{"id":9165,"date":"2024-09-03T16:24:41","date_gmt":"2024-09-03T16:24:41","guid":{"rendered":"https:\/\/www.caltagmedsystems.co.uk\/information\/?p=9165"},"modified":"2025-06-13T11:31:26","modified_gmt":"2025-06-13T11:31:26","slug":"tcr-tetramers","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/tcr-tetramers\/","title":{"rendered":"TCR Tetramers"},"content":{"rendered":"\n
\"\"<\/a><\/figure>\n\n\n\n

A TCR Tetramer is a reagent prepared by tetramerising biotinylated antigen-specific TCRs with the help of phycobiliprotein-labelled streptavidin.<\/p>\n\n\n\n

MBL has efficiently manufactured a TCR Tetramer reagent, which is recognised by MHC, and has successfully commercialised it. Moreover, our cancer antigen-specific (survivin-2B, PBF) TCR Tetramer reagents* have also been assessed for their staining properties by flow cytometry.<\/p>\n\n\n\n

*These products are licensed under JP patent No. P2017-169566A.<\/p>\n\n\n\n

\n
See all TCR Tetramers<\/strong><\/a><\/div>\n<\/div>\n\n\n\n

Preparation of TCR Tetramers<\/h3>\n\n\n\n

The manufacturing process is outlined in the figure below. Purified recombinant TCR \u03b1 and \u03b2 chains are refolded for several days to obtain soluble monomeric TCRs (monomers). The refolded monomer is conjugated with a single biotin molecule as the expressed recombinant \u03b2 chain is already biotinylated at the C-terminal end. The biotinylated monomers were purified and subsequently linked by adding fluorochrome-labeled streptavidin.<\/p>\n\n\n\n

\"\"<\/a><\/figure>\n\n\n\n

Comparison between MHC and TCR Tetramers<\/h3>\n\n\n\n
 <\/td>MHC TETRAMER<\/strong><\/td>TCR TETRAMER<\/strong><\/td><\/tr>
Structure<\/td>MHC\/peptide<\/td>TCR (\u03b1 chain\/\u03b2 chain)<\/td><\/tr>
Recognition<\/td>Specific TCR<\/td>Specific MHC\/peptide complex<\/td><\/tr>
Detection<\/td>Antigen-specific T cells<\/td> Antigen-specific tumours and antigen-presenting cells<\/td><\/tr>
Application<\/td>\u30fbQuantification
\u30fbMonitoring of antigen-specific T cells<\/td>		\u30fbQuantification
\u30fbEvaluation of the efficacy of cancer immunotherapies
\u30fbQuality control of cancer vaccine<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Staining using TCR Tetramers<\/h3>\n\n\n\n

Example #1: Cell Staining (Survivin-2B TCR Tetramer)<\/h4>\n\n\n\n

C1R cells (HLA-A-deficient) that stably expressed HLA-A*24:02 (called C1R-A24), were prepared; these cells were pulsed with survivin-2B peptide (AYACNTSTL) for 4 hours. After, the cells were stained with PE-labeled survivin-2B TCR Tetramer and Anti-HLA-A24 (Human) mAb-FITC (Code No. K0209-4) and further analysed by flow cytometry. 96.42% of HLA-A24+\/Tetramer+ cells were detected while staining peptide-pulsed C1R-A24 cells.<\/p>\n\n\n\n

\"\"
Numbers in the upper right quadrants represent the percentages of TCR Tetramer+ cells relative to the total HLA-A24+ cells<\/figcaption><\/figure>\n\n\n\n

Example #2: Staining of MHC Tetramer-conjugated beads (Survivin-2B TCR Tetramer)<\/h4>\n\n\n\n

Streptavidin-coated beads were loaded with biotinylated peptide\/HLA complex (HLA-A*24:02 presenting survivin-2B peptide, AYACNTSTL) at dilution ratios ranging from 1 to 10\u22123. This mimics the difference in the pMHC expression in cells.<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

The following data reveal the staining of the beads using Survivin-2B TCR Tetramer. A representative histogram indicates TCR Tetramer-positive populations in the normalized bead count. The noncoated beads were used as a negative control (R=0).<\/p>\n\n\n\n

The reactivity of TCR Tetramers depends on the number of peptide\/HLA complexes coated on the beads.<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n
\n\n\n\n

Information originally posted on: https:\/\/www.mblbio.com\/bio\/g\/product\/allergy-Immunology\/pickup\/tcrtetramer.html<\/a><\/p>\n\n\n\n

Caltag Medsystems <\/a>is the distributor of MBL <\/a>products in the UK and Ireland. If you have any questions about these products, please contact us<\/a>.<\/p>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

A TCR Tetramer is a reagent prepared by tetramerising biotinylated antigen-specific TCRs with the help of phycobiliprotein-labelled streptavidin.<\/p>\n","protected":false},"author":13,"featured_media":9171,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1,15],"tags":[67,933,224],"class_list":["post-9165","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-general-information","category-tetramer","tag-mbl-international","tag-tcr","tag-tetramer"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/9165","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=9165"}],"version-history":[{"count":6,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/9165\/revisions"}],"predecessor-version":[{"id":12326,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/9165\/revisions\/12326"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media\/9171"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=9165"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=9165"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=9165"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}