{"id":2284,"date":"2017-02-20T17:18:40","date_gmt":"2017-02-20T17:18:40","guid":{"rendered":"http:\/\/www.caltagmedsystems.co.uk\/information\/?p=2284"},"modified":"2024-04-17T12:07:35","modified_gmt":"2024-04-17T12:07:35","slug":"nanoculture-plate","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/nanoculture-plate\/","title":{"rendered":"NanoCulture Plate"},"content":{"rendered":"

MBL International<\/a>, together with ORGANOGENIX (formerly SCIVAX Life Sciences), now offers the NanoCulture Plate<\/a> for 3D cell culturing. The NanoCulture Plate\/Dish (NCP\/NCD) is engineered with an evenly patterned nano-scale structure on a propietary plastic film attached to the bottom of the plate\/dish. The pattern mimics the extracellular matrix and allows cells to migrate across the plate and form healthy spheroids.<\/p>\n

In addition, the pattern eliminates the need for any kind of biological\/gel or matrix, allows for easy handling, and is fully compatible with lab apparatus, imaging apparatus, and high throughput screening systems. The plate is also compatible for drug screening, co-culture assay, EMT assay, hepatotoxicity assay, ADME assay, primary cell culture, stem cell culture and differentiation.<\/p>\n

It is well known that scaffold is very important for 3D cell culture to mimic tissue micro environment. However, working with gels are always a cumbersome procedure in terms of HTS or any kind of assay development. Therefore, researchers can use our plate instead of a gel platform and receive better biological relevance data compared to other plates, because the cells are free to move on NCP and forming spheroids. As a result, no gravity force or external materials are needed.<\/p>\n

View Products<\/a><\/p>\n

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NanoCulture Technologies for 3D Cell Culture <\/strong>\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0<\/strong><\/p>\n

Totally gel-free but scaffold-type 3D cell culture method – Micro-structure acts like a scaffold for cells<\/strong><\/p>\n

NanoCulture Plate\/Dish (NCP\/NCD) is engineered with an evenly patterned nano-scale structure on a proprietary plastic film which is the plate bottom surface. This pattern mimics the extracellular matrix and supports the cell to form cell spheroids. \u00a0This nano-scale structure controls the adhesion of cells to the film, and enhances cell migration and proliferation while cells form spheroids.<\/p>\n

\"NC1\"<\/p>\n

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Nano-scale patterned well-bottom film \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 <\/strong><\/p>\n

Structural color is observed, due to the interference of light by nano-scale pattern.<\/p>\n

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\"NC2\"<\/p>\n

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HeLa cell spheroid attaching to the nano-scale structure of film \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0<\/strong><\/p>\n

Several cells constituting a spheroid, contact the well-bottom film, \u00a0grip and \u00a0attach to nano-scale pattern using pseudopodium. \u00a0Data provided \u00a0by: Prof. \u00a0Hideo Namiki, Waseda UniversitySpheroid formation mechanism on NanoCulture Plate\/Dish (NCP\/NCD).<\/p>\n

\"NC3\"<\/p>\n

Cells seeded on a NCP\/NCD, grip and attach to the nano-scale pattern on a culture surface. \u00a0Cells start migrating randomly and dynamically as they do not adhere strongly to the base film thanks to the micro-structure, unlike conventional monolayer cell culture. \u00a0Cells contact and adhere to each other while migrating on the surface, \u00a0and \u00a0form spheroids, accompanied by proliferation at the same time. \u00a0Spheroid growth reaches a plateau as the culture period \u00a0progresses.<\/p>\n

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\"nc4\"<\/p>\n

\"nc5\"<\/p>\n

Chronological change of spheroid size of HepG2 cells<\/strong><\/p>\n

Box plot of spheroid diameter obtained from HepG2 cells cultured for 7 days on NCP (96 well plate) at 10,000 cells\/well. Spheroids keep growing as culture period progresses, while it reaches to a plateau in approximately one week.<\/p>\n

Format\u00a0& Advantages of NanoCulture Series<\/strong><\/p>\n

Assay-friendly well-sizes, optimized micro-structure pattern, and cell adhesive.<\/p>\n

NCP and NCD are available\u00a0as a 24, 96, 384-well plate or a 35 mm\u03a6 dish. \u00a0A variety of well formats is applicable for different experiments, from HTS to large scale preparation of spheroids.<\/p>\n

\"nc6\"<\/p>\n

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NCP (24, 96, 384 well) and NCD (35mm\u03a6)<\/p>\n

\"nc7\"<\/p>\n

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Structure of culture surface –\u00a0Left: Microsquare (MS), Right: Microhoneycomb (MH)<\/p>\n

\"nc7\"<\/p>\n

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There are two patterned micro-structures (Microsquare\/Microhoneycomb) and two types of cell adhesive in bottom film. We have evaluated more than 80 cell lines, and confirmed of pattern “preference” with approximately 10% of the cell types. \u00a0(These results are exhibited in our website. For more information, please contact us<\/a>, or test both patterns of NCP\/NCD with your cell types).<\/p>\n

Advantages\u00a0of NanoCulture Plate\/Dish<\/strong><\/p>\n

Easy to Use<\/strong>\u00a0> Same as conventional monolayer culture plates. After opening the package, just add cell suspension into wells.<\/p>\n

