{"id":11875,"date":"2025-04-07T20:45:04","date_gmt":"2025-04-07T20:45:04","guid":{"rendered":"https:\/\/www.caltagmedsystems.co.uk\/information\/?p=11875"},"modified":"2025-04-07T20:56:15","modified_gmt":"2025-04-07T20:56:15","slug":"pcr-polymerases","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/pcr-polymerases\/","title":{"rendered":"PCR Polymerases"},"content":{"rendered":"\n

abm’s pursuit of next generation PCR enzymes has led to intensive R&D and significant upgrades to their PCR products. The result – a new collection of world leading PCR enzymes and formulations with performance far beyond the current leading brands.<\/p>\n\n\n\n

Products Available:<\/h3>\n\n\n\n
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PCR Sure\u2122 Kit<\/a><\/h4>\n\n\n\n

MegaFi\u2122 Pro Fidelity DNA Polymerase<\/a><\/h4>\n\n\n\n

Kodaq 2X PCR MasterMix<\/a><\/h4>\n\n\n\n
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BlasTaq\u2122 DNA Polymerase<\/a><\/h4>\n\n\n\n

BlasTaq\u2122 HotStart DNA Polymerase<\/a><\/h4>\n\n\n\n

Ultra-Pure BlasTaq\u2122 2X PCR MasterMix<\/a><\/h4>\n<\/div>\n\n\n\n
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PCR Sure\u2122 Kit<\/h2>\n\n\n\n
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Simplify the PCR optimisation process with our PCR Sure\u2122 Kit. With a system consisting of multiple thermostable DNA polymerases pre-mixed with optimised buffers, your PCR is guaranteed to work!<\/p>\n\n\n\n

PRODUCT<\/th>CAT. NO.<\/th><\/tr><\/thead>
PCR Sure\u2122 Kit<\/td>ABM-G065<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Amplifies Difficult Template<\/p>\n\n\n\n

Saves PCR Optimisation Time<\/p>\n\n\n\n

Guaranteed to Work<\/p>\n<\/div>\n\n\n\n

Super Versatile<\/h3>\n\n\n\n

Amplify your difficult templates with abm\u2019s PCR Sure\u2122 Kit. From crude templates to purified plasmids, High GC to Low GC, human, mouse, rat or plant DNA, one of their highly optimised mastermixes is sure to work!<\/p>\n\n\n\n

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Extremely Easy to Use<\/h3>\n\n\n\n

With 8 optimised 2X MasterMixes, simply add your primers and template and run on one single program!<\/p>\n\n\n\n

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MegaFi\u2122 Pro Fidelity DNA Polymerase<\/h2>\n\n\n\n
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MegaFi\u2122 Pro Fidelity DNA Polymerase is the best high fidelity DNA Polymerase on the market. With 2000X better fidelity than regular Taq, this ultrasensitive polymerase amplifies even the most difficult templates.<\/p>\n\n\n\n

PRODUCT<\/th>CAT. NO.<\/th><\/tr><\/thead>
MegaFi\u2122 Pro Fidelity DNA Polymerase<\/td>ABM-G886<\/a><\/td><\/tr>
MegaFi\u2122 Fidelity 2X PCR MasterMix<\/td>ABM-G887<\/a><\/td><\/tr>
MegaFi\u2122 Pro One-Step RT-PCR *<\/td>ABM-G597<\/a><\/td><\/tr>
*Contains OneScript\u2122 Hot Reverse Transcriptase (Cat. No. ABM-G593<\/a>)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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MegaFi\u2122 Pro Fidelity<\/p>\n\n\n\n

Amplifies difficult templates<\/p>\n\n\n\n

Effective in long-range PCR<\/p>\n<\/div>\n\n\n\n

Ultra-Low Error Rates<\/h3>\n\n\n\n
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MegaFi\u2122 Pro DNA Polymerase is THE BEST high fidelity DNA polymerase<\/strong> A lacZa-containing sequence was amplified using various DNA polymerases, subcloned, and plated on X-gal-containing media. Colonies without PCR amplified lacZa mutations appear dark blue, while those that have PCR introduced frameshift mutations appear white.<\/p>\n\n\n\n

Superior Fidelity<\/h3>\n\n\n\n
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MegaFi\u2122 Pro Fidelity DNA Polymerase has ~2000X higher fidelity than other enzymes on the market. <\/strong>abm\u2019s MegaFi\u2122 Pro Fidelity DNA Polymerase (Cat. No. ABM-G886<\/a>) has the highest accuracy rate compared to other DNA polymerases as tested using blue\/white colony screening. Shown as relative fidelity compared to Taq DNA Polymerase (Taq = 1X). PCR cycle number and mutations resulting in non-phenotypic changes are accounted for.<\/p>\n\n\n\n

