{"id":9740,"date":"2024-04-11T15:27:19","date_gmt":"2024-04-11T15:27:19","guid":{"rendered":"https:\/\/www.caltagmedsystems.co.uk\/information\/?p=9740"},"modified":"2025-04-17T14:27:26","modified_gmt":"2025-04-17T14:27:26","slug":"netrin-1-neuron-guidance-factor-involved-in-ips-regulation-and-tumourigenesis","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/netrin-1-neuron-guidance-factor-involved-in-ips-regulation-and-tumourigenesis\/","title":{"rendered":"Netrin-1 \u2013 Neuron Guidance Factor Involved in iPS Regulation and Tumourigenesis"},"content":{"rendered":"\n

Netrin-1 is a member of the laminin-like protein family and was initially identified as a potent chemotactic molecule involved in axonal guidance and cell migration during embryonic development. It is a neuronal guidance protein (NGP) that triggers either attraction or repulsion effects on migrating axons of neurons, interacting with the receptors DCC or UNC5 (A-D).<\/p>\n\n\n\n

Netrin-1 serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis and is involved in tumourigenesis. Netrin-1 regulates inflammation and leukocyte infiltration, suggesting roles for netrin-1 in the immune response. Netrin-1 regulates endothelial cell adhesion and angiogenesis, macrophage migration and apoptosis, smooth muscle cells (SMCs) phenotypic dedifferentiation and mobility, chemokine activities, and inflammatory responses during atherosclerosis initiation and progression.<\/p>\n\n\n\n

Netrin-1 protects neurons from death during development and favours tumour epithelial cell survival in some types of cancers. It interacts with the orphan amyloid precursor protein (APP), a protein component of the amyloid plaques that are associated with Alzheimer’s disease (AD). Netrin-1 also inhibits the remyelination of neurons in Multiple Sclerosis (MS) (and other progressive demyelinating diseases) by inhibiting oligodendrocyte precursor migration. Netrin-1 has been described to be the 5th Element of classical iPS cell factors. Netrin-1’s function in protecting embryonic stem cells from apoptosis and the addition of recombinant Netrin-1 improves the generation of mouse and human iPS cells (induced Pluripotent Stem Cells).<\/p>\n\n\n\n

Members of the UNC5 receptor family (UNC5A-D) have been identified as dependence receptors whose functions depend on the availability of their ligand netrin-1. Through binding to netrin-1, these receptors transmit signals for cell survival, migration and differentiation, and participate in diverse physiological and pathological processes. In the lack of netrin-1, however, these receptors initiate apoptosis-inducing signals. Netrin-1 is therefore a prosurvival factor acting by blocking cell death induced by its unbound receptors.<\/p>\n\n\n\n

Netrin-1 and its receptors play a role in tumourigenesis and tumour progression. The expression of the UNC5 receptor family is down-regulated in a variety of human tumours and Netrin-1 is overexpressed in a large number of aggressive cancers. Expression aberrance of the UNC5 receptor family in tumours is caused by diverse mechanisms including genomic, epigenetic, transcriptional and post-transcriptional regulation. Blocking netrin-1 binding to its receptors induces apoptotic cell death in tumour cells. A recently developed novel therapy based on functional blocking antibodies aims at inhibiting the binding of Netrin-1 to these receptors to trigger cell death by apoptosis.<\/p>\n\n\n\n

\"\"<\/a>
Figure:<\/strong> Netrin-1 and the dependence receptor signalling pathways. Adapted from: Netrin-1 and its receptors in tumorigenesis: H. Arakawa; Nat. Rev. Cancer 4,<\/strong> 978 (2004)<\/figcaption><\/figure>\n\n\n\n

Literature References:<\/h4>\n\n\n\n
    \n
  1. Netrin-1 regulates somatic cell reprogramming and pluripotency maintenance: D. Ozmadenci, et al.; Nat. Commun. 6,<\/strong> 7398 (2015)<\/li>\n\n\n\n
  2. Revisiting Netrin-1: One Who Guides (Axons): N.P. Boyer & S.L. Gupton; Front. Cell Neurosci. 12,<\/strong> 221 (2018)<\/li>\n\n\n\n
  3. Netrin-1 in Glioblastoma Neovascularization: The New Partner in Crime? X. Vasquez, et al.; Int. J. Mol. Sci. 22,<\/strong> 8248 (2021)<\/li>\n\n\n\n
  4. UNC5 dependence receptor family in human cancer: A controllable double-edged sword: Y. Zhu, et al.; Cancer Lett. 516,<\/strong> 28 (2021)<\/li>\n\n\n\n
  5. Netrin-1: A Modulator of Macrophage Driven Acute and Chronic Inflammation: L. Ziegon & M. Schlegel; Int. J. Mol. Sci. 23,<\/strong> 275 (2021)<\/li>\n\n\n\n
  6. Netrin-1: An emerging player in inflammatory diseases: X. Xia, et al.; Cytokine Growth Factor Rev. 64,<\/strong> 46 (2022)<\/li>\n<\/ol>\n\n\n\n

