{"id":4688,"date":"2019-05-22T09:02:01","date_gmt":"2019-05-22T09:02:01","guid":{"rendered":"http:\/\/www.caltagmedsystems.co.uk\/information\/?p=4688"},"modified":"2024-04-17T12:00:09","modified_gmt":"2024-04-17T12:00:09","slug":"3helix-overview-and-applications","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/3helix-overview-and-applications\/","title":{"rendered":"3Helix Overview and Applications"},"content":{"rendered":"

Collagen is the most abundant protein in the human body. It is a critical component of almost all organs and tissues, providing the framework for cell attachment and growth. Collagen degradation regulates normal tissue remodelling which occurs during development, homeostasis and repair,[1]<\/sup>\u00a0whereas excessive collagen degradation is a\u00a0key feature of tissues in a variety of diseases, such as cancer, inflammation, and fibrosis.[2]<\/sup><\/p>\n

All types of collagen from all species share the triple helical protein structure, which is nearly exclusively found in\u00a0collagen.\u00a0After being cleaved by a collagenase, the collagen molecule\u00a0becomes thermally unstable at body temperature and the triple helix spontaneously denatures.<\/strong>[3,4,5]\u00a0<\/sup>It is also found that unfolding of the collagen triple helix occurs during mechanical injuries of connective tissues and is an important damage mechanism.[6]<\/sup><\/p>\n

<\/p>\n

The founders of 3Helix invented the\u00a0Collagen Hybridizing Peptide<\/strong>\u00a0(CHP)<\/strong>\u00a0that can specifically bind to unfolded collagen molecules, by forming the triple helix with the denatured collagen chains, in a fashion analogous to a primer binding to a melted DNA strand during PCR.[4]<\/sup>\u00a0Conjugated with a\u00a0fluorescent or biotin label, CHP is the first probe\u00a0of its kind to enable direct detection of unfolded collagen molecules in virtually any tissue that has been\u00a0subject to\u00a0mechanical damage,[6]<\/sup>\u00a0or\u00a0enzymatic remodelling\u00a0(whether physiologically regulated, or disease associated).[5]<\/sup><\/p>\n

Collagen is also one of the most widely used natural scaffold materials for\u00a0regenerative medicine. The process of harvesting native extracellular\u00a0matrices by removing cells from animal tissues (i.e.,\u00a0tissue decellularisation) may alter the collagen structure and negatively affect the mechanical property and regenerative capacity of the ECM materials. CHP enables assessment\u00a0of the structural integrity\u00a0of collagen matrices from the molecular level, and facilitates\u00a0optimisation of the decellularisation protocols.[7]<\/sup><\/p>\n

Additionally, CHP can be used in several biochemical assays, such as\u00a0in-gel Western blot,[8]<\/sup>\u00a0for identification and possibly quantification of collagen content in a biological sample.<\/p>\n

CHP versus conventional collagen characterisation methods<\/p>\n

\"table2\"<\/a><\/p>\n

View Products<\/strong><\/a><\/h5>\n
Reference<\/h5>\n
    \n
  1. Matrix metalloproteinases and the regulation of tissue remodeling.\u00a0Nature Reviews Molecular Cell Biology<\/em>\u00a0(2007) 8, 221-233.<\/li>\n
  2. Targeting and mimicking collagens via triple helical peptide assembly.\u00a0Current Opinion in Chemical Biology<\/em>\u00a0(2013)\u00a0more info<\/a><\/li>\n
  3. Collagenase unwinds triple-helical collagen prior to peptide bond hydrolysis.\u00a0EMBO Journal<\/em>(2004) 23, 3020-3030.<\/li>\n
  4. Targeting collagen strands by photo-triggered triple-helix hybridization.\u00a0Proceedings of the National Academy of Science of the United States of America<\/em>\u00a0(2012)\u00a0more info<\/a><\/li>\n
  5. In situ<\/em>\u00a0imaging of tissue remodeling with collagen hybridizing peptides.\u00a0ACS Nano<\/em>\u00a0(2017)\u00a0(open access)<\/em>\u00a0more info<\/a><\/li>\n
  6. Molecular level detection and localization of mechanical damage in collagen enabled by collagen hybridizing peptides.\u00a0Nature Communications\u00a0<\/em>(2017)\u00a0(open access)<\/em>\u00a0more info<\/a><\/li>\n
  7. Molecular assessment of collagen denaturation in decellularized tissues using a collagen hybridizing peptide.\u00a0Acta Biomaterialia\u00a0<\/em>(2017)\u00a0(open access)<\/em>\u00a0more info<\/a><\/li>\n
  8. Direct detection of collagenous proteins by fluorescently labeled collagen mimetic peptides.\u00a0Bioconjugate Chemistry\u00a0<\/em>(2013)\u00a0more info<\/a><\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    Collagen is the most abundant protein in the human body. It is a critical component of almost all organs and tissues, providing the framework for cell attachment and growth. Collagen degradation regulates normal tissue remodelling which occurs during development, homeostasis<\/p>\n","protected":false},"author":13,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[853],"class_list":["post-4688","post","type-post","status-publish","format-standard","hentry","category-general-information","tag-3helix"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/4688","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=4688"}],"version-history":[{"count":3,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/4688\/revisions"}],"predecessor-version":[{"id":7441,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/4688\/revisions\/7441"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=4688"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=4688"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=4688"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}