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Kodaq 2X PCR MasterMix<\/h2>\n\n\n\n
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Kodaq 2X PCR MasterMix has a ready-to-use formula which includes a uniquely-formulated buffer with gel loading dye and strategically engineered mutations for robust, high-fidelity performance. The buffer accommodates high AT and GC content and common PCR inhibitors found in DNA samples.<\/p>\n\n\n\n

PRODUCT<\/th>CAT. NO.<\/th><\/tr><\/thead>
Kodaq 2X PCR MasterMix<\/td>ABM-G883<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Long Range PCR<\/p>\n\n\n\n

Highly Robust<\/p>\n\n\n\n

Superior sensitivity<\/p>\n<\/div>\n\n\n\n

Highly Robust<\/h3>\n\n\n\n
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PCR amplification of a GC-Rich 1.4 kbp plasmid template using abm\u2019s Kodaq 2X PCR MasterMix (Cat. No. ABM-G883<\/a>) (column 1) vs. competitor products (column 2 to 5).<\/p>\n\n\n\n

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PCR amplification of difficult templates (including plant gDNA) using abm\u2019s Kodaq 2X PCR MasterMix (Cat. No. ABM-G883<\/a>) (column 1) vs. competitor products (column 2 and 3). The red arrows indicate the expected product sizes.<\/p>\n\n\n\n

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BlasTaq\u2122 DNA Polymerase<\/h2>\n\n\n\n
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Blast through a routine 2 hour PCR run in only 20 minutes with abm’s BlasTaq\u2122 DNA Polymerase. Reach extension speeds as fast as 6 kb\/min and streamline your PCR!<\/p>\n\n\n\n

PRODUCT<\/th>CAT. NO.<\/th><\/tr><\/thead>
BlasTaq\u2122 DNA Polymerase<\/td>ABM-G894<\/a><\/td><\/tr>
BlasTaq\u2122 2X PCR MasterMix<\/td>ABM-G895<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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6 kb\/min extension speed<\/p>\n\n\n\n

40-70% less time than Taq<\/p>\n\n\n\n

Superior sensitivity<\/p>\n<\/div>\n\n\n\n

Super Fast Extension Speeds<\/h3>\n\n\n\n
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BlasTaq\u2122 DNA Polymerase enables extension speeds as fast as 6 kb\/min.<\/strong> Total reaction times for abm<\/strong>\u2019s BlasTaq\u2122 DNA Polymerase (Cat. No. ABM-G894<\/a>) and Taq were determined for the amplification of different sized amplicons: 500 bp, 1 kb, 2 kb, and 5 kb. Reaction times are based on a 30-cycle program using the recommended reaction protocol for each enzyme.<\/p>\n\n\n\n

Highly Robust<\/h3>\n\n\n\n
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PCR amplification using abm<\/strong>\u2019s BlasTaq\u2122 DNA Polymerase (Cat. No. ABM-G894<\/a>) (column 1) of various targets vs. competitor polymerases (column 2 and 3) followed by electrophoresis on a 1% agarose gel. A) 1 kb target from Human cDNA. B) 1.3 kb target from plasmid DNA. C) 7.6 kb target from plasmid DNA.<\/p>\n\n\n\n

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BlasTaq\u2122 HotStart DNA Polymerase<\/h2>\n\n\n\n
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Eliminate non-specific amplification and primer-dimer formation with abm’s next generation BlasTaq\u2122 HotStart DNA Polymerase. This formulation includes a proprietary antibody that blocks polymerase activity at low temperatures and dissociates at the denaturation step to restore enzyme activity.<\/p>\n\n\n\n

PRODUCT<\/th>CAT. NO.<\/th><\/tr><\/thead>
BlasTaq\u2122 HotStart DNA Polymerase<\/td>ABM-G595<\/a><\/td><\/tr>
BlasTaq\u2122 HotStart 2X PCR MasterMix<\/td>ABM-G598<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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No non-specific amplification or primer dimers<\/p>\n\n\n\n

Improves yield of desired product<\/p>\n\n\n\n

Simplifies reaction setup<\/p>\n<\/div>\n\n\n\n

Excellent for suspension cells<\/h3>\n\n\n\n
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BlasTaq\u2122 HotStart DNA Polymerase eliminates non-specific amplification. <\/strong>abm\u2019s BlasTaq\u2122 HotStart DNA Polymerase (Cat. No. ABM-G595<\/a>) was used to amplify a 7 kb target. The antibody concentration was decreased incrementally from lanes 1-5. Lane 1 is their BlasTaq\u2122 HotStart formulation and lane 5 contains no antibody.<\/p>\n\n\n\n

