{"id":3078,"date":"2017-12-06T13:41:53","date_gmt":"2017-12-06T13:41:53","guid":{"rendered":"http:\/\/www.caltagmedsystems.co.uk\/information\/?p=3078"},"modified":"2020-03-17T15:32:51","modified_gmt":"2020-03-17T15:32:51","slug":"tvt-comparison","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/tvt-comparison\/","title":{"rendered":"Inter-laboratory Comparison of the TransFix\/EDTA Vacuum Blood Collection Tube (TVT) with the 5 mL Cyto-Chex\u00ae BCT"},"content":{"rendered":"<p><a href=\"https:\/\/www.cytomark.co.uk\/index.php\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-3098 aligncenter\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/Cytomark_Colour_HighRes_Logo-300x131.png\" alt=\"Cytomark, division of caltag medsystems, manufacturer of TransFix for flow cytometry sample stabilisation\" width=\"228\" height=\"100\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/Cytomark_Colour_HighRes_Logo-300x131.png 300w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/Cytomark_Colour_HighRes_Logo-768x337.png 768w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/Cytomark_Colour_HighRes_Logo-1024x449.png 1024w\" sizes=\"auto, (max-width: 228px) 100vw, 228px\" \/><\/a>Cytomark, a division of Caltag Medsystems, recently presented a scientific poster at the ICCS2017 conference in collaboration with\u00a0the Royal Hallamshire Hospital (Sheffield, UK),\u00a0Royal Sussex County Hospital (Brighton, UK), and\u00a0Roswell Park Cancer Institute (Buffalo, NY, USA).<\/p>\n<h2 style=\"text-align: center;\">&#8220;Inter-laboratory Comparison of the TransFix\/EDTA Vacuum Blood Collection Tube (TVT) with the 5 mL Cyto-Chex\u00ae BCT&#8221;<\/h2>\n<p>This study aimed to assess the performance of the TransFix Vacuum Blood Collection Tube (TVT) and the Cyto-Chex BCT devices across three clinical FC sites for their shared intended purpose of stabilizing lymphocytes for immunophenotyping via flow cytometry for up to 14 days.<\/p>\n<ul>\n<li>The TVT and BCT exhibited good clinical agreement with the \u201cgold standard\u201d BD Vacutainer result with regard to lymphocyte absolute count recoveries over the 15 day testing period.<\/li>\n<li>There were improved performance characteristics, in relation to the cell marker auto-fluorescence for lymphocytes recognized as CD3- and cell debris, from samples that were stabilized by the TVT over the BCT.<\/li>\n<\/ul>\n<p style=\"text-align: center;\"><a class=\"button_large\" href=\"https:\/\/www.cytomark.co.uk\/downloads\/TransFix_TVT_BCT_comparison_poster_ICCS2017.pdf\">Download Poster<\/a><\/p>\n<p><a href=\"https:\/\/www.cytomark.co.uk\/downloads\/TransFix_TVT_BCT_comparison_poster_ICCS2017.pdf\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-3079 size-medium\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/TransFix_TVT_BCT_comparison_poster_ICCS2017-300x282.png\" alt=\"Cytomark poster: Inter-laboratory Comparison of the TransFix\/EDTA Vacuum Blood Collection Tube (TVT) with the 5 mL Cyto-Chex\u00ae BCT\" width=\"300\" height=\"282\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/TransFix_TVT_BCT_comparison_poster_ICCS2017-300x282.png 300w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/TransFix_TVT_BCT_comparison_poster_ICCS2017-768x723.png 768w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/TransFix_TVT_BCT_comparison_poster_ICCS2017.png 1007w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<h2>Background<\/h2>\n<p>The \u201cTBNK panel\u201d in flow cytometry (FC) immunophenotyping has become a staple of most clinical FC laboratories worldwide. The panel is used to identify and enumerate the lymphocyte subsets within patient blood samples and is typically used for immune monitoring individuals with HIV. In addition, the panel is used to evaluate unexpected changes in cell numbers, indicative of certain immunological diseases, and as a method of demonstrating the pharmacodynamic effects of drugs such as rituximab.