{"id":9006,"date":"2023-10-27T10:35:12","date_gmt":"2023-10-27T10:35:12","guid":{"rendered":"https:\/\/www.caltagmedsystems.co.uk\/information\/?p=9006"},"modified":"2023-10-27T10:35:13","modified_gmt":"2023-10-27T10:35:13","slug":"phenocycler-fusion-codex-technology-and-workflow-steps","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/phenocycler-fusion-codex-technology-and-workflow-steps\/","title":{"rendered":"PhenoCycler-Fusion (CODEX)\u00ae\u00a0 Technology and Workflow Steps"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">PhenoCycler-Fusion (CODEX)\u00ae<strong>\u00a0<\/strong>Introduction<\/h2>\n\n\n\n<p>Overcoming challenges of traditional multiplexed immunofluorescence staining has now become easier with the PhenoCycler-Fusion (CODEX)\u00ae ultra-high multiplexed imaging system and assay reagents. This innovative, highly scalable technology targets subsets of an antibody panel through repetitive imaging cycles while revealing over 40 biomarkers in a single tissue section. The PhenoCycler-Fusion (CODEX)\u00ae technology was pioneered in Dr. Nolan\u2019s lab at Stanford and is now patented by\u00a0<a href=\"https:\/\/www.akoyabio.com\/\">Akoya Biosciences<\/a>.\u00a0<\/p>\n\n\n\n<h5 class=\"wp-block-heading\">The Benefits\u00a0<\/h5>\n\n\n\n<ul class=\"wp-block-list\" style=\"font-size:16px\">\n<li>A comprehensive end-to-end solution containing the fluidics platform, assay reagents, and bioinformatics<\/li>\n\n\n\n<li>Benchtop footprint that integrates with an existing fluorescence microscope<\/li>\n\n\n\n<li>The system is scalable and capable of imaging &gt;50 biomarkers per run<\/li>\n\n\n\n<li>Preserves the sample for Region Of Interest (ROI) analysis and H&amp;E staining<\/li>\n\n\n\n<li>FF and FFPE tissue-compatible<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">The Technology<\/h2>\n\n\n\n<h5 class=\"wp-block-heading\">Three main components of PhenoCycler-Fusion (CODEX)\u00ae\u00a0<\/h5>\n\n\n\n<p>Spatial biology multiplex imaging makes it possible to visualise in 3D and phenotype every cell across an entire microscope slide. By using DNA barcode technology comprised of unique\u00a0oligonucleotide sequences conjugated to an antibody. PhenoCycler-Fusion can help to scale up your spatial discovery efforts and translate those discoveries into actionable spatial phenotypic signatures.<\/p>\n\n\n\n<figure class=\"wp-block-image is-resized\"><a href=\"https:\/\/www.leinco.com\/wp-content\/uploads\/2022\/02\/Codex-Illustration.jpg\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.leinco.com\/wp-content\/uploads\/2022\/02\/Codex-Illustration.jpg\" alt=\"\" class=\"wp-image-91847\" style=\"width:456px;height:172px\" width=\"456\" height=\"172\"\/><\/a><\/figure>\n\n\n\n<h5 class=\"wp-block-heading clear\">Single-step staining to preserve sample integrity<\/h5>\n\n\n\n<p>The PhenoCycler-Fusion (CODEX)\u00ae fluidics instrument has an automated imaging cycle.\u00a0The first step is the staining of tissue\u00a0with >50 uniquely barcoded antibodies,\u00a0combined for a single tissue staining reaction.<\/p>\n\n\n\n<figure class=\"wp-block-image is-resized\"><a href=\"https:\/\/www.leinco.com\/wp-content\/uploads\/2022\/02\/Codex-Illustration-1.jpg\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.leinco.com\/wp-content\/uploads\/2022\/02\/Codex-Illustration-1.jpg\" alt=\"CODEX Technology - Tissue staining with antibody panel\" class=\"wp-image-91849\" style=\"width:457px;height:163px\" width=\"457\" height=\"163\"\/><\/a><\/figure>\n\n\n\n<h5 class=\"wp-block-heading clear\">An automated process of imaging biomarkers using the PhenoCycler-Fusion (CODEX)\u00ae\u00a0instrument and a companion microscope<\/h5>\n\n\n\n<p>Then, applied to the tissue and imaged are three reporter dyes with complimentary barcodes. Finally, the three reporter dyes are removed and the process is repeated until all antibodies have been imaged. Analyse 5 to 100+ slides per week.<\/p>\n\n\n\n<figure class=\"wp-block-image is-resized\"><a href=\"https:\/\/www.leinco.com\/wp-content\/uploads\/2021\/02\/Codex-Illustration-3-final-03.png\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.leinco.com\/wp-content\/uploads\/2021\/02\/Codex-Illustration-3-final-03.png\" alt=\"CODEX workflow steps\" class=\"wp-image-78970\" style=\"width:453px;height:356px\" width=\"453\" height=\"356\"\/><\/a><\/figure>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-alpha-channel-opacity has-background clear\" style=\"background-color:#21318f;color:#21318f\"\/>\n\n\n\n<p>Originally posted by Leinco Technologies Inc. on:\u00a0<a href=\"https:\/\/www.leinco.com\/codex_technology\/\">https:\/\/www.leinco.com\/codex_technology\/<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/www.caltagmedsystems.co.uk\/\">Caltag Medsystems&nbsp;<\/a>is the distributor of Leinco Technologies\u2019 products in the UK and Ireland. If you have any questions about these products, please&nbsp;<a href=\"https:\/\/www.caltagmedsystems.co.uk\/contact.php\">contact us<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>PhenoCycler-Fusion (CODEX)\u00ae technology target subsets of an antibody panel via repetitive imaging cycles, revealing 40+ biomarkers in a tissue section.<\/p>\n","protected":false},"author":13,"featured_media":8987,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[132,1,740],"tags":[734,742,741],"class_list":["post-9006","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-antibodies","category-general-information","category-spatial-biology","tag-leinco-technologies","tag-phenocycler","tag-spatial-biology"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/9006","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=9006"}],"version-history":[{"count":8,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/9006\/revisions"}],"predecessor-version":[{"id":9014,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/9006\/revisions\/9014"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media\/8987"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=9006"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=9006"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=9006"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}