Uncultured Or Low Passage Human Chondrocytes<\/h4>\n
CELLvo™ Human Chondrocytes (<40y)<\/strong> are mature, primary cells\u00a0sourced from young individuals. They may be selected as uncultured (P0), or isolated and expanded on CELLvo™ Matrix.<\/p>\n CELLvo™\u00a0Healthy Human Chondrocytes (>50) <\/strong>are primary cells, sourced from healthy individuals 50 to 70-years old and verified free of osteoarthritis. Cells are supplied as uncultured (P0).<\/p>\n CELLvo™ Osteoarthritic Human Chondrocytes (>50) <\/strong>are primary cells, sourced from osteoarthritic individuals 50 to 70-years old. Cells are supplied as uncultured (P0).<\/p>\n<\/div>\n<\/div>\n CELLvo™ Matrix<\/strong> is an Extracellular Matrix providing unique support for chondrocyte expansion. When grown on this matrix, CELLvo™ Human Chondrocytes attach and proliferate more rapidly, and exhibit similar morphology to in vivo chondrocytes. CELLvo™ Matrix is composed of more than 150 proteins that were secreted and assembled by bone marrow cells. The final product is deposited on the surface of a culture vessel. Cells are then gently extracted leaving a a native three-dimensional microenvironment providing for rapid and reliable expansion of cells.<\/p>\n<\/div>\n<\/div>\n If you can\u2019t find an answer to your question, call us on 01280 827460<\/em> or send us an<\/em> e-mail<\/a>, <\/em>we\u2019re be happy to help!<\/em><\/p>\n Q: Are CELLvo™ Human Chondrocytes better than most commercially available Chondrocytes?<\/strong><\/p>\n Yes, CELLvo™ Human Chondrocytes are sourced from rigorously defined donor tissue specified by age and health status. Our\u00a0Human Chondrocytes are gently processed and provided at P0 or at P1. You will always receive cells that are closer to naturally occurring chondrocytes than any other cell available.<\/p>\n Q: How do CELLvo™ Chondrocytes proliferate faster?<\/strong><\/p>\n When isolated and expanded using CELLvo™ Matrix, the cells proliferate and expand more naturally. In the chart below, data show a marked difference in P0 and P1 cells cultured on\u00a0CELLvo™ Matrix and tissue cultured polystyrene:<\/p>\n<\/div>\n<\/div>\n Cell expansion on different substrates for a set length of time. (A) P0 cells were cultured on CELLvo™ Matrix and tissue culture polystyrene for 8 days, and cell numbers were counted. (B) P1 cells were cultured on CELLvo™ Matrix and tissue culture polystyrene for 6 days, and cell numbers were counted. Data are presented as mean \u00b1 SD (n \u0003 3, ***p < 0.005)<\/p>\n<\/div>\n<\/figcaption><\/figure>\n<\/div>\n<\/div>\n<\/div>\n Q: How do CELLvo™ Human Chondrocytes impact my research study?<\/strong><\/p>\n In two ways: By maintaining natural phenotype as shown by the comparison of COL2A1\/COL1A1 ratio in the chart below, and, in the case of CELLvo™\u00a0Osteoarthritic and Healthy Chondrocytes, improved experimental design for cartilage therapies and regenerative medicine.<\/p>\n<\/div>\n<\/div>\n Dedifferentiation is characterized by an increased expression of fibroblast marker (type I collagen) and a decreased expression of chondrocyte markers (type II collagen and aggrecan)Relative gene expression in HAC cultured on CELLvo™ Matrix and tissue culture polystyrene for a similar PDL. The ratios of COL2A1\/COL1A1 of P0 cells were calculated as described in the Materials and methods section. Data are presented as mean \u00b1 SD (n = 4, *p < 0.05, **p < 0.01 and ***p < 0.005).<\/p>\n<\/div>\n<\/figcaption><\/figure>\n<\/div>\n<\/div>\n<\/div>\n Q: Why culture my Chondrocytes on CELLvo™\u00a0Matrix\u00a0versus other comparable products?<\/strong><\/p>\n Because chondrocytes cultured on the CELLvo™ Matrix are more responsive to stimuli, and are not \u201cbusy\u201d responding to a foreign microenvironment.\u00a0CELLvo™ Matrix is a naturally produced biomimetic substrate that allows chondrocytes to respond to experimental variables rather than an artificial substrate.<\/p>\n Q: Are CELLvo™\u00a0Human Chondrocytes and CELLvo™ Matrix\u00a0produced under GMP guidelines?<\/strong><\/p>\n The StemBioSys facility was built with GMP certification in mind. We have two different areas; One area is for making reagents used in the CELLvo™ Matrix process and preparing healthy donor cells for the process, and the second area is a Class 10,000 (ISO 7) room with HEPA filtered air and monitored positive pressure. The CELLvo™ Matrix process is performed in Class 100 (ISO 5) biological safety cabinets within the clean room. While our current product is for research use only and does not require GMP, we are manufacturing the product under the spirit of GMP requirements in anticipation that we will switch to full GMP certification in the near future.<\/p>\n<\/div>\n<\/div>\n CELLvo™ Human Chondrocytes are the only cells isolated and expanded using StemBioSys\u2019 CELLvo™ Matrix technology. Our process allows us to preserve more natural phenotypes. All of our cells are early-passage and xeno-free whenever possible.<\/p>\n <\/p>\n Introducing CELLvo™ Human Chondrocytes and CELLvo™ Matrix, A New Cell Culture System Young, healthy cells at early passage, or older, uncultured osteoarthritic cells \u2013 your choice. We offer the means for a deeper understanding of authentic chondrocyte behaviour. Broaden your<\/p>\n","protected":false},"author":13,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18],"tags":[540],"class_list":["post-5956","post","type-post","status-publish","format-standard","hentry","category-cell-culture","tag-stembiosys"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/5956","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=5956"}],"version-history":[{"count":9,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/5956\/revisions"}],"predecessor-version":[{"id":5965,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/5956\/revisions\/5965"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=5956"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=5956"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=5956"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}A Growth Substrate For Rapid Chondrocyte Expansion<\/h4>\n
\n<\/div>\n<\/div>\nFrequently Asked Questions:<\/h3>\n
![\"Cell](\"https:\/\/images.squarespace-cdn.com\/content\/v1\/5b9a76c64cde7aa1fc59e2be\/1590252891063-I69Z25J7M8XZ34XGGTUM\/ke17ZwdGBToddI8pDm48kGVAqUhYrGdhEGudzFls7K1Zw-zPPgdn4jUwVcJE1ZvWQUxwkmyExglNqGp0IvTJZamWLI2zvYWH8K3-s_4yszcp2ryTI0HqTOaaUohrI8PILoipRwDGDp8JlOmHWwEzCs15SnFJfrg4f5DR1NSbatA\/Artboard+1.png\")
<\/div>![\"Dedifferentiation](\"https:\/\/images.squarespace-cdn.com\/content\/v1\/5b9a76c64cde7aa1fc59e2be\/1590253137190-2DDVWK1BXTBR0M6PAFT5\/ke17ZwdGBToddI8pDm48kCj0mOQHRM2E-teWRpUG82JZw-zPPgdn4jUwVcJE1ZvWEtT5uBSRWt4vQZAgTJucoTqqXjS3CfNDSuuf31e0tVGXOdTr06PT-oJtgW1EBOzMT3oiaA7YOp3HRLbVwunpGN1lH3P2bFZvTItROhWrBJ0\/Artboard+2.png\")
<\/div>Learn More\u2026<\/h3>\n
View Products<\/a><\/h2>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"