{"id":9031,"date":"2023-10-29T17:09:46","date_gmt":"2023-10-29T17:09:46","guid":{"rendered":"https:\/\/www.caltagmedsystems.co.uk\/information\/?p=9031"},"modified":"2023-10-29T17:12:09","modified_gmt":"2023-10-29T17:12:09","slug":"2d-differentiation-of-epithelial-cells-fibroblasts-and-melanocytes-with-ease-and-accuracy","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/2d-differentiation-of-epithelial-cells-fibroblasts-and-melanocytes-with-ease-and-accuracy\/","title":{"rendered":"2D Differentiation of Epithelial Cells, Fibroblasts, and Melanocytes With Ease and Accuracy"},"content":{"rendered":"\n

Conventional 2D platforms are attractive to cell biologists due to their simplicity and efficiency. 2D monolayer cell culture enables steady nutrients and growth factors supply which results in homogenous growth and proliferation.<\/p>\n\n\n\n

The process of 2D differentiation is a highly orchestrated series of complex events that are significantly affected by the cell\u2019s environment. A wide range of factors in the proliferation media actively counteract the cell differentiation process. Therefore, it is recommended to use media that were specifically developed for differentiation, such as CELLnTEC\u2019s 2D differentiation media. These media come\u00a0in a fully supplemented, one-bottle ready-to-use formulation designed to create an environment conducive to cell differentiation. To culture cells prior to differentiation, we highly recommend using our respective proliferation media to ensure optimal performance.\u00a0<\/p>\n\n\n\n

Tissue Type<\/th>Proliferation Medium<\/th>2D Differentiation Medium<\/th><\/tr>
Keratinocytes<\/td>Epithelial Proliferation\u00a0CnT-PR<\/a><\/td>Epithelial Differentiation\u00a0CnT-PR-D<\/a><\/td><\/tr>
Fibroblasts<\/td>Fibroblast Proliferation\u00a0CnT-PR-F<\/a><\/td>Fibroblast ECM\u00a0CnT-PR-ECM<\/a><\/td><\/tr>
Melanocytes<\/td>Melanocyte Proliferation\u00a0CnT-40<\/a><\/td>Melanocyte Differentiation\u00a0CnT-PR-MD<\/a><\/td><\/tr>
Airway Epithelial<\/td>Epithelial Proliferation\u00a0CnT-PR-A<\/a><\/td>Melanocyte Differentiation\u00a0CnT-PR-AD<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

EPITHELIAL CELLS DIFFERENTIATION<\/strong><\/h3>\n\n\n\n

CELLnTEC’s Prime Epithelial 2D Differentiation (CnT-PR-D<\/a>) medium delivers complete differentiation of primary epidermal keratinocytes and most other epithelial cells, while large airway epithelial cells can be differentiated using our\u00a0CnT-Prime Airway Epithelial 2D\/3D Differentiation Medium\u00a0(CnT-PR-AD<\/a>). \u00a0<\/p>\n\n\n\n