{"id":1164,"date":"2016-06-02T14:56:41","date_gmt":"2016-06-02T14:56:41","guid":{"rendered":"http:\/\/www.caltagmedsystems.co.uk\/information\/?p=1164"},"modified":"2016-06-02T15:12:05","modified_gmt":"2016-06-02T15:12:05","slug":"transficient-and-sirfficient-superior-transfection-activity-against-other-commercial-reagents-in-numerous-adherent-cell-types","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/transficient-and-sirfficient-superior-transfection-activity-against-other-commercial-reagents-in-numerous-adherent-cell-types\/","title":{"rendered":"Transficient\u2122 and siRfficient\u2122: Superior Transfection Activity against other Commercial Reagents in Numerous Adherent Cell Types"},"content":{"rendered":"<p>Transficient\u2122 and siRfficient\u2122 from <a href=\"http:\/\/www.caltagmedsystems.co.uk\/mbl\/\" target=\"_blank\">MBL International<\/a> are transfection reagents that provide superior transfection efficiency for primary cells and a wide variety of cell lines, including those \u201chard-to-transfect\u201d cell lines. They are a cost effective and highly efficient tool for your downstream applications using a simple transfection protocol.<\/p>\n<h5>Transficient\u2122 : DNA Transfection Reagent<\/h5>\n<p>This product has been developed and optimised for delivery of nucleic acids, such as plasmid DNA and mRNA into a wide variety of cell types by creating highly stable nanoparticles that facilitates cellular uptake, endosomal escape and localization to the nucleus.<\/p>\n<p><strong>Key benefits:<\/strong><\/p>\n<ul>\n<li>High transfection efficiency into primary cells and a wide variety of cell lines<\/li>\n<li>Works with or without serum<\/li>\n<li>Easy to use<\/li>\n<li>Robust<\/li>\n<li>Very low toxicity<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/TransFicient.png\" rel=\"attachment wp-att-1159\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1159\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/TransFicient.png\" alt=\"TransFicient\" width=\"940\" height=\"172\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/TransFicient.png 940w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/TransFicient-300x55.png 300w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/TransFicient-768x141.png 768w\" sizes=\"auto, (max-width: 940px) 100vw, 940px\" \/><\/a><\/p>\n<p><em>Five different cell lines were transfected using the Luciferase gene and the Transficient\u2122\u00a0reagent. Expression levels were compared to competitor transfection reagents.<\/em><\/p>\n<p><strong>Products:<\/strong><\/p>\n\n<table id=\"tablepress-17\" class=\"tablepress tablepress-id-17\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\">Product Code<\/th><th class=\"column-2\">Product description<\/th><th class=\"column-3\">Pack Size<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=690082\" target=\"_blank\">WU1001<\/a><\/td><td class=\"column-2\">Transficient\u2122 DNA Transfection Reagent SAMPLE<\/td><td class=\"column-3\">1ml<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=690083\" target=\"_blank\">WU1002<\/a><\/td><td class=\"column-2\">Transficient\u2122 Transfection Reagent<\/td><td class=\"column-3\">3ml <\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=690084\" target=\"_blank\">WU1003<\/a><\/td><td class=\"column-2\">Transficient\u2122 Transfection Reagent<\/td><td class=\"column-3\">10ml<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=690085\" target=\"_blank\">WU1004<\/a><\/td><td class=\"column-2\">Transficient\u2122 Transfection Reagent<\/td><td class=\"column-3\">6 x 10ml<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=690086\" target=\"_blank\">WU1005<\/a><\/td><td class=\"column-2\">Transficient\u2122 Transfection Reagent<\/td><td class=\"column-3\">12 x 10ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-17 from cache -->\n<p>Transficient\u2122 DNA Transfection Reagent samples available,\u00a0please email <a href=\"mailto:office@caltagmedsystems.co.uk\">office@caltagmedsystems.co.uk<\/a> to request.<\/p>\n<h5>siRfficient\u2122: siRNA Transfection Reagent<\/h5>\n<p>This product has been developed and optimised for gene silencing experiments. It provides the highest efficiencies for the delivery of siRNA, microRNA or antisense RNA into the cytoplasm of a wide variety of cell types, including Neuro 2a, Jurkat, K562 and LLC-1cells.<\/p>\n<p><strong>Key benefits:<\/strong><\/p>\n<ul>\n<li>High transfection efficiency into primary cells and a wide variety of cell lines<\/li>\n<li>Works with or without serum<\/li>\n<li>Easy to use<\/li>\n<li>Robust<\/li>\n<li>Very low toxicity<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/siRfficient.png\" rel=\"attachment wp-att-1160\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1160\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/siRfficient.png\" alt=\"siRfficient\" width=\"940\" height=\"172\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/siRfficient.png 940w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/siRfficient-300x55.png 300w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/siRfficient-768x141.png 768w\" sizes=\"auto, (max-width: 940px) 100vw, 940px\" \/><\/a><\/p>\n<p><em>Left:\u00a0\u03b2-actin levels were knocked down in Jurkat cells using the siRfficient\u2122 reagent. Compared to Company A and Company B, siRfficient\u2122 showed a 90% knockdown level. Right:\u00a0VEGF protein levels were silenced using the siRfficient\u2122 reagent, with and without serum in a Neuro 2a cell line. The results were compared Competitor silencing reagents.<\/em><\/p>\n<p><strong>Products:<\/strong><\/p>\n\n<table id=\"tablepress-18\" class=\"tablepress tablepress-id-18\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\">Product Code<\/th><th class=\"column-2\">Product description<\/th><th class=\"column-3\">Pack Size<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=690087\" target=\"_blank\">WU2001<\/a><\/td><td class=\"column-2\">siRfficient\u2122 siRNA Transfection Reagent SAMPLE<\/td><td class=\"column-3\">1ml<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=690088\" target=\"_blank\">WU2002<\/a><\/td><td class=\"column-2\">siRfficient\u2122 siRNA Transfection Reagent<\/td><td class=\"column-3\">3ml <\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=690089\" target=\"_blank\">WU2003<\/a><\/td><td class=\"column-2\">siRfficient\u2122 siRNA Transfection Reagent<\/td><td class=\"column-3\">10ml<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=690090\" target=\"_blank\">WU2004<\/a><\/td><td class=\"column-2\">siRfficient\u2122 siRNA Transfection Reagent<\/td><td class=\"column-3\">6 x 10ml<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=690091\" target=\"_blank\">WU2005<\/a><\/td><td class=\"column-2\">siRfficient\u2122 siRNA Transfection Reagent<\/td><td class=\"column-3\">12 x 10ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-18 from cache -->\n<p>siRNA Transfection Reagent samples available, please email <a href=\"mailto:office@caltagmedsystems.co.uk\">office@caltagmedsystems.co.uk<\/a> to request.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Transficient\u2122 and siRfficient\u2122 from MBL International are transfection reagents that provide superior transfection efficiency for primary cells and a wide variety of cell lines, including those \u201chard-to-transfect\u201d cell lines. They are a cost effective and highly efficient tool for your<\/p>\n","protected":false},"author":13,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18,1],"tags":[81,67,80,79],"class_list":["post-1164","post","type-post","status-publish","format-standard","hentry","category-cell-culture","category-general-information","tag-cell-culture","tag-mbl-international","tag-sirifficient","tag-transficient"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/1164","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=1164"}],"version-history":[{"count":7,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/1164\/revisions"}],"predecessor-version":[{"id":1177,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/1164\/revisions\/1177"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=1164"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=1164"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=1164"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}