{"id":5208,"date":"2024-09-03T15:15:49","date_gmt":"2024-09-03T15:15:49","guid":{"rendered":"http:\/\/www.caltagmedsystems.co.uk\/information\/?p=5208"},"modified":"2024-09-03T15:15:49","modified_gmt":"2024-09-03T15:15:49","slug":"cd1d","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/cd1d\/","title":{"rendered":"CD1d"},"content":{"rendered":"\n

CD1d molecules are non-polymorphic MHC class I-like proteins. These are a type of non-classical MHCs. They present phospholipid and glycosphingolipid antigens to invariant Natural Killer T (iNKT) cells, a subset of CD1d-restricted T cells. This axis is involved in the regulation of nearly all aspects of the innate and acquired immune responses.1<\/sup><\/sub> These iNKT cells bridge the innate and acquired immune pathways. On activation, these cells can assume a Th1, Th2 or Th17 immune functionality profile. They can then directly exhibit cytotoxicity. The CD1d-iNKT cell axis plays a significant role in the antimicrobial, antitumor and autoimmune response.1<\/sup><\/p>\n\n\n\n

The CD1d molecules are expressed on myeloid cells as well as lymphoid cells. They are also found on epithelial and vascular smooth muscle cells. These are regulated by multiple transcription factors including TFSP1 and LEF-1 in humans and members of the ETS family in mice.1<\/sup><\/p>\n\n\n\n

Lipid presentation by CD1d occurs at the surface. CD1d undergoes internalisation and trafficking to the cytosol in lysosomal and endosomal compartments from the cell surface. The CD1d molecules exchange their ligands with glycolipids and return to the surface. The glycolipids may be acquired from exogenous sources or endogenous to the cell. The lipids are presented at the surface by the CD1d molecules.1\u00a0Ligands that can be presented by CD1d molecules include bacterial glycolipids, glycolipids from a marine sponge \u03b1-galactosylceramide (\u03b1-GalCer), normal endogenous glycolipids and glycolipids and phospholipids derived from tumours. Some ligands are also non-lipidic molecules.2<\/sup>\u00a0CD1d loaded with \u03b1-GalCer can activate human and murine CD1d-restricted NKT cells.<\/p>\n\n\n\n

CD1d Tetramers<\/h2>\n\n\n\n

MBL International offers CD1d tetramers. These are prepared by tetramerisation of CD1d complexes and \u03b22m by PE- or APC-labelled streptavidin. Binding this reagent to \u03b1-GalCer enables the highly sensitive detection of CD1d-restricted NKT cells and can be combined with antibodies to study NKT cell function through flow cytometry.<\/p>\n\n\n\n

\u03b1-GalCer loaded and \u03b1-GalCer unloaded tetramers are available.<\/p>\n\n\n\n

Product<\/th>\u03b1-GalCer unloaded Product Code No.<\/th>\u03b1-GalCer loaded Product Code No.<\/th><\/tr><\/thead>
Human CD1d Tetramer-PE<\/td>MBL-TS-HCD-1<\/a><\/td>MBL-TS-HCG-1<\/a><\/td><\/tr>
Human CD1d Tetramer-APC<\/td>MBL-TS-HCD-2<\/a><\/td>MBL-TS-HCG-2<\/a><\/td><\/tr>
Mouse CD1d Tetramer-PE<\/td>MBL-TS-MCD-1<\/a><\/td>MBL-TS-MCG-1<\/a><\/td><\/tr>
Mouse CD1d Tetramer-APC<\/td>MBL-TS-MCD-2<\/a><\/td>MBL-TS-MCG-2<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Dissolving and loading \u03b1-GalCer on CD1d tetramer<\/h3>\n\n\n\n
\"\"<\/a><\/figure>\n\n\n\n

Experimental protocols:\u00a0Detection of human NKT cells with CD1d tetramer<\/h3>\n\n\n\n

PBMC were separated from the peripheral blood of healthy subjects and incubated at room temperature for 5 minutes with 40 ul of Clear Back (Human Fc receptor blocking reagent, MBL code no. MTG-001). CD3-FITC and human CD1d tetramer-PE (with or without binding of \u03b1-GalCer) were added and incubated for 30 minutes at 4\u00b0C protected from light, and cells were analysed by flow cytometry.<\/p>\n\n\n\n

\"\"<\/a><\/figure>\n\n\n\n

Results showed total cells contained 0.05% NKT cells, as defined by CD1d\/CD3 dual positivity.<\/p>\n\n\n\n

Research highlights<\/h3>\n\n\n\n