{"id":2248,"date":"2017-02-15T12:32:56","date_gmt":"2017-02-15T12:32:56","guid":{"rendered":"http:\/\/www.caltagmedsystems.co.uk\/information\/?p=2248"},"modified":"2017-02-15T12:55:02","modified_gmt":"2017-02-15T12:55:02","slug":"exocap-exosome-isolation-and-enrichment-kits","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/exocap-exosome-isolation-and-enrichment-kits\/","title":{"rendered":"ExoCap\u2122 Exosome Isolation and Enrichment Kits"},"content":{"rendered":"<p><em>Optimised for Serum, Plasma, and Cell Culture Supernatant.<\/em><\/p>\n<h5>What is ExoCap\u2122?<\/h5>\n<figure id=\"attachment_2238\" aria-describedby=\"caption-attachment-2238\" style=\"width: 300px\" class=\"wp-caption alignright\"><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/search_results.php?searchtype=all&amp;searchwords=exocap+capture+kit\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-2238 size-medium\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ExoCapProduct-300x172.jpg\" width=\"300\" height=\"172\" \/><\/a><figcaption id=\"caption-attachment-2238\" class=\"wp-caption-text\">ExoCap\u2122 Kits available now!<\/figcaption><\/figure>\n<p>ExoCap\u2122 is a kit for exosome isolation and enrichment designed and optimised for use with serum, plasma and cell culture supernatants. This kit consists of magnetic particles coupled with antibodies that recognise the exosome surface antigens (CD9, CD63, CD81, or EpCAM), treatment buffer, washing\/dilution buffer, and exosome elution buffer.<\/p>\n<p>ExoCap\u2122 uses functionalized Magnosphere\u2122 magnetic microparticles for exosome separation. These beads are coated with a JSR Life Sciences proprietary polymer and cofactors which specifically bind to exosomes.<\/p>\n<p style=\"text-align: center;\"><a class=\"button_large\" href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/search_results.php?searchtype=all&amp;searchwords=exocap+capture+kit\">View Products<\/a><\/p>\n<h5>Features and Advantages<\/h5>\n<ul>\n<li><strong>Simple<\/strong>: Simple workflow, easy-to-follow with minimum hands-on time,\u00a0protocol. Direct isolation possible, no front-end precipitation or ultracentrifugation needed.<\/li>\n<li><strong>Specific<\/strong>:\u00a0Targets specific antigens for purification of a defined exosome population.<\/li>\n<li><strong>Versatile<\/strong>:\u00a0Optimised for serum, plasma, and cell culture supernatant, with optimised protocols.<\/li>\n<li><strong>Intact<\/strong>:\u00a0Exosomes can be eluted intact preserving internal cargo.<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/exocap_protocol.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-2206\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/exocap_protocol.jpg\" alt=\"Exocap (MBL international) protocol shows capture and isolation of exosomes from Serum, plasma, and cell culture supernatant. Suitable for use in protein analusis, elute for intact exosomes, nucleic acid analysis\" width=\"1306\" height=\"622\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/exocap_protocol.jpg 1306w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/exocap_protocol-300x143.jpg 300w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/exocap_protocol-768x366.jpg 768w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/exocap_protocol-1024x488.jpg 1024w\" sizes=\"auto, (max-width: 1306px) 100vw, 1306px\" \/><\/a><\/p>\n<h5>ExoCap\u2122 Comparison Data<\/h5>\n\n<table id=\"tablepress-25\" class=\"tablepress tablepress-id-25\">\n<thead>\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><\/td><th class=\"column-2\">ExoCap\u2122 Kit<\/th><th class=\"column-3\">Ultracentrifuge<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><strong>Method<\/strong><\/td><td class=\"column-2\">Highly specific immunoprecipitation<\/td><td class=\"column-3\">Non-specific enrichment by centrifugation<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><strong>Purity<\/strong><\/td><td class=\"column-2\">High<\/td><td class=\"column-3\">Low<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><strong>Samples<\/strong><\/td><td class=\"column-2\">Optimized for serum, plasma, and cell culture supernatant<\/td><td class=\"column-3\">Cell culture supernatant, serum, plasma, urine<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><strong>Equipment<\/strong><\/td><td class=\"column-2\">Low-cost magnetic tube stand<\/td><td class=\"column-3\">Expensive ultracentrifuge<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><strong>Minimum Sample Volume<\/strong><\/td><td class=\"column-2\">100 \u03bcL<\/td><td class=\"column-3\">> 1 mL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-25 from cache -->\n<h5>ExoCap\u2122 Performance Data<\/h5>\n<p>ExoCap\u2122 enables isolation and enrichment of exosomal proteins.<\/p>\n<figure id=\"attachment_2211\" aria-describedby=\"caption-attachment-2211\" style=\"width: 1283px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ExoCap-performance-data.png\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2211\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ExoCap-performance-data.png\" alt=\"Western blot of isolated exosomal proteins in Serum and Plasma. Comparing Ultracentrifugation, ExoCap CD9 kit, CD63 Kit, CD81 Kit, Composite kit, and EpCAM Kit\" width=\"1283\" height=\"469\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ExoCap-performance-data.png 1283w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ExoCap-performance-data-300x110.png 300w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ExoCap-performance-data-768x281.png 768w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ExoCap-performance-data-1024x374.png 1024w\" sizes=\"auto, (max-width: 1283px) 100vw, 1283px\" \/><\/a><figcaption id=\"caption-attachment-2211\" class=\"wp-caption-text\">Isolated exosomal proteins from serum and plasma samples. Comparing Ultracentrifugation, <a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=796745\">ExoCap\u2122 CD9 kit<\/a>, <a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=796746\">ExoCap\u2122 CD63 Kit<\/a>, <a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=796747\">ExoCap\u2122 CD81 Kit<\/a>, <a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=796748\">ExoCap\u2122 Composite kit<\/a>, and <a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=796749\">ExoCap\u2122 EpCAM Kit<\/a>.