    Potent Functional Antibody for Blocking Netrin-1 Signaling Pathways In Vivo<\/em><\/h2>\n\n\n\n

    anti-Netrin-1 (human), mAb (rec.) (blocking) (2F5) (preservative-free) <\/h3>\n\n\n\n

    AG-27B-0018PF<\/a> (100 \u00b5g, 500 \u00b5g sizes & Bulk from Stock)<\/p>\n\n\n\n

    AdipoGen Life Sciences is the developer and manufacturer of the recombinant antibody clone 2F5, a potent antibody that inhibits the activation of human and mouse Netrin-1 to human or mouse receptors DCC or UNC5 (KD antibody-Netrin-1 is 1.5nM) for in vivo<\/em> blocking application. This antibody has been used successfully in scientific publications on neurodegeneration, tumourigenesis, angiogenesis and arthritis research.<\/p>\n\n\n\n

    This antibody is available as a preservative-free version and in BULK quantities for long-term studies.<\/p>\n\n\n\n

    Product Specifications:<\/h4>\n\n\n\n

    Clone<\/strong>:                2F5
    Isotype<\/strong>:              Human IgG2\u03bb
    Immunogen<\/strong>:      Recombinant human Netrin-1
    Applications<\/strong>:     Functional (Blocking), ELISA
    Crossreactivity<\/strong>: Human, Mouse
    Purity<\/strong>:                <95% (SDS-PAGE), Protein-A affinity purified.<\/p>\n\n\n\n

    \"\"<\/a>
    Figure:<\/strong> anti-Netrin-1 (human), mAb (rec.) (blocking) (2F5) (preservative free) (Prod. No. AG-27B-0018PF<\/a>) blocks tumour growth in vivo<\/em>. Method:<\/strong> Tumour cells (OCI-Ly3) were implanted in SCID mice by subcutaneous injection of 3×106 cells in 100\u00b5l of PBS. When tumours reached 150mm3, mice received intraperitoneal injections of blocking anti-Netrin-1 mAb (2F5) at 20 mg\/kg or an equal volume of the antibody control anti-Netrin-1 (human), mAb (rec.) (H4) (preservative free) (Prod. No. AG-27B-0020PF<\/a>) every two days. Tumor growth rates from the beginning of treatment are shown.<\/figcaption><\/figure>\n\n\n\n

    Selected Product References:<\/h4>\n\n\n\n
      \n
    1. Epidermal Growth Factor Receptor-Dependent Mutual Amplification between Netrin-1 and the Hepatitis C Virus: M.L. Plissonnier, et al.; PLoS Biol. 14,<\/strong> e1002421 (2016)<\/li>\n\n\n\n
    2. Targeting netrin-1\/DCC interaction in diffuse large B-cell and mantle cell lymphomas: T. Broutier, et al.; EMBO Mol. Med. 8,<\/strong> 96 (2016)<\/li>\n\n\n\n
    3. Netrin-1 acts as a non-canonical angiogenic factor produced by human Wharton\u2019s jelly mesenchymal stem cells (WJ-MSC): C.P. Prieto, et al.; Stem Cell Res. Ther. 8,<\/strong> 43 (2017)<\/li>\n\n\n\n
    4. Downregulation of the Netrin-1 Receptor UNC5b Underlies Increased Placental Angiogenesis in Human Gestational Diabetes Mellitus: C.P. Prieto, et al.; Int. J. Mol. Sci. 20,<\/strong> 1408 (2019)<\/li>\n\n\n\n
    5. Netrin-1 and its receptor DCC modulate survival and death of dopamine neurons and Parkinson\u2019s disease features: M. Jasmin, et al.; EMBO J. 40,<\/strong> e105537 (2021)<\/li>\n\n\n\n
    6. Netrin-1 Secreted by Human Osteoarthritic Articular Chondrocytes Promotes Angiogenesis in Vitro: J. Akoum, et al.; Cartilage 13,<\/strong> 94 (2022)<\/li>\n<\/ol>\n\n\n\n

      Biologically Active Netrin-1 for iPS Cells Generation: The 5th Key Reprogramming Factor<\/h2>\n\n\n\n

      Netrin-1 (human): Fc (human) (rec.)<\/h3>\n\n\n\n

      AG-40B-0075<\/a> (10 \u00b5g, 3 x 10 \u00b5g; 100 \u00b5g sizes & Bulk from Stock)<\/p>\n\n\n\n

      Netrin-1 (human) (rec.)<\/h3>\n\n\n\n

      AG-40B-0040<\/a> (10 \u00b5g, 3 x 10 \u00b5g; 100 \u00b5g sizes & Bulk from Stock)<\/p>\n\n\n\n

