{"id":1538,"date":"2016-07-26T16:12:35","date_gmt":"2016-07-26T16:12:35","guid":{"rendered":"http:\/\/www.caltagmedsystems.co.uk\/information\/?p=1538"},"modified":"2024-04-17T12:08:53","modified_gmt":"2024-04-17T12:08:53","slug":"haemoglobin-depletion-from-haemolysed-serum-and-plasma","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/haemoglobin-depletion-from-haemolysed-serum-and-plasma\/","title":{"rendered":"Haemoglobin Depletion From Haemolysed Serum and Plasma"},"content":{"rendered":"<p>Biotech Support Group bring you two alternate strategies for haemoglobin\u00a0removal from sample preparations\u00a0for proteomic &amp; genomic sample seperation and enrichment<\/p>\n<p><span style=\"text-decoration: underline;\"><strong><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/search_results.php?searchtype=all&amp;searchwords=hemoglobind\">Hemoglobind<\/a><\/strong><\/span>\u00a0\u2013 a binding strategy recommended for applications where haemoglobin is in relatively low abundance ie haemolysed serum.<\/p>\n<p><span style=\"text-decoration: underline;\"><strong><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/search_results.php?searchtype=all&amp;searchwords=hemovoid\">HemoVoid<\/a><\/strong><\/span> \u2013 a haemoglobin voiding strategy recommended for applications where haemoglobin is in relative high abundance ie erythrocyte lysates or whole blood.<\/p>\n<p>Plus trial kits:\u00a0<a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=788300\"><span style=\"text-decoration: underline;\"><strong>Hemoglobind Trial Kit<\/strong> <\/span><\/a>\u00a0and\u00a0<span style=\"text-decoration: underline;\"><strong><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=788301\">HemoTrial Kit<\/a>.<\/strong><\/span><\/p>\n<p>&nbsp;<\/p>\n<p><strong>The Hemoglobind\u2122<\/strong>\u00a0group of reagents for Haemoglobin Removal and Capture includes:<\/p>\n<ol>\n<li>HemogloBind\u2122 \u00a0-Hemoglobin Depletion From Hemolyzed Serum\/Plasma<\/li>\n<li>NuGel-HemogloBind\u2122 &#8211; Hemoglobin Capture Reagent From Blood and Hemolyzed Serum<\/li>\n<\/ol>\n<h4><strong>Hemoglobind\u2122<\/strong> &#8211; a solution for\u00a0Haemoglobin Removal and Capture.<\/h4>\n<p>Poly-electrolytes are polymers with repeating units of stationary charges. HemogloBind\u2122\u00a0comes from a class of solid-phase, or surface-based, elastomeric poly-electrolytic surfaces\u00a0that bind proteins through an empirically derived chemistry combining elements of polymer\u00a0composition, cross-linking architecture and charge properties. As with bio-polymers like\u00a0DNA and Heparin, governing their reactivity is the spatial presentation of the electrostatic\u00a0groups along a flexible polymer chain.<\/p>\n<p>Hemoglobind\u2122 has a high degree of specificity for hemoglobin, without cross-reacting with other\u00a0analytes. It can be used with the following samples to study analytical interferences, enzyme monitoring and blood substitutes:<\/p>\n<ul>\n<li>Haemolysed serum\/plasma<\/li>\n<li>Whole Blood or Red Cell lysates<\/li>\n<li>Tissue Homogenates<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/hemoglobind.png\" rel=\"attachment wp-att-1539\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1539 size-full\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/hemoglobind.png\" alt=\"hemoglobind\" width=\"565\" height=\"465\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/hemoglobind.png 565w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/hemoglobind-300x247.png 300w\" sizes=\"auto, (max-width: 565px) 100vw, 565px\" \/><\/a><\/p>\n<p>HemogloBind\u2122 does not cross react with most common serum components, making it an\u00a0excellent tool in numerous applications. These include analytical protocols where optical\u00a0interference is problematic, such as bilirubin analysis and bulk serum clarification.\u00a0Haemoglobin variants, as in thalassemia, bind with differential affinity towards\u00a0HemogloBind\u2122. For purification and\/or analysis of haemoglobin, a modest elevation in pH will\u00a0facilitate desorption of hemoglobin bound to HemogloBind\u2122.<\/p>\n<p><a href=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/hemoglobind2.png\" rel=\"attachment wp-att-1540\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-1540 size-full alignnone\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/hemoglobind2.png\" alt=\"hemoglobind2\" width=\"354\" height=\"394\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/hemoglobind2.png 354w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/hemoglobind2-270x300.png 270w\" sizes=\"auto, (max-width: 354px) 100vw, 354px\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<p><strong>Product Information<\/strong><\/p>\n<p><span style=\"text-decoration: underline;\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=788302\">Hemoglobind (5ml) &#8211; Product Code: H0145-05<br \/>\n<\/a><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=788303\">Hemoglobind (15ml) &#8211; Product Code: H0145-15<\/a><\/span><br \/>\n<span style=\"text-decoration: underline;\"> <a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=788304\">Hemoglobind (50ml) &#8211; Product Code: H0145-50<\/a><\/span><\/p>\n<p><span style=\"text-decoration: underline;\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=788300\">Hemoglobind Trial Kit \u00a0(5 mL Hemoglobind &amp; 5 preps of NuGel HemogloBind) &#8211; Product Code:\u00a0HB145K-05<\/a><\/span><\/p>\n<p><strong>Key Reference<\/strong><br \/>\nParvathi S. Kumar, et al. Peptide inhibitor of complement C1 modulates acute intravascular hemolysis of mismatched red blood cells in rats.\u00a0<span style=\"text-decoration: underline;\"><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27282513?utm_source=July+11+2016+&amp;utm_campaign=Biotech+Support+Group+BSG+Main+10142015&amp;utm_medium=email\">TRANSFUSION Volume 00, May 2016<\/a><\/span>.