<\/p>\n<p>The \u201cgold standard\u201d collection tube is the EDTA Vacutainer (BD Vacutainer), but requires that FC analysis be performed 6 hours, or a maximum of 48 hours from venipuncture to prevent cellular degradation from affecting the immunophenotypic profile. Blood samples \u02c348 hours old exhibit indistinguishable cell subsets and inaccurate absolute cell counts leading to erroneous clinical results. In these situations fresh samples need to be collected, incurring extra costs, inconvenience to the patients involved and delayed analysis. Indeed in locations that do not have the infrastructure to test such specimens, samples must be transported for clinical evaluation and subsequently destroyed if this deadline cannot be met. Furthermore, for multisite clinical trials the<br \/>\npreservation of patient samples is of utmost importance in order to achieve reliable results when varying transit times are unavoidable.<\/p>\n<p>For these reasons, manufacturers Caltag Medsystems (Buckingham, UK) and Streck (Omaha, NE, USA) have developed the TransFix Vacuum Blood Collection Tube (TVT) and BCT, respectively to preserve the integrity of such critical specimens.<\/p>\n<h2>TransFix\/EDTA Vacuum Blood Collection Tubes<\/h2>\n<figure id=\"attachment_3074\" aria-describedby=\"caption-attachment-3074\" style=\"width: 300px\" class=\"wp-caption alignright\"><a href=\"https:\/\/www.cytomark.co.uk\/catalog\/index.php?CG_ID=1&amp;cid=0#cs1\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-3074 size-medium\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/TransFix-EDTA_Vacuum_Blood_Colection_Tubes_3ml_Tubes_IVD-300x219.jpg\" alt=\"Cytomark 3ml TransFix\/EDTA Vacuum Blood Collection Tubes for sample stabilisation for flow cytometry\" width=\"300\" height=\"219\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/TransFix-EDTA_Vacuum_Blood_Colection_Tubes_3ml_Tubes_IVD-300x219.jpg 300w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/TransFix-EDTA_Vacuum_Blood_Colection_Tubes_3ml_Tubes_IVD-768x561.jpg 768w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/TransFix-EDTA_Vacuum_Blood_Colection_Tubes_3ml_Tubes_IVD-1024x749.jpg 1024w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><figcaption id=\"caption-attachment-3074\" class=\"wp-caption-text\">3ml TransFix\/EDTA Vacuum Blood Collection Tubes<\/figcaption><\/figure>\n<p><a href=\"https:\/\/www.cytomark.co.uk\/catalog\/index.php?CG_ID=1&amp;cid=0#cs1\">TransFix\/EDTA Vacuum Blood Collection Tubes (TVT)<\/a> are manufactured in the UK by Cytomark, a division of Caltag Medsystems Ltd. and are available globally through a series of <a href=\"https:\/\/www.cytomark.co.uk\/distributors.php\">international distributors<\/a>.<\/p>\n<p>If you have any questions about these products, please <a href=\"mailto:cytomark@caltagmedsystems.co.uk\">contact us<\/a>, or <a href=\"https:\/\/www.cytomark.co.uk\/transfix_human_blood_stabilisation.php\">visit our website<\/a>.<\/p>\n<p><a href=\"https:\/\/www.cytomark.co.uk\/transfix_human_blood_stabilisation.php\">Further Information on Blood Stabilisation<\/a><\/p>\n<p><a href=\"https:\/\/www.cytomark.co.uk\/bibliography.php?bibliography_category=1\">Publications<\/a><\/p>\n<p><a href=\"https:\/\/www.caltagmedsystems.co.uk\/information\/reduce-variability-in-clinical-trials-by-stabilizing-samples\/\">View Related Blog<\/a><\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Cytomark, a division of Caltag Medsystems, recently presented a scientific poster at the ICCS2017 conference in collaboration with\u00a0the Royal Hallamshire Hospital (Sheffield, UK),\u00a0Royal Sussex County Hospital (Brighton, UK), and\u00a0Roswell Park Cancer Institute (Buffalo, NY, USA). &#8220;Inter-laboratory Comparison of the TransFix\/EDTA<\/p>\n","protected":false},"author":13,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[26],"tags":[201,430,431,432],"class_list":["post-3078","post","type-post","status-publish","format-standard","hentry","category-immunophenotyping","tag-clinical-flow-cytometry","tag-cytomark","tag-transfix","tag-tvts"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/3078","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=3078"}],"version-history":[{"count":17,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/3078\/revisions"}],"predecessor-version":[{"id":5804,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/3078\/revisions\/5804"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=3078"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=3078"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=3078"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}