{"id":12731,"date":"2026-06-08T15:31:09","date_gmt":"2026-06-08T15:31:09","guid":{"rendered":"https:\/\/www.caltagmedsystems.co.uk\/information\/?p=12731"},"modified":"2026-06-08T15:47:31","modified_gmt":"2026-06-08T15:47:31","slug":"find-the-right-crispr-vector-format","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/find-the-right-crispr-vector-format\/","title":{"rendered":"Find the right CRISPR vector format"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">Our supplier, <strong>abm<\/strong>, is proud to offer an expanded line of CRISPR-related products and services, including a genome-wide collection of CRISPR Cas9 sgRNA-expressing vectors and viruses for efficient gene knockout experiments. Each human, mouse, or rat gene is targeted with a set of three sgRNAs, available across Lentiviral, AAV, and non-viral formats.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Depending on your project requirements, the sgRNA may be delivered together with Cas9 in a single plasmid (All-in-One Vector) or separately in a two-plasmid (Dual Vector) format.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Read the guide below to help you choose the right format for your research!<\/p>\n\n\n\n<div class=\"wp-block-group is-layout-grid wp-container-core-group-is-layout-f4cb68d0 wp-block-group-is-layout-grid\" style=\"border-style:none;border-width:0px;border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-left-radius:0px;border-bottom-right-radius:0px;margin-top:0;margin-bottom:0;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0\">\n<h2 class=\"wp-block-heading has-text-align-left wp-container-content-b7d18107\"><br>Lentiviral<\/h2>\n\n\n\n<h2 class=\"wp-block-heading wp-container-content-b7d18107\"><br>AAV<\/h2>\n\n\n\n<h2 class=\"wp-block-heading\"><br>Non-viral<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Integrate into the genome, providing stable Cas9\/sgRNA expression.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Deliver episomal DNA, but have a limited packaging capacity (~4.7 kb). abm&#8217;s vectors use the smaller saCas9 variant to fit within this size limit.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Provide transient expression, reducing off-target effects. Non-viral methods include: Plasmid transfection (Cas9 + sgRNA), mRNA delivery (Cas9 mRNA + synthetic sgRNA), RNP complexes (Cas9 protein pre-complexed with sgRNA)<\/p>\n\n\n\n<h6 class=\"wp-block-heading\">Ideal for:<\/h6>\n\n\n\n<h6 class=\"wp-block-heading\">Ideal for:<\/h6>\n\n\n\n<h6 class=\"wp-block-heading\">Ideal for:<\/h6>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Stable, long-term knockout<\/li>\n\n\n\n<li>Working with hard-to-transfect (e.g., suspension cells, primary T cells), stem cells or dividing cell lines<\/li>\n\n\n\n<li>Generating pooled or arrayed CRISPR knockout libraries for functional genomics and drug screening<\/li>\n<\/ul>\n\n\n\n<ul class=\"wp-block-list\">\n<li>In vivo knockout studies due to their low immunogenicity and tissue-specific tropism<\/li>\n\n\n\n<li>Targeting post-mitotic cells such as neurons and muscle cells<\/li>\n<\/ul>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Short-term functional assays<\/li>\n\n\n\n<li>Rapid, transient knockouts with minimal genomic integration risk<\/li>\n<\/ul>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-3e41869c wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"https:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/?group_1=All&amp;group_2=All&amp;supplier=153&amp;clonality=All&amp;host=All&amp;species=All&amp;applications=All&amp;regulatory_status=All&amp;searchwords=abm-LG\"><strong>See Lentiviral KO Vectors<\/strong><\/a><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-3e41869c wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"https:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/?group_1=All&amp;group_2=All&amp;supplier=153&amp;clonality=All&amp;host=All&amp;species=All&amp;applications=All&amp;regulatory_status=All&amp;searchwords=abm-VG\"><strong>See AAV KO Vectors<\/strong><\/a><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-3e41869c wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"https:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/?group_1=All&amp;group_2=All&amp;supplier=153&amp;clonality=All&amp;host=All&amp;species=All&amp;applications=All&amp;regulatory_status=All&amp;searchwords=abm-PG\"><strong>See Non-Viral KO Vectors<\/strong><\/a><\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">All-in-One Lentiviral Vectors<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">An All-in-One vector system offers easy, one-step transfection with increased transfection efficiency and 1:1 sgRNA and Cas9 expression.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">abm\u2019s All-in-One Lentiviral Vector is a leading tool for CRISPR gene knockout, enabling sgRNA expression from the U6 promoter and expression of Cas9 and Puromycin (can be modified to other markers) selection from the SFFV promoter.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">This single-vector system streamlines experiments by leveraging lentiviral delivery across diverse cell types and requiring only one construct to achieve precise gene knockout.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">abm\u2019s All-in-One Lentiviral Vector Sets have been successfully utilised for gene knockout by researchers around the world and have been published in high-impact journals such as Nature Communications and Nature Microbiology. Together, these features make abm\u2019s CRISPR Cas9 tools a robust and versatile solution for streamlined, high-confidence gene knockout workflows.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Dual Vector System<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">A Dual Vector system is suitable for multiplex experiments, as a pool of several different sgRNA vectors can be transfected alongside Cas9-expressing constructs.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">abm\u2019s Dual Vector system comprises a Cas9-expressing construct and a separate construct carrying the sgRNA (driven by the U6 promoter) as well as the Neomycin resistance (controlled by a PGK promoter)<br>for downstream screening. <\/p>\n\n\n\n<p class=\"wp-block-paragraph\">A multiplex sgRNA CRISPR vector is also available, which allows for the expression of up to 4 sgRNAs from a single construct.<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\" style=\"margin-top:var(--wp--preset--spacing--50);margin-bottom:var(--wp--preset--spacing--50)\"\/>\n\n\n\n<p class=\"wp-block-paragraph\">Information provided by abm.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/www.caltagmedsystems.co.uk\/\">Caltag&nbsp;<\/a>is the distributor of&nbsp;<a href=\"https:\/\/www.caltagmedsystems.co.uk\/applied-biological-materials\/\">abm <\/a>products in the UK and Ireland. If you have any questions about these products, please&nbsp;<a href=\"https:\/\/www.caltagmedsystems.co.uk\/contact.php\">contact us<\/a>.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>abm is proud to offer a genome-wide collection of CRISPR Cas9 sgRNA-expressing vectors and viruses. Read the guide to help you choose the right format for your research!<\/p>\n","protected":false},"author":13,"featured_media":12758,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[997,1008,1007,199,1099,1100,1098],"class_list":["post-12731","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-general-information","tag-aav","tag-abm","tag-applied-biological-materials","tag-crispr","tag-lentiviral","tag-non-viral","tag-vectors"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/12731","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=12731"}],"version-history":[{"count":26,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/12731\/revisions"}],"predecessor-version":[{"id":12759,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/12731\/revisions\/12759"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media\/12758"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=12731"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=12731"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=12731"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}