<\/p>\n<h4><\/h4>\n<h4><strong>NuGel-HemogloBind\u2122 <\/strong>&#8211; Hemoglobin Capture Reagent From Blood and Hemolyzed Serum<\/h4>\n<ul>\n<li>Has a high degree of specificity for hemoglobin binding up to 10 mg\/ml<\/li>\n<li>Removes hemoglobin from any species including human, mouse, rat, sheep, bovine, goat, etc<\/li>\n<li>Removes hemoglobin from organs, tissues.<\/li>\n<li>Hemoglobin removal from red blood cell lysate for proteomics and biomarker drug discovery<\/li>\n<li>The flow through fractions(hemoglobin depleted) retain their enzymatic and biological activity<\/li>\n<li>The flow through fractions(hemoglobin depleted) is compatible with LC-MS, activity based protein profiling and proteomic studies.<\/li>\n<li>Linearly scaleable up or down. Easy high throughput automation.<\/li>\n<\/ul>\n<p>NuGel-Hemoglobind\u2122 is reengineered for increased stability. It is based on NuGel\u2122 silica (50 microns in size, 1000\u00c5) covalently bound to elastomeric polyelectrolytes. It binds &gt;95% of hemoglobin from blood.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Product Information<\/strong><\/p>\n<p><span style=\"text-decoration: underline;\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=788315\">NuGel-HemogloBind\u2122 (25 preps). Product Code: NP-HO-T25<\/a><\/span><\/p>\n<p><span style=\"text-decoration: underline;\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=788316\">NuGel-HemogloBind\u2122 (50 preps). Product Code: NP-HO-T50<\/a><\/span><\/p>\n<p><span style=\"text-decoration: underline;\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=788300\">Hemoglobind Trial Kit \u00a0(5 mL Hemoglobind &amp; 5 preps of NuGel HemogloBind) &#8211; Product Code:\u00a0HB145K-05<\/a><\/span><\/p>\n<p>&nbsp;<\/p>\n<h4><strong>HemoVoid<\/strong> &#8211;\u00a0\u00a0Hemoglobin Depletion Reagent Kit<\/h4>\n<p>HemoVoid\u2122 , a silica-based protein enrichment matrix, removes hemoglobin from erythrocyte lysate samples while concentrating low abundance, and\/or low molecular weight proteins. The HemoVoid\u2122 protocol uses mild buffers; the protocol conditions are so gentle that native enzyme activity is retained in elution fractions. HemoVoid\u2122 derives from a silica-based library of individual mixed-mode ligand combinations (ionic, hydrophobic, aromatic, polymer). The library was designed to facilitate weak binding of proteins, allowing for rapid elution from the matrix without any foreknowledge of the variety of proteins contained in the starting sample. HemoVoid\u2122 depletes hemoglobin from red cell lysates while improving the resolution of less abundant blood proteins.<\/p>\n<ul>\n<li>Hemoglobin voids in flow-through &gt;98%, with &lt;30 minute bind\/wash\/elute protocol<\/li>\n<li>Hemoglobin removal from red cell lysates for RBC proteomics<\/li>\n<li>Hemoglobin removal from hemolyzed serum, blood and dried blood spot\/blood card<\/li>\n<li>Enrichment of hemoglobin variants<\/li>\n<li>Low abundance protein and enzyme enrichment<\/li>\n<li>Disposable, cost-effective and high-throughput<\/li>\n<li>Mild elution maintains tertiary structure and simple transfer to secondary analysis<\/li>\n<li>Removes hemoglobin from species including human, sheep, bovine, goat, etc.<\/li>\n<li>Removes hemoglobin from organs, tissues.<\/li>\n<li>The eluted fractions retain their enzymatic and biological activity<\/li>\n<li>The eluted fraction is compatible with LC-MS, activity based protein profiling and proteomic studies.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>Product Information<\/strong><\/p>\n<p><span style=\"text-decoration: underline;\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=788305\">HemoVoid\u2122 Kit (5 preps) Product Code: HVK-05<\/a><\/span><\/p>\n<p><span style=\"text-decoration: underline;\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=788306\">HemoVoid\u2122 Kit (10 preps) Product Code: HVK-10<\/a><\/span><\/p>\n<p><span style=\"text-decoration: underline;\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=788307\">HemoVoid\u2122 Kit (50 preps) Product Code: HVK-50<\/a><\/span><\/p>\n<p><span style=\"text-decoration: underline;\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=788308\">HemoVoid\u2122 Kit (100 preps) Product Code: HVK-100<\/a><\/span><\/p>\n<p>Caltag Medsystems are the UK distributor for Biotech Support Group &#8211; the manufacturers of Hemoglobind. For further information about their products, please contact us.<\/p>\n<p style=\"text-align: center;\"><a class=\"button_large\" href=\"mailto:techsupport@caltagmedsystems.co.uk\">Get In Touch<\/a><\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Biotech Support Group bring you two alternate strategies for haemoglobin\u00a0removal from sample preparations\u00a0for proteomic &amp; genomic sample seperation and enrichment Hemoglobind\u00a0\u2013 a binding strategy recommended for applications where haemoglobin is in relatively low abundance ie haemolysed serum. HemoVoid \u2013 a<\/p>\n","protected":false},"author":13,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[357],"class_list":["post-1538","post","type-post","status-publish","format-standard","hentry","category-general-information","tag-biotech-support-group"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/1538","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=1538"}],"version-history":[{"count":9,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/1538\/revisions"}],"predecessor-version":[{"id":2182,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/1538\/revisions\/2182"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=1538"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=1538"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=1538"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}