      AdipoGen Life Sciences is the developer and manufacturer of biologically active human Netrin-1. These proteins have a unique and improved formulation which leads to higher protein stability & activity, no aggregation nor precipitation (as known for several preparations from competitors), and no inclusion of BSA. The proteins cross-react with the human, mouse and rat receptors and have been published in scientific literature.<\/p>\n\n\n\n

      These recombinant proteins are available in BULK quantities.<\/p>\n\n\n\n

      Selected Product References:<\/h4>\n\n\n\n
        \n
      1. DCC constrains tumour progression via its dependence receptor activity: M. Castets, et al.; Nature 482,<\/strong> 534 (2011)<\/li>\n\n\n\n
      2. Netrin-1 regulates somatic cell reprogramming and pluripotency maintenance: D. Ozmadenci, et al.; Nat. Commun. 6,<\/strong> ID7398 (2015)<\/li>\n\n\n\n
      3. Targeting netrin\u20101\/DCC interaction in diffuse large B\u2010cell and mantle cell lymphomas: L. Broutier, et al.; EMBO Mol. Med. 8,<\/strong> 96 (2016)<\/li>\n\n\n\n
      4. Structural Decoding of the Netrin-1\/UNC5 Interaction and its Therapeutical Implications in Cancers: M. Grandin, et al.; Cancer Cell 29,<\/strong> 173 (2016)<\/li>\n\n\n\n
      5. Ultrasound molecular imaging as a non-invasive companion diagnostic for netrin-1 interference therapy in breast cancer: J. Wischhusen, et al.; Theranostics 8,<\/strong> 5126 (2018)<\/li>\n<\/ol>\n\n\n\n

        All Netrin-Related Research Products  <\/h2>\n\n\n\n
        Product Name<\/th>Product Code<\/th>Isotype<\/th>Applications<\/th>Species<\/th><\/tr><\/thead>
        anti-Netrin-1 (human), mAb (rec.) (blocking) (2F5) (preservative-free)<\/td>AG-27B-0018PF<\/a><\/td>Human IgG2\u03bb<\/td>Functional, ELISA<\/td>Human, Mouse<\/td><\/tr>
        anti-Netrin-1 (human), mAb (rec.) (H4) (preservative-free)<\/td>AG-27B-0020PF<\/a><\/td>Human IgG2\u03bb<\/td>Functional (negative control), ELISA<\/td>Human, Mouse<\/td><\/tr>
        anti-Netrin-4, mAb (Nely-1)<\/td>AG-20B-0039<\/a><\/td>Mouse IgG1\u03ba<\/td>ELISA, Western Blot<\/td>Human, Mouse<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
        Product Name<\/th>Product Code<\/th>Source<\/th>Endotoxin<\/th>Species<\/th><\/tr><\/thead>
        Netrin-1 (human):Fc (human) (rec.)<\/td>AG-40B-0075<\/a><\/td>HEK 293 cells<\/td><0.1EU\/\u03bcg<\/td>Human, Mouse, Rat<\/td><\/tr>
        Netrin-1 (human) (rec.)<\/td>AG-40B-0040<\/a><\/td>HEK 293 cells<\/td><0.01EU\/\u03bcg<\/td>Human, Mouse, Rat<\/td><\/tr>
        UNC5B (human):Fc (human) (rec.)<\/td>AG-40B-0037<\/a><\/td>HEK 293 cells<\/td><0.1EU\/\u03bcg<\/td>Human, Mouse<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
        \n
        Read About Netrin-1 on Adipogen’s Website<\/strong><\/a><\/div>\n\n\n\n
        View Netrin-1 Flyer<\/strong><\/a><\/div>\n<\/div>\n\n\n\n
        <\/div>\n\n\n\n
        \n\n\n\n

        Originally posted by Adipogen on: https:\/\/adipogen.com\/netrin<\/a><\/p>\n\n\n\n

        Caltag Medsystems <\/a>is the distributor of Adipogen<\/a> products in the UK and Ireland. If you have any questions about these products, please contact<\/a> us<\/a>.<\/p>\n\n\n\n

        <\/p>\n","protected":false},"excerpt":{"rendered":"

        AdipoGen Life Sciences is the developer and manufacturer of the recombinant antibody clone 2F5 and biologically active human Netrin-1.<\/p>\n","protected":false},"author":13,"featured_media":9746,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[115,10,1,622],"tags":[279,835,337,836],"class_list":["post-9740","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-apoptosis","category-cancer","category-general-information","category-inflammation","tag-adipogen","tag-netrin-1","tag-neurons","tag-tumourigenesis"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/9740","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=9740"}],"version-history":[{"count":6,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/9740\/revisions"}],"predecessor-version":[{"id":12156,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/9740\/revisions\/12156"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media\/9746"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=9740"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=9740"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=9740"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}