{"id":4902,"date":"2025-04-16T16:00:27","date_gmt":"2025-04-16T16:00:27","guid":{"rendered":"http:\/\/www.caltagmedsystems.co.uk\/information\/?p=4902"},"modified":"2025-04-16T16:01:15","modified_gmt":"2025-04-16T16:01:15","slug":"multimericligands-higher-activity-lower-endotoxin","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/multimericligands-higher-activity-lower-endotoxin\/","title":{"rendered":"MultimericLigands™ – Higher Activity – Lower Endotoxin"},"content":{"rendered":"

AdipoGen Life Sciences’ Multimeric Proteins are high activity constructs in which two trimeric TNFSF ligands are linked via the oligomeric \"\"<\/a>collagen domain of ACRP30 [ACRP30headless] and therefore mimic the membrane-bound forms of the proteins.<\/p>\n<\/p>\n

The Largest Panel of Ultrapotent Multimeric Ligands\u2122
Equivalent to MegaLigands!<\/h4>\n<\/p>\n

\"\"<\/a><\/p>\n

Endogenous TNF superfamily ligands are either active as membrane-form (e.g. FasL, TRAIL, CD40L, OX40L) or are secreted and activated through oligomerisation by the binding of proteoglycans at the surface of cells (e.g. APRIL). The oligomerisation is triggered
\u2022 by fusing the TNF superfamily ligands to the collagen domain of the protein ACRP30 (which itself has no functional activity) to form a hexameric structure and therefore creating \u201cMultimeric Proteins\u201d<\/strong>, or
\u2022 by adding a cross-linking antibody called \u201cTNF Ligands Enhancer\u201d<\/strong> (Prod. No. AG-35B-0001).<\/p>\n

Multimeric FasL\u2122 [MegaFasL\u2122]<\/h2>\n

FasL (human) (multimeric) (rec.)<\/a><\/h3>\n

AG-40B-0130-C010 – 10 \u03bcg
AG-40B-0130-3010 – 3 x 10 \u03bcg (MultiPack)<\/p>\n

\u2022 Multimeric FasL\u2122 very effectively simulates the natural membrane-assisted aggregation of FasL in vivo.<\/p>\n

Source<\/strong>: HEK 293 cells.
Sequence<\/strong>: Human FasL (aa 139-281) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG\u00ae-tag.
Specificity<\/strong>: Binds to human and mouse Fas.
Biological Activity<\/strong>: Induces apoptosis of human JurkatT cells at a concentration of <1ng\/ml.
Endotoxin Content<\/strong>: <0.02EU\/\u03bcg purified protein (LAL test; Lonza).<\/p>\n

Literature References<\/strong>:
\u2022 Two adjacent trimeric Fas ligands are required for Fassignaling and formation of a death-inducing signaling complex: N. Holler, et al.; Mol. Cell. Biol. 23, 1428 (2003)
\u2022 A Fas agonist induces high levels of apoptosisin haematological malignancies: P. Greaney, et al.; Leuk. Res. 30, 415 (2006) MultimericFasL<\/p>\n<\/p>\n

\"\"<\/a><\/p>\n

Figure<\/strong>: Oligomerisation of FasL (human) efficiently triggers Jurkat cell death.<\/p>\n

Method<\/strong>: Jurkat cells were treated O\/N with the indicated concentrations of FasL (human) (multimeric) (rec.) (AG-40B-0130), Fc (human):FasL (human) (rec.) (AG-40B-0132), FasL (human) (rec.) (AG-40B-0001) or FasL (human) (rec.) + Enhancer (AG-44B-0001) (2 fold-dilutions, first concentration of 1000ng\/ml). Cell death was quantified using PMS\/MTS. The oligomeric FasL recombinant proteins (FasL (human) (multimeric), Fc (human):FasL (human) and FasL (human) + Enhancer) kill Jurkat cells at IC50 <0.2ng\/ml.<\/p>\n

Multimeric CD40L\u2122<\/h2>\n

CD40L (human) (multimeric) (rec.)<\/a><\/p>\n

AG-40B-0010-C010 10 \u03bcg
AG-40B-0010-3010 MultiPack 3 x 10 \u03bcg<\/p>\n

\u2022 MultimericCD40L\u2122 very effectively mimics the natural membrane-assisted aggregation of CD40L. It is the most potent alternative to activate CD40. No enhancer is required.<\/p>\n

