{"id":11992,"date":"2025-04-07T20:54:03","date_gmt":"2025-04-07T20:54:03","guid":{"rendered":"https:\/\/www.caltagmedsystems.co.uk\/information\/?p=11992"},"modified":"2025-04-07T20:54:04","modified_gmt":"2025-04-07T20:54:04","slug":"transfection-reagents","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/transfection-reagents\/","title":{"rendered":"Transfection Reagents"},"content":{"rendered":"\n

abm\u2019s transfection reagents are unique formulations designed to aid the delivery of nucleic acids into a variety of cell lines with high transfection efficiency and low cell toxicity.<\/p>\n\n\n\n

Their collection includes DNAfectin\u2122 Plus for transection into adherent cells, and Susfectin\u2122 for transfection of suspension 293 and CHO cells in the production of recombinant proteins, antibodies, and recombinant viruses.<\/p>\n\n\n\n

Products Available:<\/h3>\n\n\n\n
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DNAfectinTM<\/sup> Plus<\/a><\/h4>\n\n\n\n

SusfectinTM<\/sup><\/a><\/h4>\n<\/div>\n\n\n\n
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DNAFectinTM<\/sup> Plus DNA Transfection Reagents<\/h2>\n\n\n\n
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abm\u2019s DNAfectinTM<\/sup> Plus out-performs competitors in transfection efficiency for a variety of popular cell lines. In addition, it has added benefit of performing transfection without the need to remove serum\/culture medium.<\/p>\n\n\n\n

PRODUCT<\/th>CAT. NO.<\/th><\/tr><\/thead>
DNAfectinTM<\/sup> Plus Transfection Reagent*<\/td>ABM-G2500<\/a><\/td><\/tr>
*Serum-compatible<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Minimal toxicity<\/p>\n\n\n\n

Out-performs in a range of cells<\/p>\n\n\n\n

Serum compatible<\/p>\n<\/div>\n\n\n\n

Out-performs competitors<\/h3>\n\n\n\n
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DNAfectinTM<\/sup>\u00a0Plus out-performs competitors in transfection efficiency for a variety of popular cell lines.<\/strong>\u00a0DNAfectinTM<\/sup>\u00a0Plus Transfection Reagent (Cat. No. ABM-G2500<\/a>) or a competitor\u2019s transfection reagent were used to deliver a GFP construct into CHO-K1, HeLa, HEK-293T or Calu-6 cells. Images were taken 24 hours post-transfection.<\/p>\n\n\n\n

No medium changes needed<\/h2>\n\n\n\n
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DNAfectinTM<\/sup>\u00a0Plus enables transfection in the presence of serum.<\/strong>\u00a0HEK-293T Cells were transfected with a GFP construct using DNAfectin\u2122 Plus (Cat. No. ABM-G2500<\/a>) or a competitor’s transfection reagent. DNAfectin\u2122 Plus does not require removal of serum\/culture medium during the transfection, as the transfection mixture can be added directly to complete medium.<\/p>\n\n\n\n

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To Top<\/strong><\/a><\/div>\n<\/div>\n\n\n\n
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SusfectinTM<\/sup> Suspension Cell Transfection Reagent<\/h2>\n\n\n\n
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abm’s SusfectinTM<\/sup> Transfection Reagent is uniquely formulated for transfection of DNA into eukaryotic suspension cells without the need to remove serum\/culture medium. This simplified workflow makes SusfectinTM<\/sup> the best choice for transfection of CHO and 293 cells.<\/p>\n\n\n\n

PRODUCT<\/th>CAT. NO.<\/th><\/tr><\/thead>
Susfectin\u2122 Transfection Reagent*<\/sup><\/td>ABM-G4000<\/a><\/td><\/tr>
*Serum-compatible<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Best choice for suspension cells (CHO, 293)<\/p>\n\n\n\n

Minimal toxicity<\/p>\n\n\n\n

Serum compatible<\/p>\n<\/div>\n\n\n\n

Excellent for suspension cells<\/h3>\n\n\n\n
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abm’s Susfectin\u2122 Transfection Reagent enables high efficiency transfection of suspension cells.<\/strong>\u00a0HEK293 suspension cells was transfected with a Scrambled siRNA GFP Lentivector (Cat. No. ABM-LV015-G<\/a>) using Susfectin\u2122 Transfection Reagent (Cat. No. ABM-G4000<\/a>).<\/p>\n\n\n\n

2X higher expression<\/h3>\n\n\n\n
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Susfectin\u2122 results in 2X higher expression compared to PEI.<\/strong>\u00a0CHO cells were transfected using Susfectin\u2122 (Cat. No. ABM-G4000<\/a>), a competing transfection reagent, or linear PEI. Recombinant antibody levels in the supernatant were quantified using ELISA.<\/p>\n\n\n\n

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Information originally posted on: https:\/\/www.abmgood.com\/Transfection-Reagent-Protocol-Efficiency.html<\/a><\/p>\n\n\n\n

Caltag Medsystems <\/a>is the distributor of abm products in the UK and Ireland. If you have any questions about these products, please contact us<\/a>.<\/p>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

abm\u2019s unique transfection reagents are designed to aid the delivery of nucleic acids into a variety of cell lines with high transfection efficiency and low cell toxicity.<\/p>\n","protected":false},"author":13,"featured_media":12052,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1,438],"tags":[1008,1007,1019,1020,864],"class_list":["post-11992","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-general-information","category-transfection","tag-abm","tag-applied-biological-materials","tag-dnafectin","tag-susfectin","tag-transfection-reagents"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/11992","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=11992"}],"version-history":[{"count":8,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/11992\/revisions"}],"predecessor-version":[{"id":12070,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/11992\/revisions\/12070"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media\/12052"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=11992"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=11992"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=11992"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}