{"id":12200,"date":"2025-05-14T12:23:20","date_gmt":"2025-05-14T12:23:20","guid":{"rendered":"https:\/\/www.caltagmedsystems.co.uk\/information\/?p=12200"},"modified":"2025-05-14T12:25:41","modified_gmt":"2025-05-14T12:25:41","slug":"easymers-peptide-loadable-mhc-class-i","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/easymers-peptide-loadable-mhc-class-i\/","title":{"rendered":"easYmers\u00ae peptide loadable MHC class I"},"content":{"rendered":"\n<h3 class=\"wp-block-heading\">With immunAware&#8217;s peptide receptive easYmers\u00ae, you can build a MHC class I repository relevant to your project and start producing pMHC complexes in your own lab. There are more than 50 HLA allotypes to choose from!<\/h3>\n\n\n\n<p>All MHC class I easYmer\u00ae molecules are biotinylated allowing you to make tetramers with your preferred labels. Stored frozen, MHC class I easYmers\u00ae are stable and peptide receptive for 24 months; once complexed with peptides, the resulting monomers are stable for years in the freezer.<\/p>\n\n\n\n<p>immunAware recommend that you use predictors (e.g.&nbsp;<a href=\"https:\/\/services.healthtech.dtu.dk\/services\/NetMHCcons-1.1\/\" target=\"_blank\" rel=\"noreferrer noopener\">NetMHC series<\/a>&nbsp;or&nbsp;<a href=\"https:\/\/services.healthtech.dtu.dk\/services\/NetMHCpan-4.1\/\" target=\"_blank\" rel=\"noreferrer noopener\">NetMHCpan<\/a>) as an aid to design your monomers. If the specific peptide-MHC class I combination of interest is not predicted to bind with a sufficiently low %Rank, there\u2019s a high risk that the peptide will not support the formation of stable complexes. Thus, to ensure proper peptide loading and optimal staining of your CD8+ T cells, they strongly recommend that you experimentally verify successful peptide-MHC (pMHC) complex formation. To this end, the easYmer kit includes a simple FACS based protocol allowing you to validate proper peptide loading (<a href=\"https:\/\/immunaware.com\/wp-content\/uploads\/2024\/08\/Assessment-of-HLA-complex-folding-FACS-assay.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">click here for protocol<\/a>).<\/p>\n\n\n\n<p>The peptide loaded MHC monomers can be long-term stored frozen for later tetramerisation. When needed, these monomers can easily be tetramerised with labelled streptavidin (e.g. fluorophore label for analysis in a classical flow cytometry assay or metal-labels for analysis by CyTOF). For optimal HLA tetramer staining of CD8+ T cells, please follow their <a href=\"https:\/\/immunaware.com\/wp-content\/uploads\/2024\/08\/Tetramer-staining-of-human-CD8-T-cells.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">MHC class I tetramer staining protocol<\/a>.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\"><strong>The easYmers\u00ae technology is highly flexibility and suitable for:<\/strong><\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Evaluation of peptide-MHC class I interactions.<\/li>\n\n\n\n<li>Epitope \/ Neo-epitope screening \u2013 small-scale tetramer production for screening of a large number of different epitopes in parallel.<\/li>\n\n\n\n<li>Donor screening \u2013 large-scale tetramer production for screening of a single epitope in a large number of (donor) samples.<\/li>\n\n\n\n<li>50+ HLA-A | HLA-B | HLA-C allotypes available<\/li>\n\n\n\n<li>Mouse H2-Db, H2-Kb available<\/li>\n<\/ul>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-a89b3969 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link has-text-color has-background has-link-color wp-element-button\" href=\"https:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/index.php?group_1=All&amp;group_2=All&amp;supplier=155&amp;clonality=All&amp;host=All&amp;species=All&amp;applications=All&amp;regulatory_status=All&amp;searchwords=easYmer\" style=\"color:#fbc100;background-color:#21318f\"><strong>See immunAware&#8217;s range of available easYmers\u00ae<\/strong><\/a><\/div>\n<\/div>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full clear\"><a href=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/easYmers-1000x575-downloaded-from-website.png\"><img loading=\"lazy\" decoding=\"async\" width=\"1000\" height=\"575\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/easYmers-1000x575-downloaded-from-website.png\" alt=\"\" class=\"wp-image-12202\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/easYmers-1000x575-downloaded-from-website.png 1000w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/easYmers-1000x575-downloaded-from-website-300x173.png 300w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/easYmers-1000x575-downloaded-from-website-768x442.png 768w\" sizes=\"auto, (max-width: 1000px) 100vw, 1000px\" \/><\/a><\/figure>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-alpha-channel-opacity has-background clear\" style=\"margin-top:var(--wp--preset--spacing--50);margin-bottom:var(--wp--preset--spacing--50);background-color:#21318f;color:#21318f\"\/>\n\n\n\n<p>Originally posted on: <a href=\"https:\/\/immunaware.com\/technology\/easymer\/\">https:\/\/immunaware.com\/technology\/easymer\/<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.caltagmedsystems.co.uk\/\">Caltag Medsystems\u00a0<\/a>is the distributor of\u00a0<a href=\"https:\/\/www.caltagmedsystems.co.uk\/immunaware\/\">immunAware\u00a0<\/a>products in the UK and Ireland. If you have any questions about these products, please\u00a0<a href=\"https:\/\/www.caltagmedsystems.co.uk\/contact.php\">contact<\/a>\u00a0<a href=\"https:\/\/www.caltagmedsystems.co.uk\/contact.php\">us<\/a>.<\/p>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>With immunAware&#8217;s peptide receptive easYmers\u00ae, you can produce pMHC complexes tailored for your project, in your own lab!<\/p>\n","protected":false},"author":13,"featured_media":12234,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[17,29,15],"tags":[1035,50,685,224],"class_list":["post-12200","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-flow-cytometry","category-immunology","category-tetramer","tag-immunaware","tag-immunology","tag-t-cells-2","tag-tetramer"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/12200","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=12200"}],"version-history":[{"count":6,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/12200\/revisions"}],"predecessor-version":[{"id":12256,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/12200\/revisions\/12256"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media\/12234"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=12200"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=12200"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=12200"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}