{"id":5082,"date":"2019-07-25T10:43:39","date_gmt":"2019-07-25T10:43:39","guid":{"rendered":"http:\/\/www.caltagmedsystems.co.uk\/information\/?p=5082"},"modified":"2019-07-25T10:43:39","modified_gmt":"2019-07-25T10:43:39","slug":"chp-histopathology","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/chp-histopathology\/","title":{"rendered":"CHP Histopathology"},"content":{"rendered":"

After being degraded by specific enzymes, the triple helical collagen molecule becomes thermally unstable and spontaneously unfolds at body temperature, leaving denatured collagen fragments covalently crosslinked within the partially degraded matrix.[1,2]<\/sup>\u00a0Such denatured collagen is a hallmark indicator of inflammation and\u00a0tissue damage<\/em>\u00a0under many pathological conditions, as well as programmed\u00a0tissue remodeling<\/em>\u00a0during physiological and developmental events. 3Helix\u2019s\u00a0Collagen Hybridizing Peptide (CHP)<\/a>\u00a0is the\u00a0first\u00a0<\/em>and\u00a0only<\/em>\u00a0agent that specifically targets this biomarker in histopathology.[1]<\/sup>\u00a0This powerful tool may enable straightforward applications in disease diagnosis, staging, as well as facile evaluation of therapeutic candidates by histology. CHP is applicable to nearly all tissue types and a wide range of\u00a0medical areas, such as\u00a0myocardial infarction<\/strong>,\u00a0arthritis<\/strong>,\u00a0nephritis<\/strong>, and\u00a0fibrosis<\/strong>.<\/p>\n


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Pulmonary fibrosis.<\/strong>\u00a0Representative fluorescence micrographs of the subpleural areas of lung cryosections obtained from mice dosed with bleomycin through minipumps for varying time periods versus control mice dosed with PBS for 1 week, and stained with\u00a0F-CHP<\/a>\u00a0and Hoechst 33342.\u00a0Highly localized, bright F-CHP signals revealed\u00a0spotty distribution of damaged collagen appearing beginning 1 week after bleomycin treatment. The overall CHP signals increased as the disease progressed from week 1 to week 3 and persisted through week 4.[1]<\/sup><\/p>\n

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Myocardial infraction.<\/strong>\u00a0A fluorescence image of a mouse heart at day 7\u00a0post myocardial infraction (left), stained by biotin-labeled\u00a0B-CHP<\/a>\u00a0(detected with AlexaFluor647-streptavidin), in comparison to a normal heart (right). Intense signals resulting from CHP binding to the degraded and denatured collagen can be seen in the damaged myocardial tissue.[1]<\/sup><\/p>\n

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Osteoarthritis.\u00a0<\/strong>Micrographs of articular cartilage tissue within the osteoarthritic or normal region from an osteoarthritis patient,\u00a0stained by fluorescein-labeled\u00a0F-CHP<\/a>\u00a0(green) and Hoechst 33342 (blue), showing high levels of degraded\u00a0type II collagen in the degenerated\u00a0cartilage.[1]<\/sup>\u00a0<\/em><\/p>\n

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Glomerulonephritis.<\/strong>\u00a0Micrographs of kidney cryosections from anti-Thy-1 nephritic and normal rats stained with fluorescent\u00a0F-CHP<\/a>\u00a0(green) and an anti-collagen IV antibody (red). The F-CHP signal is confined to the damaged collagen in the inflamed glomeruli,[1]<\/sup>\u00a0whereas the collagen IV antibody visualizes all collagen IV content uniformly distributed in\u00a0the tissue.<\/p>\n

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Bone development.\u00a0<\/strong>Localization of CHP binding in sagittal cryosections of mouse embryos from day 14 to 18 post-coitum (E14-18) that were stained with fluorescein-labeled\u00a0F-CHP<\/a>\u00a0(orange\/yellow) and Hoechst 33342 (blue), clearly showing the collagen degradation detected by CHP was localized exclusively in the developing skeletal tissue during this endochondral ossification process,[1]<\/sup>\u00a0wherein\u00a0a pre-formed cartilage template is resorbed and replaced by mineralized bone.<\/p>\n

This example demonstrates that CHP can be used in the research of developmental biology of the skeletal system; it may\u00a0be particularly useful for studying genetic disorders or factors in skeletal development, and for characterizing animals with a broad spectrum of skeletal phenotypes (e.g., diversity outbred mice).<\/p>\n

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Skin aging.<\/strong>\u00a0Fluorescence micrographs of formalin-fixed paraffin-embedded skin sections from 21-day old\u00a0versus 9-month old mice, stained with Hoechst 33342 (blue) and biotin-labeled\u00a0B-CHP<\/a>\u00a0(detected by AlexaFluor647-streptavidin, yellow), displaying significantly greater\u00a0amount of degraded collagen in the dermis of the aged skin. Reprinted from ref [1] with permission. In addition to chronological skin aging, CHP may be used to detect collagen damage in photoaging, skin inflammation and infection, and autoimmune skin disorders, as well as to evaluate the effectiveness or safety of skin care products and ingredients.<\/p>\n

Applicable organs and tissues:<\/strong>blood vessels, heart, lung, liver, kidney, muscles, gastrointestinal tract, genital system, breast, bone, joints, soft tissues, cartilage, ligament, tendon, oral cavity, embryo, eyes, skin<\/p>\n

