{"id":9930,"date":"2024-04-18T12:15:41","date_gmt":"2024-04-18T12:15:41","guid":{"rendered":"https:\/\/www.caltagmedsystems.co.uk\/information\/?p=9930"},"modified":"2024-04-18T12:15:41","modified_gmt":"2024-04-18T12:15:41","slug":"transduction-reagents-virus-capture-concentration-and-conservation","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/transduction-reagents-virus-capture-concentration-and-conservation\/","title":{"rendered":"Transduction Reagents: Virus Capture, Concentration and Conservation"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">For Virus Capture &amp; Concentration<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">Mag4C Kits<\/h3>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><a href=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/image-211.png\"><img loading=\"lazy\" decoding=\"async\" width=\"582\" height=\"772\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/image-211.png\" alt=\"\" class=\"wp-image-9931\" style=\"width:338px;height:auto\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/image-211.png 582w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/image-211-226x300.png 226w\" sizes=\"auto, (max-width: 582px) 100vw, 582px\" \/><\/a><\/figure>\n\n\n\n<p>The Mag4C Kits are based on a magnetic virus concentration technology (magnetic nanoparticles).<\/p>\n\n\n\n<p>Mag4C&nbsp;magnetic nanoparticles capture viruses in culture media with 80-99 % efficiency. Once captured onto magnetic beads, viruses can be:<\/p>\n\n\n\n<ul class=\"wp-block-list\" style=\"font-size:16px\">\n<li>Concentrated and stored with the Storage Buffer or directly used for a downstream assay.<\/li>\n\n\n\n<li>Concentrated, eluted from the magnetic beads with the Elution Buffer and stored with the Storage Buffer or used for subsequent assays.<\/li>\n<\/ul>\n\n\n\n<p>The Conservation Buffer has been expressly designed to improve the stability of viruses upon storage conditions and is fully compatible with magnetic nanoparticles.<\/p>\n\n\n\n<p>A Multipurpose Magnetic Separation Rack for 50, 15 or 1.5 mL tubes is also proposed. It can hold 12 standard microtubes, two 15 mL and two 50 mL tubes.<\/p>\n\n\n\n<p class=\"clear\">Mag4C-LV\u00a0Kit presents unique properties:<\/p>\n\n\n\n<ul class=\"wp-block-list\" style=\"font-size:16px\">\n<li>Simple, rapid &amp; ready to use.<\/li>\n\n\n\n<li>Concentrates viruses by magnetic capture in 30-45 minutes.<\/li>\n\n\n\n<li>High yield of viral capture and recovery.<\/li>\n\n\n\n<li>No need to process magnetic beads before capture.<\/li>\n\n\n\n<li>Avoid ultracentrifugation, precipitation and chemicals &#8211; reduces handling steps of viruses.<\/li>\n\n\n\n<li>Suitable for all conditions &amp; viruses.<\/li>\n\n\n\n<li>Improve virus stability under storage conditions.<\/li>\n\n\n\n<li>Ideal for cell culture infection (Magnetofection\u2122&nbsp;advantages).<\/li>\n<\/ul>\n\n\n\n<p><strong><a href=\"https:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/index.php?group_1=All&amp;group_2=All&amp;supplier=76&amp;clonality=All&amp;host=All&amp;species=All&amp;applications=All&amp;regulatory_status=All&amp;searchwords=Mag4C%20LV\">Mag4C-LV<\/a><\/strong>\u00a0 is composed of 3 reagents allowing Magnetic Capture\/Concentration, Elution and Conservation of Lentiviruses and Retroviruses.<\/p>\n\n\n\n<p><strong><a href=\"https:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/index.php?group_1=All&amp;group_2=All&amp;supplier=76&amp;clonality=All&amp;host=All&amp;species=All&amp;applications=All&amp;regulatory_status=All&amp;searchwords=Mag4C%20AD\">Mag4C-Ad<\/a><\/strong> is composed of 3 reagents allowing Magnetic Capture\/Concentration, Elution and Conservation of Adenoviruses.<\/p>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-a89b3969 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link has-text-color has-background has-link-color wp-element-button\" href=\"https:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/index.php?group_1=All&amp;group_2=All&amp;supplier=76&amp;clonality=All&amp;host=All&amp;species=All&amp;applications=All&amp;regulatory_status=All&amp;searchwords=Mag4C\" style=\"color:#fbc100;background-color:#21318f\">See all Mag4C Kits<\/a><\/div>\n<\/div>\n\n\n\n<h3 class=\"wp-block-heading\">Viro-PEG<\/h3>\n\n\n\n<p><strong>Viro-PEG Lentivirus Concentrator<\/strong>\u00a0is a ready-to-use solution optimised for the capture and concentration of lentiviral particles, providing an easy and straightforward method to efficiently concentrate lentiviral particles without using ultracentrifugation.