{"id":12809,"date":"2026-07-17T15:12:29","date_gmt":"2026-07-17T15:12:29","guid":{"rendered":"https:\/\/www.caltagmedsystems.co.uk\/information\/?p=12809"},"modified":"2026-07-17T15:12:31","modified_gmt":"2026-07-17T15:12:31","slug":"organoid-culture-tools","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/organoid-culture-tools\/","title":{"rendered":"Organoid Culture Tools"},"content":{"rendered":"\n

Matrices, Scaffolds & Hydrogels<\/h2>\n\n\n\n

Increase the accuracy of\u00a0in vitro<\/em>\u00a0models with realistic culture microenvironments<\/h5>\n\n\n\n
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Metatissue<\/strong> <\/a>uses ethically sourced human proteins, from blood & placentas, to produce biomaterials (hydrogels, sponges & bioinks<\/a><\/strong>) with mechanically tunable properties.<\/p>\n\n\n\n

Use these biomaterials to create next-generation xeno-free biomimetic 3D cell culture platforms, personalised to experimental needs & without animal-derived supplements.<\/p>\n\n\n\n

✨ Batch-to-batch variability remains a major challenge for Matrigel, often affecting experimental consistency. A recent study demonstrates that hPLMA maintains reproducible cell performance across different batches, supporting sustained hASC viability, proliferation and metabolic activity while promoting a more homogeneous cell distribution.<\/p>\n\n\n\n

These findings highlight the potential of hPLMA as a reliable human-derived alternative for robust and reproducible 3D cell culture. Read the full paper.<\/a> ✨<\/p>\n\n\n\n

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Maintain the\u00a0in vivo<\/em>\u00a0morphology of your cells & improve research relevance<\/h5>\n\n\n\n
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Gelatex<\/strong> <\/a>manufactures Gelacell™<\/a><\/strong>, a non-woven, highly porous nanofiber-based scaffold for in vitro<\/em> 3D cell culture, tissue engineering & organoid scaffolds.<\/p>\n\n\n\n

Gelacell™ is produced using halospinning technology<\/strong> \u2013 the fastest and most cost-effective solution-spinning process \u2013 and offers exceptional biocompatibility and non-toxicity across a variety of cell types.<\/p>\n\n\n\n

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Simulates the microenvironment of human tissue using synthetic biological materials<\/h5>\n\n\n\n
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Leadgene\u00ae<\/strong><\/a> ScaffoldForm 3D Culturing Gel<\/a><\/strong> is a 3D cell culture hydrogel system that enhances cell growth and viability, providing you with more reliable and reproducible results.<\/p>\n\n\n\n

It is stable, adjustable, easy to operate, and has high biological compatibility, which can be applied in 3D cell & organoid culture, tumour formation, tumour angiogenesis and invasion studies.<\/p>\n\n\n\n

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Media (inc. specialised formulations)<\/h2>\n\n\n\n

Pioneering chemically-defined, animal-free tissue culture to empower stem cell research, regenerative medicine and advanced cell-based applications<\/h5>\n\n\n\n
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CELLnTEC<\/strong><\/a>‘s product highlights for organoid culture include:<\/p>\n\n\n\n

CnT-NX-E –<\/a><\/strong> The next-generation successor to CELLnTEC\u2019s market-leading precision media, CnT-07 and CnT-PR, designed for the isolation and expansion of epithelial cells from skin, Eye, gingiva, mammary, and bladder tissue. CnT-NX-E offers markedly improved expansion rates, robustness, and versatility across primary cells, iPSC-derived cells, and cell lines. For differentiation experiments, switching to CnT-PR-D or CnT-PR-3D differentiation media is recommended.<\/p>\n\n\n\n

CnT-PR-AD –<\/a><\/strong> A chemically defined formulated medium designed for the differentiation of large airway epithelial cells in 2D or 3D culture. CnT-PR-AD is completely free of animal- or human-derived components and does not contain phenol red or antibiotics\/antimycotics. It is used for differentiating airway epithelial cells that have been expanded in CnT-PR-A or CnT-17 media.<\/p>\n\n\n\n

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Enable long-term organoid culture<\/h5>\n\n\n\n
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Wnt3a is a common growth factor for organoid cultures, but this lipid-soluble protein tends to aggregate over time in serum-free media, leading to a loss of activity. Mihara et al. found that Afamin, a component of serum, forms a complex with Wnt3a to maintain Wnt3a’s high activity. The use of an Afamin-Wnt3a complex enables the long-term culture of organoids.<\/p>\n\n\n\n

Utilising this knowledge, MBL<\/a> <\/strong>has developed an Afamin\/Wnt3a Conditioned Media<\/a><\/strong> to be added to the culture media of intestinal organoids! Also available is a high-concentration<\/a><\/strong> version, for higher density, higher activity and reduced costs in organoid culture.<\/p>\n\n\n\n

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Media & supplements for the culture of Stem Cells<\/h5>\n\n\n\n
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Choosing the right products for your experiments is crucial to ensuring your cells grow well and maintain activity. With Caltag, gain access to high-quality culture media and supplements<\/a><\/strong> from a range of suppliers to ensure you can find the right formulation for your stem cells’ needs.<\/p>\n\n\n\n

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Biological Samples<\/h2>\n\n\n\n

The building blocks of organoids<\/h5>\n\n\n\n
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\"StemBioSys<\/a><\/figure>\n<\/div>\n\n\n\n

Stem Cells<\/strong><\/a> derived from the adipose, hepatic, nervous, oral, placental, pulmonary, skeletal, skeletal muscle and umbilical cell systems.<\/p>\n\n\n\n

Progenitor Cells<\/a><\/strong> derived from the dermal, hepatic, nervous, ocular, oral and urinary systems.<\/p>\n\n\n\n

Epithelial Cells<\/a><\/strong> derived from the auditory, dermal, endocrine, gastrointestinal, hepatic, lymphatic, mammary, nervous, ocular, oral, placental, pulmonary, reproductive and urinary cell systems.<\/p>\n\n\n\n

✨ Use our Primary Cell Finder Tool<\/a><\/strong> to browse all the cell types available! ✨<\/p>\n\n\n\n

iPSCs & hPSCs<\/strong><\/a> including astrocytes, neural stem cells, neurons, endothelial cells and more.<\/p>\n\n\n\n

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The one-stop hepatic in vitro<\/em> model<\/h5>\n\n\n\n
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HepaRG\u00ae<\/a><\/strong> from Biopredic International<\/a><\/strong> is a reliable, robust, and well-published human hepatic stem cell line with unique abilities to fully differentiate into mature and functional hepatocytes.<\/p>\n\n\n\n