Potential disease and medical areas:<\/strong>atherosclerosis, giant cell arteritis, aneurysm, myocardial infarction, respiratory inflammation, pneumonia, asthma, pulmonary fibrosis, effects of air pollution, liver fibrosis, cirrhosis, glomerulonephritis, kidney fibrosis, inflammatory bowel disease, bladder inflammation, prostatitis, uterine fibroid, cervical cancer, mammary development, breast cancer, muscle disorders, muscular dystrophy, osteoporosis, bone fracture, intervertebral disc degeneration, spondylosis, osteoarthritis, rheumatoid arthritis, gout, lupus, scleroderma, orthopedics, sports medicine, rotator cuff tear\/disease, tendinosis, whiplash, ligament tear, trauma, oral inflammation, periodontitis, oral mucositis, skeletal development, genetic connective tissue disorders, marfan syndrome, collagen mutations, osteogenesis imperfecta, cornea injury & infection, LASIK, keratoconus, fuchs’ dystrophy, glioblastoma, photoaging, skin cancer, melanoma, skin disorder, dermatitis, rheumatic skin diseases, cosmetic dermatology, diabetic ulcer, cancer invasion and metastasis, foreign body response to implants & transplants, angiogenesis, wound healing, ulcers, adhesion<\/p>\n

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Reference<\/p>\n

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  1. In situ<\/em>\u00a0imaging of tissue remodeling with collagen hybridizing peptides.\u00a0ACS Nano<\/em><\/strong>\u00a0(2017)\u00a0(open access)\u00a0<\/em>more info<\/a><\/li>\n
  2. Targeting collagen strands by photo-triggered triple-helix hybridization.\u00a0Proceedings of the National Academy of Sciences of the United States of America\u00a0<\/strong><\/em>(2012)\u00a0more info<\/a><\/li>\n<\/ol>\n<\/div>\n<\/div>\n<\/div>\n
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    Product Citations<\/p>\n

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    1. Jeffery Molkentin\u00a0et al.<\/em>, An acute immune response underlies the benefit of cardiac adult stem cell therapy.\u00a0bioRxiv<\/em><\/strong>\u00a0(2019)\u00a0more info<\/a><\/li>\n
    2. Xudong Li\u00a0et al.<\/em>,\u00a0Molecular detection and assessment of intervertebral disc degeneration\u00a0via<\/em>\u00a0a collagen hybridizing peptide.\u00a0ACS Biomaterials Science & Engineering<\/em><\/strong>\u00a0(2019)\u00a0more info<\/a><\/li>\n
    3. Xudong Li\u00a0et al.<\/em>,\u00a0Microfluidic disc-on-a-chip device for mouse intervertebral disc\u2014pitching a next-generation research platform to study disc degeneration.\u00a0ACS Biomaterials Science & Engineering<\/em><\/strong>\u00a0(2019)\u00a0more info<\/a><\/li>\n
    4. Karl Kadler\u00a0et al<\/em>., Protection of circadian rhythms by the protein folding chaperone, BiP.\u00a0The FASEB Journal<\/em><\/strong>\u00a0(2019)\u00a0more info<\/a><\/li>\n
    5. Stephen Weiss\u00a0et al.<\/em>,\u00a0Divergent matrix-remodeling strategies distinguish developmental from neoplastic mammary epithelial cell invasion programs.\u00a0Developmental Cell<\/em><\/strong>\u00a0(2018)\u00a0more info<\/a><\/li>\n
    6. Fiona Watt\u00a0et al<\/em>., Fibroblast state switching orchestrates dermal maturation and wound healing.\u00a0Molecular Systems Biology<\/em><\/strong>\u00a0(2018)\u00a0more info<\/a><\/li>\n
    7. Fiona Watt\u00a0et al<\/em>., Loxl2 is dispensable for dermal development, homeostasis and tumour stroma formation.\u00a0Plos One<\/strong><\/em>\u00a0(2018)\u00a0more info<\/a><\/li>\n
    8. Matthew Abramowitz\u00a0et al.<\/em>,\u00a0Skeletal muscle fibrosis is associated with decreased muscle inflammation and weakness in patients with chronic kidney disease.\u00a0American Journal of Physiology-Renal Physiology<\/em><\/strong>\u00a0(2018)\u00a0more info<\/a><\/li>\n
    9. Wayne Knox &\u00a0Krystel Huxlin\u00a0et al.<\/em>,\u00a0A non-ablative technique for femtosecond laser-based refractive correction: development, efficacy, and tissue effects. (2018)\u00a0more info<\/a><\/li>\n
    10. Svenja Illien-Junger\u00a0et al.<\/em>, Dietary advanced glycation end-product consumption leads to mechanical stiffening of murine intervertebral discs.\u00a0Disease Models & Mechanisms<\/strong><\/em>\u00a0(2018)\u00a0more info<\/a><\/li>\n<\/ol>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

      After being degraded by specific enzymes, the triple helical collagen molecule becomes thermally unstable and spontaneously unfolds at body temperature, leaving denatured collagen fragments covalently crosslinked within the partially degraded matrix.[1,2]\u00a0Such denatured collagen is a hallmark indicator of inflammation and\u00a0tissue<\/p>\n","protected":false},"author":13,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-5082","post","type-post","status-publish","format-standard","hentry","category-general-information"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/5082","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=5082"}],"version-history":[{"count":22,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/5082\/revisions"}],"predecessor-version":[{"id":5112,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/5082\/revisions\/5112"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=5082"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=5082"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=5082"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}