{"id":5364,"date":"2019-10-22T08:36:43","date_gmt":"2019-10-22T08:36:43","guid":{"rendered":"http:\/\/www.caltagmedsystems.co.uk\/information\/?p=5364"},"modified":"2022-03-10T13:51:47","modified_gmt":"2022-03-10T13:51:47","slug":"manganese-mn2-as-a-new-activator-of-the-nlrp3-inflammasome-in-the-brain","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/manganese-mn2-as-a-new-activator-of-the-nlrp3-inflammasome-in-the-brain\/","title":{"rendered":"Manganese (Mn2+) as a New Activator of the NLRP3 Inflammasome in the Brain"},"content":{"rendered":"<p>Inflammasomes are implicated in physiological and pathological inflammation including neurodegenerative disorders such Parkinson or Alzheimer diseases. Divalent manganese (Mn<sup>2+<\/sup>) activates chronic inflammation leading to increase of neurotoxicity. Recently, the lab of A.G. Kanthasamy (Iowa State University, US) showed that Mn<sup>2+<\/sup>\u00a0activates the inflammasome protein NLRP3 through mitochondrial dysfunction in microglial cells, consequently promoting neuroinflammation. Interestingly, it was observed in the same study that Mn<sup>2+<\/sup>\u00a0stimulates the release of the adaptor protein ASC in exosomes that can propagate inflammasome activation in neighboring cells.<\/p>\n<h4>Following standard inflammasome antibodies from AdipoGen Life Sciences are used in this study in western blot and immunocytochemistry applications:<\/h4>\n<p>&#8211;\u00a0anti-Caspase-1 (p20) (mouse), mAb (Casper-1) (Prod. No. <a href=\"https:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/search_results.php?searchtype=all&amp;searchwords=AG-20B-0042\">AG-20B-0042<\/a>)<\/p>\n<p>&#8211;\u00a0anti-NLRP3\/NALP3, mAb (Cryo-2) (Prod. No. <a href=\"https:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/search_results.php?searchtype=all&amp;searchwords=AG-20B-0014\">AG-20B-0014<\/a>)<\/p>\n<p>&#8211;\u00a0anti-Asc, pAb (AL177) (Prod. No. <a href=\"https:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/search_results.php?searchtype=all&amp;searchwords=AG-25B-0006\">AG-25B-0006<\/a>)<\/p>\n<p>LIT:\u00a0<em>Manganese activates NLRP3 inflammasome signaling and propagates exosomal release of ASC in microglial cells:\u00a0<a href=\"http:\/\/stke.sciencemag.org\/content\/12\/563\/eaat9900\" target=\"_blank\" rel=\"noopener noreferrer\">S. Sarkar, et al.; Science Signal. 12, 563 (2019)<\/a><\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Inflammasomes are implicated in physiological and pathological inflammation including neurodegenerative disorders such Parkinson or Alzheimer diseases. Divalent manganese (Mn2+) activates chronic inflammation leading to increase of neurotoxicity. Recently, the lab of A.G. Kanthasamy (Iowa State University, US) showed that Mn2+\u00a0activates<\/p>\n","protected":false},"author":13,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[279],"class_list":["post-5364","post","type-post","status-publish","format-standard","hentry","category-general-information","tag-adipogen"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/5364","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=5364"}],"version-history":[{"count":3,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/5364\/revisions"}],"predecessor-version":[{"id":5671,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/5364\/revisions\/5671"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=5364"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=5364"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=5364"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}