<\/figcaption><\/figure>\n<figure id=\"attachment_2216\" aria-describedby=\"caption-attachment-2216\" style=\"width: 471px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ExoCap-performance-data3.png\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-2216 size-full\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ExoCap-performance-data3.png\" alt=\"tighter size distribution characteristic of exosomes compared to precipitation methods\" width=\"471\" height=\"290\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ExoCap-performance-data3.png 471w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ExoCap-performance-data3-300x185.png 300w\" sizes=\"auto, (max-width: 471px) 100vw, 471px\" \/><\/a><figcaption id=\"caption-attachment-2216\" class=\"wp-caption-text\">Particles isolated by ExoCap\u2122 kits exhibit a tighter size distribution characteristic of exosomes compared to precipitation method.<\/figcaption><\/figure>\n<p><a href=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ExoCap-performance-data2.png\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-2215\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ExoCap-performance-data2.png\" alt=\"exosomes from serum of healthy donor isolated with ExoCap CD9 Kit\" width=\"382\" height=\"265\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ExoCap-performance-data2.png 382w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ExoCap-performance-data2-300x208.png 300w\" sizes=\"auto, (max-width: 382px) 100vw, 382px\" \/><\/a><\/p>\n<h5>ExoCap\u2122 Citations<\/h5>\n<ul>\n<li>Woo JR et al. The Role of Isolation Methods on a Nanoscale Surface Structure and Its Effect on the Size of Exosomes. J Circ Biomark. 5, 11 (2016)<\/li>\n<li>Heinzelman P et al. Nanoscale Extracellular Vesicle Analysis in Alzheimer&#8217;s Disease Diagnosis and Therapy. Int J Alzheimers Dis. 2016, 8053139 (2016)\uff08PMID\uff1a<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27213078\">27213078<\/a>)<\/li>\n<li>Freedman JE et al. Diverse human extracellular RNAs are widely detected in human plasma. Nat Commun. 7, 11106 (2016) \uff08PMID\uff1a <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27112789\">27112789<\/a>)<\/li>\n<li>Patel RS et al. Adipose-derived stem cells from lean and obese humans show depot specific differences in their stem cell markers, exosome contents and senescence: role of protein kinase C delta (PKC\u03b4) in adipose stem cell niche. Stem Cell Investig. 3, 2 (2016) \uff08PMID\uff1a27358894)<\/li>\n<li>Puig-Basagoiti F et al. Human Cathelicidin Compensates for the Role of Apolipoproteins in Hepatitis C Virus Infectious Particle Formation. J Virol. 90, 8464 (2016) \uff08PMID\uff1a<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27440892\">27440892<\/a>)<\/li>\n<\/ul>\n<h5>Companion Products<\/h5>\n<p><a href=\"http:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=733626\">ExoCap\u2122 Nucleic Acid Elution Buffer<\/a> is designed for use with ExoCap\u2122 exosome isolation and enrichment kits for downstream nucleic acid extraction.<\/p>\n<p><a href=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ExoCap-companion-products.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-2217\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ExoCap-companion-products.jpg\" alt=\"exosome capture and nucleic acid elution kits to remove small RNA and Large RNAs from Exosomes\" width=\"1556\" height=\"492\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ExoCap-companion-products.jpg 1556w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ExoCap-companion-products-300x95.jpg 300w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ExoCap-companion-products-768x243.jpg 768w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ExoCap-companion-products-1024x324.jpg 1024w\" sizes=\"auto, (max-width: 1556px) 100vw, 1556px\" \/><\/a><\/p>\n<p style=\"text-align: left;\">Read More about the ExoCap\u2122 range from MBL International below:<\/p>\n<ul>\n<li><a href=\"https:\/\/www.caltagmedsystems.co.uk\/information\/exosome-research\/\">Exosome Research Overview<\/a><\/li>\n<li><a href=\"https:\/\/www.caltagmedsystems.co.uk\/information\/exocap-streptavidin-kit\/\">ExoCap\u2122 Streptavidin Kit<\/a><\/li>\n<li><a href=\"https:\/\/www.caltagmedsystems.co.uk\/information\/monoclonal-antibodies-for-common-exosomal-markers\/\">Monoclonal Antibodies for Common Exosomal Markers<\/a><\/li>\n<\/ul>\n<p style=\"text-align: center;\"><a class=\"button_large\" href=\"http:\/\/www.caltagmedsystems.co.uk\/contact.php\">Any questions? Get in touch!<\/a><\/p>\n<p><a href=\"http:\/\/www.caltagmedsystems.co.uk\/mbl\/\">MBL International<\/a> products are exclusively distributed in the UK and Ireland by Caltag Medsystems Ltd.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Optimised for Serum, Plasma, and Cell Culture Supernatant. What is ExoCap\u2122? ExoCap\u2122 is a kit for exosome isolation and enrichment designed and optimised for use with serum, plasma and cell culture supernatants. This kit consists of magnetic particles coupled with<\/p>\n","protected":false},"author":13,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[213],"tags":[215,214,67],"class_list":["post-2248","post","type-post","status-publish","format-standard","hentry","category-exosomes","tag-exocap","tag-exosomes","tag-mbl-international"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/2248","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=2248"}],"version-history":[{"count":6,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/2248\/revisions"}],"predecessor-version":[{"id":2270,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/2248\/revisions\/2270"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=2248"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=2248"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=2248"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}