{"id":9528,"date":"2024-03-07T11:53:59","date_gmt":"2024-03-07T11:53:59","guid":{"rendered":"https:\/\/www.caltagmedsystems.co.uk\/information\/?p=9528"},"modified":"2025-05-16T15:38:53","modified_gmt":"2025-05-16T15:38:53","slug":"ha-tags-better-understanding-of-localisation-expression-and-purification-of-target-proteins","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/ha-tags-better-understanding-of-localisation-expression-and-purification-of-target-proteins\/","title":{"rendered":"HA-tags: Better Understanding of Localisation, Expression and Purification of Target Proteins"},"content":{"rendered":"\n

The Hemaglutin (HA) Epitope tag is a valuable method for fast detection and purification of expressing target proteins. Furthermore, HA epitope tagging enables the localisation and quantification of expressed proteins in a wide range of organisms. Most notably HA tagging is used for the study of poorly characterised proteins by way of recombinant DNA techniques. The tag is transfected into the cells of interest and expressed by the cell permitting the detection and\/or purification using a monoclonal antibody specific to the HA tag. The HA-tagged proteins are detected and purified using the same type of techniques that are used with standard protein-speci\ufb01c antibodies.<\/p>\n\n\n\n

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HA-Tags for Purification and Localisation<\/h2>\n\n\n\n
Product Code<\/th>Product Name<\/th>Target<\/th>Size<\/th><\/tr><\/thead>
MBL-561-5<\/a><\/td>Anti-HA-tag pAb<\/td>HA<\/td>500 \u00b5L<\/td><\/tr>
MBL-561-8<\/a><\/td>Anti-HA-tag pAb-Agarose (Polyclonal Antibody)<\/td>HA<\/td>Gel: 200 \u00b5L<\/td><\/tr>
MBL-M132-10<\/a><\/td>Anti-HA-tag mAb-Magnetic Agarose (Monoclonal Antibody)<\/td>HA<\/td>20 Tests (Gel: 200 \u03bcl)<\/td><\/tr>
MBL-M132-11<\/a><\/td>Anti-HA-tag mAb-Magnetic Beads (Monoclonal Antibody)<\/td>HA-tag<\/td>20 Tests (Slurry: 1 mL)<\/td><\/tr>
MBL-M132-3<\/a><\/td>Anti-HA-tag mAb (Monoclonal Antibody)<\/td>HA<\/td>100 \u00b5l<\/td><\/tr>
MBL-M180-10<\/a><\/td>Anti-HA-tag mAb-Magnetic Agarose (Monoclonal Antibody)<\/td>HA<\/td>20 Tests (Gel: 200 \u03bcl)<\/td><\/tr>
MBL-M180-11<\/a><\/td>Anti-HA-tag mAb-Magnetic Beads (Monoclonal Antibody)<\/td>HA-tag<\/td>20 Tests (Slurry: 1 mL)<\/td><\/tr>
MBL-M180-3<\/a><\/td>Anti-HA-tag mAb (Monoclonal Antibody)<\/td>HA<\/td>200 \u03bcL<\/td><\/tr>
MBL-M180-6<\/a><\/td>Anti-HA-tag mAb-Biotin (Monoclonal Antibody)<\/td>HA<\/td>50 \u00b5l<\/td><\/tr>
MBL-M180-7<\/a><\/td>Anti-HA-tag mAb-HRP-DirecT (Monoclonal Antibody)<\/td>HA<\/td>100 \u00b5l<\/td><\/tr>
MBL-M180-A48<\/a><\/td>Anti-HA-tag mAb-Alexa Fluor\u00ae 488 (Monoclonal Antibody)<\/td>HA<\/td>100 \u03bcg\/100 \u03bcL<\/td><\/tr>
MBL-M180-A59<\/a><\/td>Anti-HA-tag mAb-Alexa Fluor\u00ae 594 (Monoclonal Antibody)<\/td>HA<\/td>100 \u00b5l<\/td><\/tr>
MBL-M180-A64<\/a><\/td>Anti-HA-tag mAb-Alexa Fluor\u00ae 647 (Monoclonal Antibody)<\/td>HA<\/td>100 \u00b5l<\/td><\/tr>
MBL-3320<\/a><\/td>HA tagged Protein PURIFICATION KIT<\/td>HA<\/td>20 Tests<\/td><\/tr>
MBL-3320-205<\/a><\/td>HA-tag peptide<\/td>HA<\/td>2 mg x 5<\/td><\/tr>
MBL-3321<\/a><\/td>HA-tagged Protein Purification Gel<\/td>HA<\/td>1 mL<\/td><\/tr>
MBL-3342<\/a><\/td>HA-tagged Protein Magnetic Purification Kit<\/td>His<\/td>1 kit<\/td><\/tr>
MBL-3342A<\/a><\/td>HA-tagged Protein Magnetic Purification Kit (Trial Kit)<\/td>HA<\/td>1 kit<\/td><\/tr>
MBL-561MS<\/a><\/td>Anti-HA-tag pAb for My select sampler set<\/td>HA<\/td>20 \u03bcL<\/td><\/tr>
MBL-M180-3MS<\/a><\/td>Anti-HA-tag mAb for My select sampler set<\/td>HA<\/td>20 \u03bcg\/20 \u03bcL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

MBLI offers a comprehensive line of high-quality antibodies and kits for your epitope tag experiments. These products provide high sensitivity for purification experiments and many other applications, including common epitope tags (DDDDK, HA, Myc, His, V5, etc.) and antibodies to fusion proteins (GFP, RFP, etc.) with special tags (mini-AID-tag and others).<\/p>\n\n\n\n