<\/p>\n\n\n\n<p>The virus can be concentrated over 100x with an efficiency ranging from 70 to 95%.<\/p>\n\n\n\n<p>Simply mix 1 volume of Lenticoncentrator with 4 volumes of lentiviral supernatant and spin the mixture in a standard centrifuge after the recommended incubation time.<br><\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Key benefits of Viro-PEG Lentivirus Concentrator:<\/h4>\n\n\n\n<ul class=\"wp-block-list\" style=\"font-size:16px\">\n<li>Increase lentiviral titers by up to 100x.<\/li>\n\n\n\n<li>Simple &amp; convenient &#8211; protocol adaptable from 4 hours to overnight incubation.<\/li>\n\n\n\n<li>Allow rescuing low-titer viral particles.<\/li>\n\n\n\n<li>No ultracentrifugation is required.<\/li>\n\n\n\n<li>Ready-to-use &amp; non-toxic to target cells.<\/li>\n<\/ul>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-a89b3969 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link has-text-color has-background has-link-color wp-element-button\" href=\"https:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/index.php?group_1=All&amp;group_2=All&amp;supplier=76&amp;clonality=All&amp;host=All&amp;species=All&amp;applications=All&amp;regulatory_status=All&amp;searchwords=viro-peg\" style=\"color:#fbc100;background-color:#21318f\">See all Viro-PEG<\/a><\/div>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\">For Virus Conservation<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">Mag4C Conservation Buffer<\/h3>\n\n\n\n<p><strong>Mag4C Conservation Buffer<\/strong>\u00a0can be added to viral stocks for long-term storage. It allows maintaining virus infectivity even after multiple freeze\/thawing cycles. Two different buffers are available depending on the virus: <strong>Ad-Conservation Buffer<\/strong> for adenoviruses &amp; <strong>Lv-Conservation Buffer<\/strong> for lentiviruses and retroviruses.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">The Conservation Buffer:<\/h4>\n\n\n\n<p>1. Preserves virus infectivity during storage.<\/p>\n\n\n\n<p>2. Minimises the loss of viral titers during storage and freeze\/thaw cycles.<\/p>\n\n\n\n<p>3. Can be used to conserve viruses bound to Mag4C beads.<\/p>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-a89b3969 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link has-text-color has-background has-link-color wp-element-button\" href=\"https:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/index.php?group_1=All&amp;group_2=All&amp;supplier=76&amp;clonality=All&amp;host=All&amp;species=All&amp;applications=All&amp;regulatory_status=All&amp;searchwords=conservation%20buffer\" style=\"color:#fbc100;background-color:#21318f\">See all Conservation Buffers<\/a><\/div>\n<\/div>\n\n\n\n<p class=\"clear\"> <\/p>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-alpha-channel-opacity has-background\" style=\"background-color:#21318f;color:#21318f\"\/>\n\n\n\n<p>Information provided by&nbsp;<a href=\"https:\/\/www.caltagmedsystems.co.uk\/ozbiosciences\/\">OZ Biosciences<\/a>.<\/p>\n\n\n\n<p><a href=\"https:\/\/www.caltagmedsystems.co.uk\/\">Caltag Medsystems&nbsp;<\/a>is the distributor of&nbsp;<a href=\"https:\/\/www.caltagmedsystems.co.uk\/ozbiosciences\/\">OZ Biosciences<\/a>&nbsp;products in the UK and Ireland. If you have any questions about these products, please&nbsp;<a href=\"https:\/\/www.caltagmedsystems.co.uk\/contact.php\">contact us<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>OZ Biosciences provides a selection of transduction reagents for the capture, concentration, and conservation of viruses.<\/p>\n","protected":false},"author":13,"featured_media":9807,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1,787],"tags":[542,868,870,871,872],"class_list":["post-9930","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-general-information","category-transduction","tag-oz-biosciences","tag-transduction-reagents","tag-virus-capture","tag-virus-concentration","tag-virus-conservation"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/9930","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=9930"}],"version-history":[{"count":7,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/9930\/revisions"}],"predecessor-version":[{"id":9938,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/9930\/revisions\/9938"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media\/9807"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=9930"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=9930"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=9930"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}