{"id":388,"date":"2015-10-26T14:57:55","date_gmt":"2015-10-26T14:57:55","guid":{"rendered":"http:\/\/caltagmedsystems.co.uk\/information\/?p=388"},"modified":"2023-07-04T14:40:11","modified_gmt":"2023-07-04T14:40:11","slug":"double-keratinocyte-isolation-efficiency-with-isoboost","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/double-keratinocyte-isolation-efficiency-with-isoboost\/","title":{"rendered":"Double Keratinocyte Isolation Efficiency with Isoboost"},"content":{"rendered":"<p>Isolation is\u00a0a considerable stress for primary cells, in which the physical detachment and loss of cohesion results in significant loss of viability and reduced attachment.<img loading=\"lazy\" decoding=\"async\" class=\"alignright\" src=\"http:\/\/cellntec.com\/wp-content\/uploads\/jpg\/IsoBoost_count.jpg\" alt=\"\" width=\"242\" height=\"216\" \/><\/p>\n<p>The new IsoBoost supplement (<a href=\"https:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=733824&amp;group_1=All&amp;group_2=All&amp;supplier=All&amp;clonality=All&amp;host=All&amp;species=All&amp;applications=All&amp;regulatory_status=All&amp;searchwords=CnT-ISO-50\">CnT-ISO-50<\/a>) is able to significantly boost the isolation efficiency of primary human epithelial cells by protecting them against stress, and improving viability and attachment rates.<\/p>\n<p>In tests with the CnT-PR medium (left), more than twice as many population doublings were achieved in the first 7 days of culture when the <a href=\"https:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/product_detail.php?CI_ID=733824&amp;group_1=All&amp;group_2=All&amp;supplier=All&amp;clonality=All&amp;host=All&amp;species=All&amp;applications=All&amp;regulatory_status=All&amp;searchwords=CnT-ISO-50\">CnT-ISO-50<\/a> supplement was added during the first 3 days of culture. This resulted in 5x more cells than in standard medium alone:<\/p>\n<p><a class=\"notion-link-token notion-focusable-token notion-enable-hover\" tabindex=\"0\" href=\"https:\/\/www.caltagmedsystems.co.uk\/\" rel=\"noopener noreferrer\" data-token-index=\"0\"><span class=\"link-annotation-unknown-block-id--1999865189\">Caltag Medsystems <\/span><\/a>is the distributor of <a href=\"https:\/\/www.caltagmedsystems.co.uk\/cellntec\/\">CellnTech<\/a> products in the UK and Ireland. If you have any questions about these products, please <a class=\"notion-link-token notion-focusable-token notion-enable-hover\" tabindex=\"0\" href=\"https:\/\/www.caltagmedsystems.co.uk\/contact.php\" rel=\"noopener noreferrer\" data-token-index=\"5\"><span class=\"link-annotation-unknown-block-id--1821305553\">contact<\/span><\/a> <a class=\"notion-link-token notion-focusable-token notion-enable-hover\" tabindex=\"0\" href=\"https:\/\/www.caltagmedsystems.co.uk\/contact.php\" rel=\"noopener noreferrer\" data-token-index=\"7\"><span class=\"link-annotation-unknown-block-id--1821305553\">us<\/span><\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Isolation is\u00a0a considerable stress for primary cells, in which the physical detachment and loss of cohesion results in significant loss of viability and reduced attachment. The new IsoBoost supplement (CnT-ISO-50) is able to significantly boost the isolation efficiency of primary<\/p>\n","protected":false},"author":13,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18],"tags":[],"class_list":["post-388","post","type-post","status-publish","format-standard","hentry","category-cell-culture"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/388","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=388"}],"version-history":[{"count":5,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/388\/revisions"}],"predecessor-version":[{"id":7542,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/388\/revisions\/7542"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=388"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=388"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=388"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}