{"id":8964,"date":"2023-10-26T16:26:32","date_gmt":"2023-10-26T16:26:32","guid":{"rendered":"https:\/\/www.caltagmedsystems.co.uk\/information\/?p=8964"},"modified":"2023-10-27T11:24:35","modified_gmt":"2023-10-27T11:24:35","slug":"in-vivo-gold-vs-in-vivo-platinum-antibodies","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/in-vivo-gold-vs-in-vivo-platinum-antibodies\/","title":{"rendered":"In vivo\u00a0GOLD™\u00a0vs. In vivo\u00a0PLATINUM™ Antibodies"},"content":{"rendered":"\n

In vivo<\/em>\u00a0grade antibodies have a variety of uses in biological research, from the discovery of cell signalling pathways to the manipulation of biological systems in animal models for pre-clinical studies.\u00a0In vivo<\/em>\u00a0antibodies from Leinco Technologies have the highest purity standards in the industry, with low endotoxin levels, no detectable aggregates nor leachable protein A. These antibodies are produced in their cGMP and ISO 9001:2015 \/ ISO 13485:2015 certified facility and are available in custom concentrations and package sizes.\u00a0In vivo\u00a0PLATINUM™ antibodies are also suitable for animal injection as they are pathogen-free as determined by the\u00a0IDEXX Impact I PCR mouse pathogen profile (see table 2 below).<\/p>\n\n\n\n

GOLD™ vs. PLATINUM™ in vivo<\/em> Antibody Quality Standards:<\/h3>\n\n\n\n
Specifications<\/strong><\/th>In vivo<\/em> GOLD™ MAbs<\/th>In vivo<\/em> PLATINUM™ MAbs<\/th><\/tr><\/thead>
Binding validation determined by
WB, FC or ELISA<\/strong><\/td>		Yes<\/td>Yes<\/td><\/tr>
Endotoxin level determined by
the LAL method<\/strong><\/td>		\u2264 1.0 EU\/mg<\/td>\u2264 0.5 EU\/mg<\/td><\/tr>
Antibody aggregation screening by analytical SEC<\/strong><\/td>\u2265 95% monomer<\/td>\u2265 98% monomer<\/td><\/tr>
Purity by SDS PAGE<\/strong><\/td>\u2265 95%<\/td>\u2265 98%<\/td><\/tr>
Formulation for in vivo<\/em> use<\/strong><\/td>No preservatives
No stabilisers
No carrier protein
Sterile PBS pH 7.2 \u2013 No K+<\/sup> or Ca2+<\/sup>
Concentration: > 5mg\/mL<\/td>		Functional grade preclinical antibodies are manufactured in an animal-free facility using only in vitro<\/em> protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.<\/td><\/tr>
Murine pathogen screening<\/strong><\/td>Not applicable, see PLATINUM functional grade antibodies<\/td>Pathogen tested (IMPACT1) (see table)<\/td><\/tr>
Applications<\/strong><\/td>In vivo<\/em> functional studies and may be used for WB, FC, IF or IHC<\/td><\/tr>
Product Preparation<\/strong><\/td>Functional grade preclinical antibodies are manufactured in an animal-free facility using only in vitro<\/em> protein-free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.<\/td><\/tr>
Storage and Handling<\/strong><\/td>Use a manual defrost freezer and avoid repeated freeze-thaw cycles.<\/strong>
\u2022 1 month, 2 to 8 \u00b0C under sterile conditions, as supplied.
\u2022 12 months, -70 \u00b0C under sterile conditions.<\/td><\/tr><\/tbody><\/table>
Table 1<\/figcaption><\/figure>\n\n\n\n

IDEXX IMPACT I (PCR-based) Mouse Pathogen Profile for Purified In vivo <\/em>PLATINUM™ Grade Antibodies:<\/h3>\n\n\n\n
Mycoplasma sp.<\/em><\/td>Mouse adenovirus 1 (MAV1)<\/td>Lactate dehydrogenase-elevating virus (LDEV)<\/td><\/tr>
Mycoplasma pulmonis<\/em><\/td>Mouse adenovirus 2 (MAV2)<\/td>Mouse kidney parvovirus (MKPV)<\/td><\/tr>
Sendai virus<\/td>Murine norovirus (MNV)<\/td>Hantaan Virus<\/td><\/tr>
Mouse hepatitis virus (MHV)<\/td>Reovirus 3 (REO3)<\/td>Mouse cytomegalovirus (mCMV)<\/td><\/tr>
Pneumonia virus of mice (PVM)<\/td>Mouse rotavirus (EDIM)<\/td>K virus<\/td><\/tr>
Minute virus of mice (MVM)<\/td>Ectromelia virus<\/td>Mouse thymic virus (MTV)<\/td><\/tr>
Mouse parvovirus (MPV)<\/td>Polyomavirus<\/td>Corynebacterium bovis<\/em><\/td><\/tr>
Theiler\u2019s murine encephalomyelitis (TMEV)<\/td>Lymphocytic choriomeningitis virus (LCMV)<\/td>Corynebacterium sp.<\/em><\/td><\/tr><\/tbody><\/table>
Table 2<\/figcaption><\/figure>\n\n\n\n
\n\n\n\n

Information Provided by Leinco Technologies Inc.<\/p>\n\n\n\n

Caltag Medsystems <\/a>is the distributor of Leinco Technologies’ products in the UK and Ireland. If you have any questions about these products, please contact us<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"

Leinco Technologies provides in vivo functional grade antibodies with the highest purity standards in the industry: In vivo GOLDTM & In vivo PLATINUMTM.<\/p>\n","protected":false},"author":13,"featured_media":8987,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[132,1],"tags":[185,735,566,734,736],"class_list":["post-8964","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-antibodies","category-general-information","tag-antibodies","tag-gold","tag-in-vivo","tag-leinco-technologies","tag-platinum"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/8964","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=8964"}],"version-history":[{"count":20,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/8964\/revisions"}],"predecessor-version":[{"id":9028,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/8964\/revisions\/9028"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media\/8987"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=8964"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=8964"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=8964"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}