{"id":4433,"date":"2019-03-01T17:23:51","date_gmt":"2019-03-01T17:23:51","guid":{"rendered":"http:\/\/www.caltagmedsystems.co.uk\/information\/?p=4433"},"modified":"2020-03-17T11:07:20","modified_gmt":"2020-03-17T11:07:20","slug":"diaclone-elispot-ex-vivo-analysis-of-protein-secretion","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/diaclone-elispot-ex-vivo-analysis-of-protein-secretion\/","title":{"rendered":"Diaclone ELISpot: Ex-vivo Analysis of Protein Secretion"},"content":{"rendered":"

\"\"<\/a>The Enzyme Linked Immunospot (ELISpot) Assay is a versatile and highly sensitive method for ex-vivo analysis of protein secretion post cell stimulation. Virtue of its high sensitivity (1 cell in 100,000) the ELISpot assay enables frequency analysis of activated cell populations or antigen-specific cell responses at the single cell level and therefore can measure the magnitude of specific T-Cell immunity.<\/p>\n

ELISpot methodology: when Immunoassay meets bioassay<\/h2>\n

The ELISpot is a combination of both an immunoassay and bioassay. Living cells are cultured directly into the wells of the ELISpot plate, cells are stimulated and secreted protein captured and detected by sandwich immune-enzyme technology. Diaclone ELISpot assays can detect both secreted cytokines and measure the frequency of single cells that simultaneously produce multiple cytokines or other effector molecules.<\/p>\n

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ELISpot Applications: measurement of immune outcomes and treatments efficacy<\/h2>\n

Monitoring the immune responses of patients treated with immunotherapy is essential to evaluate the efficacy of treatment and correlate clinical responses to Cytotoxic T Lymphocyte responses. ELISpot assays are specific, accurate, sensitive precise and robust. This Bioassay is now recognised as the reference method to evaluate vaccine efficacy in clinical trials and has an ever-expanding role in may immunology related research and clinical fields.<\/p>\n

\"Vaccine<\/p>\n

Diaclone ELISpot: respected, reliable & flexible<\/h2>\n

Respected<\/h3>\n