Super Fast Extension Speeds<\/h3>\n\n\n\n
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BlasTaq\u2122 HotStart DNA Polymerase has extension speeds of 6 kb\/min. <\/strong>abm\u2019s BlasTaq\u2122 HotStart DNA Polymerase (Cat. No. ABM-G595<\/a>) was used to amplify different sized amplicons (500 bp, 1 kb, 2 kb, and 5 kb). Total reaction times are listed based on a 30-cycle program using the recommended reaction protocol for each enzyme.<\/p>\n\n\n\n

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Ultra-Pure BlasTaq\u2122 2X PCR MasterMix<\/h2>\n\n\n\n
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Take your PCR to the next level with Ultra-Pure BlasTaq\u2122 2X PCR MasterMix<\/strong>. This formulation includes the same strategically-engineered, next generation Taq Polymerase which was subject to rigorous multi-step purification protocol using physical, chemical, and enzymatic methods for maximum removal of contaminating genomic DNA.<\/p>\n\n\n\n

PRODUCT<\/th>CAT. NO.<\/th><\/tr><\/thead>
Ultra-Pure BlasTaq\u2122 2X PCR MasterMix<\/td>ABM-G885<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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High purity<\/p>\n\n\n\n

6 kb\/min extension speed<\/p>\n\n\n\n

40-70% less time than Taq<\/p>\n<\/div>\n\n\n\n

High Purity<\/h3>\n\n\n\n
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Ultra-Pure BlasTaq\u2122 PCR MasterMix ensures efficient amplification while maintaining low-DNA contamination levels when compared to leading competitors.<\/strong> Two different primer sets (blue and orange) against host DNA were tested with two competitor products (1 and 2) and abm\u2019s Ultra-Pure BlasTaq\u2122 enzyme (3). There is no amplification seen in the no-template controls (NTC) from Ultra-Pure BlasTaq\u2122 PCR MasterMix indicating exceptional purity of the product. Activity Control Primer Set demonstrated similar performance for all three tested products.<\/p>\n\n\n\n

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Unsure which polymerase is right for you?<\/h2>\n\n\n\n
CHARACTERISTIC<\/th>MegaFi\u2122 Pro Fidelity<\/th>BlasTaq\u2122<\/th>BlasTaq\u2122 HotStart<\/th>Ultra-Pure BlasTaq\u2122<\/th>Taq<\/th>Kodaq<\/th><\/tr><\/thead>
Proofreading<\/td>Yes<\/td>No<\/td>No<\/td>No<\/td>No<\/td>Yes<\/td><\/tr>
Fidelity (vs. Native Taq)<\/td>1300X<\/td>1X<\/td>1X<\/td>1X<\/td>1X<\/td>50X<\/td><\/tr>
Specificity<\/td>\u2022\u2022\u2022\u2022<\/td>\u2022\u2022\u2022<\/td>\u2022\u2022\u2022\u2022<\/td>\u2022\u2022\u2022<\/td>\u2022\u2022\u2022<\/td>\u2022\u2022\u2022\u2022<\/td><\/tr>
Extension Speed<\/td>20-30 sec\/kb<\/td>15 sec\/kb<\/td>15 sec\/kb<\/td>15 sec\/kb<\/td>60 sec\/kb<\/td>60 sec\/kb<\/td><\/tr>
Max Target Length<\/td>15-20 kb<\/td>10-15 kb<\/td>10-15 kb<\/td>10-15 kb<\/td>6.5 kb<\/td>15-20 kb<\/td><\/tr>
Suitable for TA cloning<\/td>No<\/td>Yes<\/td>Yes<\/td>Yes<\/td>Yes<\/td>No<\/td><\/tr>
Special Feature<\/td>Fidelity and Sensitivity<\/td>Fast and Robust<\/td>High Specificity<\/td>High Purity<\/td>For routine PCR<\/td>Fidelity and Highly Robust<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Information originally posted on: https:\/\/www.abmgood.com\/Taq-Polymerase.html<\/a><\/p>\n\n\n\n

Caltag Medsystems <\/a>is the distributor of abm products in the UK and Ireland. If you have any questions about these products, please contact us<\/a>.<\/p>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

abm\u2019s pursuit of next-generation PCR enzymes has led to intensive R&D and significant upgrades to their PCR products.<\/p>\n","protected":false},"author":13,"featured_media":12052,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[677,1,755],"tags":[1008,1007,1015,1012,292,1013,1014],"class_list":["post-11875","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-dna","category-general-information","category-pcr-kits","tag-abm","tag-applied-biological-materials","tag-dna-polymerase","tag-mastermix","tag-pcr","tag-pcr-polymerase","tag-polymerase"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/11875","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=11875"}],"version-history":[{"count":52,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/11875\/revisions"}],"predecessor-version":[{"id":12072,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/11875\/revisions\/12072"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media\/12052"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=11875"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=11875"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=11875"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}