{"id":1378,"date":"2016-07-14T13:45:27","date_gmt":"2016-07-14T13:45:27","guid":{"rendered":"http:\/\/www.caltagmedsystems.co.uk\/information\/?p=1378"},"modified":"2025-03-17T13:24:36","modified_gmt":"2025-03-17T13:24:36","slug":"fmh-quikquant-a-rapid-assay-for-fetomaternal-hemorrhage-quantification","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/fmh-quikquant-a-rapid-assay-for-fetomaternal-hemorrhage-quantification\/","title":{"rendered":"FMH QuikQuant &#8211; A Rapid assay for Fetomaternal Hemorrhage Quantification"},"content":{"rendered":"<p><a href=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/FMH_QuikQuant_box.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignright size-medium wp-image-3829\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/FMH_QuikQuant_box-300x200.jpg\" alt=\"IQ Products FMH QuikQuant FMH detection and quantification \" width=\"300\" height=\"200\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/FMH_QuikQuant_box-300x200.jpg 300w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/FMH_QuikQuant_box-768x512.jpg 768w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/FMH_QuikQuant_box-1024x683.jpg 1024w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a>The <span style=\"text-decoration: underline;\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=471000\">FMH QuikQuant Kit<\/a><\/span> is a flow cytometric method for FMH detection and quantification. The assay uses a reagent containing a monoclonal antibody to Hemoglobin F and Propidium Iodide as a specific marker of nucleated cells. The method is a rapid streamlined technique requiring about 30 minutes to complete. With less than 15 minutes of hands-on time needed, it is more efficient to use than the Kleihauer\u2013Betke assay, as well as more sensitive and precise.<\/p>\n<h4>Key Benefits<\/h4>\n<ul>\n<li>New technique is quicker to perform<\/li>\n<li>PI provides a definite tool for gating out the WBCs<\/li>\n<li>Kit includes 10X concentrated permeabilization reagent<\/li>\n<li>Works well with FETALtrol as a system control<\/li>\n<\/ul>\n<h4>Features<\/h4>\n<ul>\n<li>Detection of fetal hemoglobin (HbF) of as low as 0,06% fetal cells in maternal blood<\/li>\n<li>Results in 1 hour (20 minutes hands-on-time) with two single centrifuge steps<\/li>\n<li>IVD\/CE<\/li>\n<\/ul>\n<h4>Applications<\/h4>\n<ul>\n<li>Determination of Fetomaternal Hemorrhage<\/li>\n<li>Pregnancy with suspected RhD incompatibilities<\/li>\n<li>Abdominal trauma<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1380 size-full\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/FMH-QQ.png\" alt=\"FMH-QQ\" width=\"867\" height=\"288\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/FMH-QQ.png 867w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/FMH-QQ-300x100.png 300w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/FMH-QQ-768x255.png 768w\" sizes=\"auto, (max-width: 867px) 100vw, 867px\" \/><em>Figure 1: The figure above shows the results of the FMH QuikQuant . The red cells are the maternal RBCs, the blue cells are the maternal F- cells and the green cells are the HbF cells from the child.<\/em><\/p>\n<h4>Product Information<\/h4>\n<p><strong>FMH QuikQuant<\/strong><br \/>\n<strong>Product Code:<\/strong> <span style=\"text-decoration: underline;\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=471000\">QQF-100<\/a><\/span><br \/>\n<strong>Size:<\/strong> 100 Test Kit<br \/>\n<strong>Regulatory Status:<\/strong> CE\/IVD<\/p>\n<h4>Background Information<\/h4>\n<p>The most important clinical use of fetal RBC detection is the diagnosis and quantitation of fetomaternal hemorrhage (FMH).\u00a0 FMH occurs normally throughout pregnancy in minute amounts, with increasing volumes during the later stages of gestation [5].\u00a0 If there is a significant difference in the RBC antigenicity between the fetus and mother, this can result in allosensitization of the maternal immune system either before or after parturition.\u00a0 The maternal antibodies to the fetal RBC antigens may be clinically silent or cause life-threatening autoimmune sequelae for the current or subsequent pregnancies (e.g.: erythroblastosis fetalis or early abortion).\u00a0 Such sensitization can occur with any RBC antigen mismatch, but the highest frequency and profound clinical consequences occur with Rh or D-antigen mismatches.<\/p>\n<p>Detection and enumeration of Fetal RBCs is an essential part of the management of those patients with FMH treated with Rh immune globulin (RhIG) preparations.\u00a0 The use of Rh immune globulin prophylaxis is a universal practice, but dosing amounts and schedules have regional variations [7,8].\u00a0 Hence, the sensitivity and specificity of detection assays for FMH is a critical factor in therapeutic efficacy and subsequent clinical outcome.<\/p>\n<p>The most widely used assay for FMH detection has been the visual microscopic counting Kleihauer-Betke (KB) method, which is based upon the differences in solubility properties in acid conditions of fetal hemoglobin (HbF) from adult hemoglobin [9]\u00a0 While the KB method is easily performed by most clinical laboratories, it lacks sensitivity and exhibits poor reproducibility or precision (CVs of 50- 100%). Flow cytometric methods have been developed using the antigenic differences or quantitative assessment of fetal hemoglobin (HbF) to distinguish fetal RBCs from adult RBCs. These methods are more precise and less subjective. Nonetheless, many laboratories have continued to use the KB method due to the limited availability of Flow Cytometry.<\/p>\n<p>For more information, references, and FAQs, please visit:\u00a0<a href=\"http:\/\/www.iqproducts.nl\/fmh-quikquant\/\">http:\/\/www.iqproducts.nl\/fmh-quikquant\/<\/a><\/p>\n<p>The FMH Quikquant Kit from <span style=\"text-decoration: underline;\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/iq\/\">IQ Products<\/a><\/span> is distributed in the UK &amp; ROI by Caltag Medsystems. If you would like further information about any of their products, please contact us at <span style=\"text-decoration: underline;\"><a href=\"mailto:techsupport@caltagmedsystems.co.uk\">techsupport@caltagmedsystems.co.uk<\/a><\/span>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The FMH QuikQuant Kit is a flow cytometric method for FMH detection and quantification. The assay uses a reagent containing a monoclonal antibody to Hemoglobin F and Propidium Iodide as a specific marker of nucleated cells. The method is a<\/p>\n","protected":false},"author":13,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[13,17],"tags":[459,460],"class_list":["post-1378","post","type-post","status-publish","format-standard","hentry","category-clinical-diagnosis","category-flow-cytometry","tag-iq-products","tag-perinatal"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/1378","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=1378"}],"version-history":[{"count":10,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/1378\/revisions"}],"predecessor-version":[{"id":11563,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/1378\/revisions\/11563"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=1378"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=1378"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=1378"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}