{"id":12600,"date":"2026-04-09T13:12:15","date_gmt":"2026-04-09T13:12:15","guid":{"rendered":"https:\/\/www.caltagmedsystems.co.uk\/information\/?p=12600"},"modified":"2026-04-09T13:12:16","modified_gmt":"2026-04-09T13:12:16","slug":"rt-pcr-rt-qpcr-mixes-data","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/rt-pcr-rt-qpcr-mixes-data\/","title":{"rendered":"RT-PCR & RT-qPCR Mixes Data"},"content":{"rendered":"\n

DB RT-PCR Mixes<\/h3>\n\n\n\n

In a customer study, the relative expression of two Arabidopsis thaliana genes was analysed under different light conditions. Quantitative RT-PCR was performed using DIANA’s DB RT-PCR SYBR Mix and a leading competitor mix. Melting curve analysis revealed multiple non-specific products for each target with the competitor mix, sometimes completely failing to amplify the specific product. In contrast, DB RT-PCR SYBR Mix produced a single, specific amplicon for each target. The high specificity of DIANA’s mixes simplifies primer design and ensures reliable quantification across experiments.<\/p>\n\n\n\n

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DBdirect™ RT-PCR Mixes<\/h3>\n\n\n\n

CCRF-CEM human cells were FACS-sorted directly into PCR plates containing 10 \u03bcL of RT-PCR mix and processed by one-step RT-qPCR.<\/p>\n\n\n\n

Two transcripts (GAPDH and TBP) were detected using either sequence-specific TaqMan probes or SYBR Green I dye.<\/p>\n\n\n\n

DBdirect RT-PCR mixes enabled precise mRNA quantification from single cells without RNA extraction, allowing rapid, cost-efficient RNA profiling at single-cell resolution.<\/p>\n\n\n\n

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Information provided by DIANA Biotechnologies.<\/p>\n\n\n\n

Caltag <\/a>is the distributor of DIANA Biotechnologies\u2019 products in the UK and Ireland. If you have any questions about these products, please contact<\/a> us<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"

DB RT-PCR Mixes and DBdirect™ RT-PCR Mixes are the ultimate solution for efficient and reproducible analysis.<\/p>\n","protected":false},"author":13,"featured_media":12599,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1058,995,755],"tags":[1060,1059,304,520],"class_list":["post-12600","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-master-mixes","category-molecular-biology","category-pcr-kits","tag-dbdirect","tag-diana-biotechnologies","tag-rt-pcr","tag-rt-qpcr"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/12600","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=12600"}],"version-history":[{"count":2,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/12600\/revisions"}],"predecessor-version":[{"id":12604,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/12600\/revisions\/12604"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media\/12599"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=12600"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=12600"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=12600"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}