{"id":3061,"date":"2017-11-16T16:37:27","date_gmt":"2017-11-16T16:37:27","guid":{"rendered":"http:\/\/www.caltagmedsystems.co.uk\/information\/?p=3061"},"modified":"2020-03-17T15:33:15","modified_gmt":"2020-03-17T15:33:15","slug":"measure-immune-response-treatment-efficacy-with-diaclone-elispot-kits","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/measure-immune-response-treatment-efficacy-with-diaclone-elispot-kits\/","title":{"rendered":"Measure Immune Response &#038; Treatment Efficacy with Diaclone ELISpot Kits"},"content":{"rendered":"<p><a href=\"https:\/\/www.caltagmedsystems.co.uk\/diaclone\/\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-3062\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/Diaclone_ELISpot_banner.png\" alt=\"highly specific immunoassay for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level \" width=\"620\" height=\"98\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/Diaclone_ELISpot_banner.png 620w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/Diaclone_ELISpot_banner-300x47.png 300w\" sizes=\"auto, (max-width: 620px) 100vw, 620px\" \/><\/a><\/p>\n<p>Monitoring the immune responses of patients treated with immunotherapy is essential to evaluate the efficacy of treatment and correlate clinical responses to Cytotoxic T Lymphocyte responses. <a href=\"https:\/\/www.caltagmedsystems.co.uk\/diaclone\/?ddid=2#dd2\">Diaclone ELISPot assays<\/a> are specific, accurate, sensitive, precise &amp; robust. This bioassay is recognised as the reference method to evaluate vaccine efficacy in clinical trials and has an expanding role in many immune related research &amp; clinical fields.<\/p>\n<ul>\n<li><a href=\"https:\/\/www.caltagmedsystems.co.uk\/diaclone\/?ddid=2#dd2\">Find out More<\/a><\/li>\n<li><a href=\"http:\/\/www.web-mailing.co.uk\/mailer\/lt.php?c=1771&amp;m=1653&amp;nl=650&amp;s=2d09aa5decba5414c8048090e7956b7e&amp;lid=27046&amp;l=-https--www.caltagmedsystems.co.uk\/pricing_ordering\/search_results.php--Q-searchwords--E-diaclone--A-MF_ID--E-0--A-CO_ID--E-0--A-HT_ID--E-0--A-AT_ID--E-0--A-ST_ID--E-0--A-PG_ID--E-91\">View ELISpot Products<\/a><\/li>\n<li>Download Application Notes: \u201c<a href=\"https:\/\/www.caltagmedsystems.co.uk\/docs\/Elispot_2014.pdf\">Diaclone ELISpot: monitoring the T-Cell response to tumour antigens<\/a>\u201d<\/li>\n<li><a href=\"https:\/\/www.caltagmedsystems.co.uk\/downloads\/Diaclone_ELISpot.pdf\">Download the Diaclone ELISpot Brochure<\/a><\/li>\n<\/ul>\n<h2>Why use Diaclone ELISpots?<\/h2>\n<ul>\n<li>Measure immune response &amp; treatment efficacy<\/li>\n<li>Quantify frequency of cytokine producing cells<\/li>\n<li>Highly sensitive detection level (1 cell in 100,000)<\/li>\n<li>Results at single cell level<\/li>\n<li>Measure antigen specific reactivity<\/li>\n<li>Respected, Reliable, Flexible<\/li>\n<li>Custom manufacture &amp; assay development available<\/li>\n<\/ul>\n<h2>Immunoassay Meets Bioassay<\/h2>\n<p><a href=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ELISpot-image.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignright size-medium wp-image-3184\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ELISpot-image-300x168.jpg\" alt=\"Immunoassay meets bioassay. ELISpot Image from Diaclone\" width=\"300\" height=\"168\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ELISpot-image-300x168.jpg 300w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ELISpot-image.jpg 633w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a>The ELISpot is a combination of both an immunoassay and bioassay. Living cells are cultured directly into the wells of the ELISpot plate, cells are stimulated and secreted protein captured and detected by sandwich immuno-enzyme technology. Diaclone ELISpot assays can detect both secreted cytokines and measure the frequency of single cells that simultaneously produce multiple cytokines or other effector molecules.<\/p>\n<p>Over 500 ELISpot products are available for apoptosis targets, cytokines, growth factors, cytotoxic molecules, making Diaclone\u2019s kits applicable for the several research &amp; clinical applications such as vaccine development, Cancer, Allergy, Autoimmune Disease, and Transplantation.<\/p>\n<h2>Diaclone ELISpot<\/h2>\n<p><a href=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ELISpot-kit.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignright size-medium wp-image-3185\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ELISpot-kit-300x168.jpg\" alt=\"ELISpot kit from Diaclone\" width=\"300\" height=\"168\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ELISpot-kit-300x168.jpg 300w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/ELISpot-kit.jpg 633w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a>Diaclone have multiple product formats to meet your needs<\/p>\n<ul>\n<li>ELISpot Kits &#8211; Pre coated plates &amp; reagents<\/li>\n<li>ELISpot Sets &#8211; Capture &amp; detection antibodies &amp; reagents<\/li>\n<li>Dual ELISpot Sets &#8211; Enzymatic detection of two proteins<\/li>\n<li>Dual Fluorospot Sets &#8211; Fluorometric detection of two proteins<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.caltagmedsystems.co.uk\/diaclone\/?ddid=2#dd2\">Find out more<\/a> about the different ELISpot formats available, or <a href=\"mailto:techsupport@caltagmedsystems.co.uk\">contact us<\/a> to find the most suitable kit for you.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-3063\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/Diaclone_ELISpot_table.png\" alt=\"highly specific immunoassay for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level \" width=\"846\" height=\"375\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/Diaclone_ELISpot_table.png 846w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/Diaclone_ELISpot_table-300x133.png 300w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/Diaclone_ELISpot_table-768x340.png 768w\" sizes=\"auto, (max-width: 846px) 100vw, 846px\" \/><\/p>\n<p>Diaclone are committed to providing excellence in Monoclonal Antibody and Immunoassay development. Their products are distributed in the UK and Ireland by <a href=\"https:\/\/www.caltagmedsystems.co.uk\/index.php\">Caltag Medsystems<\/a>. If you have any questions about the products in this blog, please contact us at <a href=\"mailto:techsupport@caltagmedsystems.co.uk\">techsupport@caltagmedsystems.co.uk<\/a>, or call +44 (0)1280 827460.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Monitoring the immune responses of patients treated with immunotherapy is essential to evaluate the efficacy of treatment and correlate clinical responses to Cytotoxic T Lymphocyte responses. Diaclone ELISPot assays are specific, accurate, sensitive, precise &amp; robust. This bioassay is recognised<\/p>\n","protected":false},"author":13,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[427],"tags":[428,429,50],"class_list":["post-3061","post","type-post","status-publish","format-standard","hentry","category-elispot","tag-diaclone","tag-elispot","tag-immunology"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/3061","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=3061"}],"version-history":[{"count":8,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/3061\/revisions"}],"predecessor-version":[{"id":5806,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/3061\/revisions\/5806"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=3061"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=3061"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=3061"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}