Good Microscopic Observation<\/strong>\u00a0> Capable of parfocal observation for all spheroids within the view field. Fully compatible with HTS imagers, confocal and convention<\/p>\n

Reproducibility<\/b>\u00a0> Coating reagents free. No biological materials. Made of plastic material, with almost no lot-to-lot variation.<\/p>\n

High Cell Viability<\/strong>\u00a0> Nano-scale scaffold allows natural migration and formation of spheroids resulting in high proliferation compared to \u00a0scaffold free 3D platforms.<\/p>\n

Natural Spheroid Formation<\/strong>\u00a0> Scaffold system eliminates the need for any external forces in spheroid formation.<\/p>\n

Large Scale Preparation of Spheroids<\/strong>\u00a0> 35mm\u03a6 dish with the same technology is available. \u00a0It is suitable for large scale 3D cell culture.<\/p>\n

Comparative Table of NCP\/NCD and Other 3D Cell Culture Methods<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n
<\/td>\nNCP\/NCD<\/td>\nScaffold<\/td>\nScaffold-Free<\/td>\n<\/tr>\n
Easy Handling<\/td>\n+++<\/strong><\/td>\n–<\/strong><\/td>\n\u00a0++<\/strong><\/td>\n<\/tr>\n
Excellent Observation(time-lapse imaging) (fluorescent imaging)<\/td>\n+++<\/strong><\/td>\n+<\/strong><\/td>\n\u00a0+<\/strong><\/td>\n<\/tr>\n
Uniform Manufacturing Lot<\/td>\n+++<\/strong><\/td>\n–<\/strong><\/td>\n\u00a0+++<\/strong><\/td>\n<\/tr>\n
High Cell Viablility after 1-week culture<\/td>\n+++<\/strong><\/td>\n+++<\/strong><\/td>\n\u00a0+<\/strong><\/td>\n<\/tr>\n
Fast Cell Growth<\/td>\n+++<\/strong><\/td>\n+++<\/strong><\/td>\n\u00a0+<\/strong><\/td>\n<\/tr>\n
Fast Spheroid Formation<\/td>\n+++<\/strong><\/td>\n+<\/strong><\/td>\n\u00a0+++<\/strong><\/td>\n<\/tr>\n
Wide Variation for Well Sizes(from mass culture to HTS)<\/td>\n+++<\/strong><\/td>\n++<\/strong><\/td>\n\u00a0–<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Spheroid morphology on NanoCulture Plate\/Dish<\/strong><\/p>\n

\"nc8\"<\/span><\/p>\n

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Spheroid morphology is totally depending on cell characteristics (migration, proliferation, intracellular adhesion molecules expression, or degree of differentiation). Typically, it can be classified into 4 types.<\/p>\n

Spheroid culture medium (M-type\/R-type) and Medium Supplement<\/strong><\/p>\n

Though cells can form spheroids in NCP with standard growth medium, we provide two types of culture medium. \u00a0NanoCulture Medium (NCM)<\/a> is optimised for spheroid formation, in combination with lot-checked serum. \u00a0In case of insufficient spheroid growth with M-type medium, it may improve by using R-type medium<\/a>. \u00a0We can also supply medium supplement, NanoCulture Medium Supplement (10x). \u00a0FBS is not included in this product, as you can prepare a spheroid culture medium with your own FBS.<\/p>\n

Spheroid Lysis Buffer and Spheroid Dispersing Solution<\/strong><\/p>\n

Spheroid Lysis Buffer (SLB)<\/a> dissolves spheroids into Lysate. \u00a0It can be used for DNA quantification for viablility measuring. \u00a0Spheroid Dispersion Solution (SD4x)<\/a> enzymatically disperses spheroids to single cell. \u00a0The cultured single cells can be used for cell counting, flow cytometry and subculture, etc.<\/p>\n

\"spher1\"<\/p>\n

Tumour Organoid Culture Kit<\/strong><\/a><\/p>\n

The need to harvest primary cancer cells from tumour in a 3D system arises from our desire to understand the patient specific tumour biology. \u00a0It is widely accepted that data obtained from primary cells is not only preferred, but most relevant when trying to study physiological interactions. \u00a0By culturing primary cancer cells on NCP, stromal cells do not cause overgrowth, but form co-cultured spheroids with cancer cells. \u00a0This kit is enabled to test and evaluate anti-cancer drug sensitivity from a small amount of specimen (>300 mg). The kit consists of three major components. \u00a01) Primary Culture Medium, containing growth factor supplements, 2) Dissociation Solution and tools, and 3) NanoCulture Plate.<\/p>\n

Caltag Medsystems are the UK distributor for MBL International products. Please contact our technical support team to discuss your requirements further.<\/p>\n

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Discuss Requirements<\/a><\/p>\n

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MBL International, together with ORGANOGENIX (formerly SCIVAX Life Sciences), now offers the NanoCulture Plate for 3D cell culturing. The NanoCulture Plate\/Dish (NCP\/NCD) is engineered with an evenly patterned nano-scale structure on a propietary plastic film attached to the bottom of<\/p>\n","protected":false},"author":13,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18],"tags":[67],"class_list":["post-2284","post","type-post","status-publish","format-standard","hentry","category-cell-culture","tag-mbl-international"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/2284","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=2284"}],"version-history":[{"count":19,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/2284\/revisions"}],"predecessor-version":[{"id":2303,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/2284\/revisions\/2303"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=2284"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=2284"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=2284"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}