}],"predecessor-version":[{"id":11685,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/11572\/revisions\/11685"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media\/11448"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=11572"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=11572"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=11572"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}![\"\"\/](\"https:\/\/kactusbio.com\/cdn\/shop\/files\/BsaI_Combined.png?v=1696285084&width=1000\")
In Vitro Transcription (IVT) Reagents<\/h3>\n\n\n\n
Available Products:<\/h4>\n\n\n\n
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![\"\"](\"https:\/\/kactusbio.com\/cdn\/shop\/files\/MaxPure_T7_dsRNA_withLegend.png?v=1721066336&width=1000\")
Enzymes for Capping & Tailing<\/h3>\n\n\n\n
Available Products:<\/h4>\n\n\n\n
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![\"\"](\"https:\/\/kactusbio.com\/cdn\/shop\/files\/Capping_Combined.png?v=1696286049&width=1000\")
<\/a>Enzymes for circRNA In Vitro Synthesis<\/h3>\n\n\n\n
Available Products:<\/h4>\n\n\n\n
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![\"\"](\"https:\/\/kactusbio.com\/cdn\/shop\/files\/RNaseR1.png?v=1677592580&width=1604\")
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