{"id":11750,"date":"2025-03-25T11:52:11","date_gmt":"2025-03-25T11:52:11","guid":{"rendered":"https:\/\/www.caltagmedsystems.co.uk\/information\/?p=11750"},"modified":"2025-03-25T11:52:12","modified_gmt":"2025-03-25T11:52:12","slug":"flow-cytometry-antibodies-comparison","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/flow-cytometry-antibodies-comparison\/","title":{"rendered":"Flow Cytometry Antibodies Comparison"},"content":{"rendered":"\n

Elabscience conducted comparison studies between their Flow Cytometry antibodies and antibodies from BioLegend and BD Biosciences. The below data shows comparable quality between each manufacturer.<\/p>\n\n\n\n

Elabscience vs BioLegend<\/h2>\n\n\n\n
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Fig.1. C57BL\/6 murine splenocytes were stained with APC-conjugated anti-mouse CD4 antibodies<\/strong> [GK1.5]<\/strong> from Elabscience (E-AB-F1097E<\/strong><\/a>) and BioLegend, using both 0.25T and 1T for each brand.<\/figcaption><\/figure>\n\n\n\n
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Fig.2. C57BL\/6 murine splenocytes were stained with PerCP-conjugated anti-mouse CD4 antibodies [GK1.5]<\/strong> from Elabscience (E-AB-F1097F<\/strong><\/a>) and BioLegend, using both 0.25T and 1T for each brand.<\/figcaption><\/figure>\n\n\n\n
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Fig.3. C57BL\/6 murine splenocytes were stained with PE-conjugated anti-mouse CD4 antibodies [GK1.5]<\/strong> from Elabscience (E-AB-F1097D<\/strong><\/a>) and BioLegend, using both 0.25T and 1T for each brand.<\/figcaption><\/figure>\n\n\n\n
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Fig.4. C57BL\/6 murine splenocytes were stained with PE\/Cyanine7-conjugated anti-mouse CD4 antibodies [GK1.5]<\/strong> from Elabscience (E-AB-F1097H<\/strong><\/a>) and BioLegend, using both 0.25T and 1T for each brand.<\/figcaption><\/figure>\n\n\n\n
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Fig.5. C57BL\/6 murine splenocytes were stained with PerCP\/Cyanine5.5-conjugated anti-mouse CD4 antibodies [GK1.5]<\/strong> from Elabscience (E-AB-F1097J<\/strong><\/a>) and BioLegend, using both 0.25T and 1T concentrations for each brand.<\/figcaption><\/figure>\n\n\n\n
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Fig.6. C57BL\/6 murine splenocytes were stained with APC-conjugated anti-mouse CD90.2\/Thy1.2 antibodies [30H12]<\/strong> from Elabscience (E-AB-F1094E<\/strong><\/a>) and BioLegend, using 1T concentrations for each brand.<\/figcaption><\/figure>\n\n\n\n
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Fig.7. C57BL\/6 murine splenocytes were stained with FITC-conjugated anti-mouse CD4 antibodies [GK1.5]<\/strong> from Elabscience (E-AB-F1097C<\/strong><\/a>) and BioLegend, using 1T concentrations for each brand.<\/figcaption><\/figure>\n\n\n\n
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Fig.8. C57BL\/6 murine splenocytes were stained with Elab Fluor\u00ae 488-conjugated anti-mouse CD4 antibodies<\/strong> from Elabscience (E-AB-F1097L<\/strong><\/a>) and BioLegend, using 1T concentrations for each brand.<\/figcaption><\/figure>\n\n\n\n
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Fig.9. C57BL\/6 murine splenocytes were stained with APC-conjugated anti-mouse CD62L antibodies<\/strong> from Elabscience (E-AB-F1011E<\/strong><\/a>) and BioLegend, using 1T concentrations for each brand.<\/figcaption><\/figure>\n\n\n\n
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Fig.10. C57BL\/6 murine platelets were stained with APC-conjugated anti-mouse CD41 antibodies<\/strong> from Elabscience (E-AB-F1183E<\/strong><\/a>) and BioLegend, using 1T concentrations for each brand.<\/figcaption><\/figure>\n\n\n\n
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Fig.11. Rat splenocytes were stained with PE-conjugated anti-rat CD8a antibodies<\/strong> from Elabscience (E-AB-F1098D<\/strong><\/a>) and BioLegend, using 1T concentrations for each brand.<\/figcaption><\/figure>\n<\/div>\n\n\n\n

Elabscience vs BD Biosciences<\/h2>\n\n\n\n
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Fig.12. Normal human peripheral blood cells were stained with PE-conjugated anti-human CD4 antibodies [RPA-T4]<\/strong> from Elabscience (E-AB-F1109D<\/strong><\/a>) and BD, using 1T concentrations for each brand. Cells in the lymphocytes gate were used for analysis.<\/figcaption><\/figure>\n\n\n\n
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Fig.13. Normal human peripheral blood cells were stained with PE-conjugated anti-human CD8a antibodies<\/strong> from Elabscience (E-AB-F1110D<\/strong><\/a>) and BD, using 1T concentrations for each brand. Cells in the lymphocytes gate were used for analysis.<\/figcaption><\/figure>\n\n\n\n
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Fig.14. Normal human peripheral blood cells were stained with PE-conjugated anti-human CD56\/NCAM antibodies<\/strong> from Elabscience (E-AB-F1305D<\/strong><\/a>) and BD, using 1T concentrations for each brand. Cells in the lymphocytes gate were used for analysis.<\/figcaption><\/figure>\n\n\n\n
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Fig.15. Normal human peripheral blood cells were stained with PE-conjugated anti-human CD4 antibodies<\/strong> from Elabscience (E-AB-F1352D<\/strong><\/a>) and BD, using 1T concentrations for each brand. Cells in the lymphocytes gate were used for analysis. Comparison of PE Anti Human CD4 Antibody and BD products from different batches.<\/figcaption><\/figure>\n<\/div>\n\n\n\n
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Information provided by Elabscience<\/a>.<\/p>\n\n\n\n

Caltag Medsystems <\/a>is the distributor of Elabscience <\/a>products in the UK and Ireland.<\/p>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

Comparison study data between Flow Cytometry antibodies from Elabscience, BioLegend and BD Biosciences.<\/p>\n","protected":false},"author":13,"featured_media":7262,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[132,17,1],"tags":[477,46,913],"class_list":["post-11750","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-antibodies","category-flow-cytometry","category-general-information","tag-elabscience","tag-flow-cytometry","tag-flow-cytometry-antibodies"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/11750","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=11750"}],"version-history":[{"count":30,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/11750\/revisions"}],"predecessor-version":[{"id":11797,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/11750\/revisions\/11797"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media\/7262"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=11750"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=11750"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=11750"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}