\u2022 MultimericCD40L\u2122 is a potent B cell activator.<\/p>\n

Literature References<\/strong>:
\u2022 IgG subclass switch capacity is low in switched and in IgM-only, but high in IgD+IgM+, post-germinal center (CD27+) human B cells: C. Werner-Favre, et al.; Eur. J. Immunol. 31<\/strong>, 243 (2001)<\/p>\n<\/p>\n

\"\"<\/a><\/p>\n

Figure<\/strong>: CD40L (human) (multimeric) (rec.) (Prod. No. AG-40B-0010) does not need an enhancer to induce B cells activation.<\/p>\n\n\n

CD40L (human) (multimeric) (rec.) (Biotin)<\/a><\/td>AG-40B-0010B-C010
AG-40B-0010B-3010<\/td>		10 \u03bcg
MultiPack: 3 x 10 \u03bcg<\/td><\/tr>
CD40L (mouse) (multimeric) (rec.)<\/a><\/td>AG-40B-0020-C010
AG-40B-0020-3010<\/td>		10 \u03bcg
MultiPack: 3 x 10 \u03bcg<\/td><\/tr>
CD40L (mouse) (multimeric) (rec.) (Biotin)<\/a><\/td>AG-40B-0020B-C010
AG-40B-0020B-3010<\/td>		10 \u03bcg
MultiPack: 3 x 10 \u03bcg<\/td><\/tr>
CD40L (rat) (multimeric) (rec.)<\/a><\/td>AG-40B-0107-C010
AG-40B-0107-3010<\/td>		10 \u03bcg
MultiPack: 3 x 10 \u03bcg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Full Panel of Multimeric Ligands\u2122<\/h2>\n\n\n\n
APRIL (human) (multimeric) (rec.)<\/a><\/td>AG-40B-0017-C010
AG-40B-0017-3010<\/td>		10 \u03bcg
MultiPack: 3 x 10 \u03bcg<\/td><\/tr>
APRIL (human) (H98) (multimeric) (rec.)<\/a><\/td>AG-40B-0088-C010
AG-40B-0088-3010<\/td>		10 \u03bcg
MultiPack: 3 x 10 \u03bcg<\/td><\/tr>
APRIL (mouse) (multimeric) (rec.)<\/a><\/td>AG-40B-0089-C010
AG-40B-0089-3010<\/td>		10 \u03bcg
MultiPack: 3 x 10 \u03bcg<\/td><\/tr>
APRIL (mouse) (H98) (multimeric) (rec.)<\/a><\/td>AG-40B-0035-C010
AG-40B-0035-3010<\/td>		10 \u03bcg
MultiPack: 3 x 10 \u03bcg<\/td><\/tr>
OX40L (mouse) (multimeric) (rec.)<\/a><\/td>AG-40B-0029-C010<\/td>10 \u03bcg<\/td><\/tr>
TNF-\u03b1 (human) (multimeric) (rec.)<\/a><\/td>AG-40B-0019-C010<\/td>10 \u03bcg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
\n
Read About Multimeric Ligands on Adipogen’s Website<\/strong><\/a><\/div>\n\n\n\n
View Multimeric Proteins Flyer<\/strong><\/a><\/div>\n<\/div>\n\n\n\n
<\/div>\n\n\n\n
\n\n\n\n

Originally posted by Adipogen on: https:\/\/adipogen.com\/multimeric-ligands<\/a><\/p>\n\n\n\n

Caltag Medsystems <\/a>is the distributor of Adipogen<\/a> products in the UK and Ireland. If you have any questions about these products, please contact<\/a> us<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"

AdipoGen Life Sciences’ Multimeric Proteins are high activity constructs in which two trimeric TNFSF ligands are linked via the oligomeric collagen domain of ACRP30 [ACRP30headless] and therefore mimic the membrane-bound forms of the proteins. The Largest Panel of Ultrapotent Multimeric<\/p>\n","protected":false},"author":13,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1,681],"tags":[279,1030],"class_list":["post-4902","post","type-post","status-publish","format-standard","hentry","category-general-information","category-proteins","tag-adipogen","tag-multimeric-ligands"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/4902","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=4902"}],"version-history":[{"count":10,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/4902\/revisions"}],"predecessor-version":[{"id":12098,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/4902\/revisions\/12098"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=4902"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=4902"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=4902"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}