{"id":1208,"date":"2016-06-06T14:03:01","date_gmt":"2016-06-06T14:03:01","guid":{"rendered":"http:\/\/www.caltagmedsystems.co.uk\/information\/?p=1208"},"modified":"2024-04-17T12:10:47","modified_gmt":"2024-04-17T12:10:47","slug":"high-quality-green-fluorescent-protein-gfp-antibodies","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/high-quality-green-fluorescent-protein-gfp-antibodies\/","title":{"rendered":"High Quality Green Fluorescent Protein (GFP) Antibodies"},"content":{"rendered":"

Nordic-MUbio<\/a><\/span> have recently released a new range of products directed against the\u00a0native form of GFP. Nordic’s\u00a0anti-GFP antibodies bring a number of advantages\u00a0over antibodies produced against recombinant, or variant forms of\u00a0GFP.<\/p>\n

This includes the ability to detect GFP produced from a wide range\u00a0of different sources, including native, recombinant, and a number of\u00a0different mutants, without suffering from high levels of non-specific\u00a0binding\/background.<\/p>\n

Part of the reason many anti-recombinant GFP antibodies suffer from\u00a0high background issues is due to contamination of an innoculum\u00a0with other E.coli components, leading to increased non-specific\u00a0binding.<\/p>\n

Nordic’s products were tested for cross-reactivity to a wide range of\u00a0antigens, including many derived from E.coli, and demonstrated no\u00a0cross-reactivity.<\/p>\n

Available Products:<\/strong><\/p>\n

Rabbit anti-GFP\u00a0(native) –\u00a0Ammonium Sulfate\u00a0fraction (0.1 mg)<\/a><\/span><\/p>\n

Rabbit anti-GFP\u00a0(native) – Protein A Purified (0.1 mg)<\/a><\/span><\/p>\n

Rabbit anti-GFP\u00a0(native) – Affinity Purified (0.1 mg)<\/a><\/span><\/p>\n

Rabbit anti-GFP\u00a0(native):PO –\u00a0Soyabean Peroxidase\u00a0Conjugate (0.1 ml)<\/a><\/span><\/p>\n

Chicken anti-GFP\u00a0(native) –\u00a0Ammonium Sulfate\u00a0fraction (0.1 mg)<\/a><\/span><\/p>\n

Recombinant\u00a0Green Fluorescent\u00a0Protein (wild type) –\u00a0Recombinant Protein (0.1 mg)\u00a0<\/a><\/span><\/p>\n

To ensure Nordic-MUbio\u2019s new GFP antibody range is of the highest\u00a0quality, a number of comparison experiments were run with the bestselling\u00a0antibodies from other suppliers against recombinant GFP,
\nassessing purity, specificity, and titre of the antibodies:<\/p>\n

\"nordic_GFP\"<\/a><\/p>\n

Example Size Exclusion\u00a0Chromatography (HPLC) Results:\"GFP_Nordic\"<\/a><\/p>\n

The HPLC analyses of the Nordic-MUbio affinity purified antibodies\u00a0show just a single peak, illustrating\u00a0their purity. The competitor\u00a0antibodies with multiple peaks\u00a0have contaminants, such as serum\u00a0albumin, which can affect the results\u00a0of e.g. Western Blots or ELISAs.<\/p>\n

\"Nordic-GFP\"<\/a><\/p>\n

Background Information<\/strong><\/p>\n

Green Fluorescent Protein\u00a0(GFP) is a 27kD protein derived\u00a0from the Aequorea victoria (A.victoria) jellyfish. It is a naturally\u00a0fluorescent protein which has\u00a0revolutionised life science and\u00a0has been used to study proteins\u00a0in a wide range of applications.\u00a0GFP has been used extensively\u00a0to monitor gene expression; to\u00a0localise proteins at the cellular\u00a0and sub-cellular level; to track\u00a0metastatic cells and as a tag to\u00a0identify proteins in vitro<\/em>.<\/p>\n

 <\/p>\n

A. victoria<\/em> is a bioluminescent jellyfish, or hydromedusa, found off the west coast of North America.<\/p>\n

The enormous interest in GFP\u00a0as a protein marker has given\u00a0rise to the development of polyclonal and monoclonal\u00a0antibodies against GFP, used\u00a0for the immunolocalisation\u00a0and immunoblotting of GFPfused\u00a0proteins in a variety of\u00a0applications.<\/p>\n

Since its discovery, many variants\u00a0of GFP have been engineered\u00a0making antibodies recognising a\u00a0wide range of these variants an\u00a0important and useful scientific\u00a0tool for use alongside GFP-fused\u00a0proteins.<\/p>\n

Nordic-MUbio’s anti-GFP antibodies are distributed in the UK & ROI by Caltag Medsystems. For more information about this range, or any other products available from Nordic-NUbio, please contact us<\/a><\/span> and we will be happy to help.<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Nordic-MUbio have recently released a new range of products directed against the\u00a0native form of GFP. Nordic’s\u00a0anti-GFP antibodies bring a number of advantages\u00a0over antibodies produced against recombinant, or variant forms of\u00a0GFP. This includes the ability to detect GFP produced from a<\/p>\n","protected":false},"author":13,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[29],"tags":[281],"class_list":["post-1208","post","type-post","status-publish","format-standard","hentry","category-immunology","tag-nordic-mubio"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/1208","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=1208"}],"version-history":[{"count":10,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/1208\/revisions"}],"predecessor-version":[{"id":4994,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/1208\/revisions\/4994"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=1208"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=1208"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=1208"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}