{"id":9939,"date":"2024-04-18T12:28:04","date_gmt":"2024-04-18T12:28:04","guid":{"rendered":"https:\/\/www.caltagmedsystems.co.uk\/information\/?p=9939"},"modified":"2024-04-18T12:28:05","modified_gmt":"2024-04-18T12:28:05","slug":"transduction-reagents-virus-production","status":"publish","type":"post","link":"https:\/\/www.caltagmedsystems.co.uk\/information\/transduction-reagents-virus-production\/","title":{"rendered":"Transduction Reagents: Virus Production"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">HYVIR\u2122<\/h2>\n\n\n\n<p><strong>HYVIR\u2122<\/strong> is a\u00a0novel lipid-based transfection kit\u00a0dedicated to\u00a0enhancing viral particle production in HEK-293T.<\/p>\n\n\n\n<p>Its specific formulation allows optimal plasmid compaction, protection and delivery rendering\u00a0HYVIR\u00a0ideal for the production of the last generation of lentivirus.<\/p>\n\n\n\n<p>3<sup>rd<\/sup>\u00a0generation system, while improving the safety of the 2<sup>nd<\/sup>\u00a0generation by splitting the packaging system into two plasmids, also increases the difficulty of delivery: now 4 plasmids need to be transfected at the same time under a precise stoichiometry.<\/p>\n\n\n\n<p>Thanks to its chemical moieties,\u00a0HYVIR\u2122\u00a0is the perfect transfection reagent dedicated to this task as it allows maximal compaction for an efficient co-transfection of packaging plasmid(s), envelope plasmid and lentiviral transfer plasmid encoding your gene of interest.<\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><a href=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/image-212.png\"><img loading=\"lazy\" decoding=\"async\" width=\"747\" height=\"535\" src=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/image-212.png\" alt=\"\" class=\"wp-image-9940\" style=\"width:340px;height:auto\" srcset=\"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/image-212.png 747w, https:\/\/www.caltagmedsystems.co.uk\/information\/wp-content\/uploads\/image-212-300x215.png 300w\" sizes=\"auto, (max-width: 747px) 100vw, 747px\" \/><\/a><\/figure>\n\n\n\n<ul class=\"wp-block-list\" style=\"font-size:16px\">\n<li><strong>Performant<\/strong><strong>:<\/strong>\u00a0reach high-titer functional lentiviral \/ virus production.<\/li>\n\n\n\n<li><strong>Highly efficient<\/strong>: co-transfection of 4 plasmids at the same time.<\/li>\n\n\n\n<li><strong>Reliable &amp; Reproducible<\/strong>: easily scalable for production in large volumes and can be raised to superior grade.<\/li>\n\n\n\n<li><strong>Ideal reagent for HEK293T cell\u00a0transfection<\/strong>.<\/li>\n\n\n\n<li><strong>Completely animal-origin-free<\/strong>.<\/li>\n<\/ul>\n\n\n\n<div class=\"wp-block-buttons clear is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-a89b3969 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link has-text-color has-background has-link-color wp-element-button\" href=\"https:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/index.php?group_1=All&amp;group_2=All&amp;supplier=76&amp;clonality=All&amp;host=All&amp;species=All&amp;applications=All&amp;regulatory_status=All&amp;searchwords=HYVIR\" style=\"color:#fbc100;background-color:#21318f\">See all HYVIR<sup>TM<\/sup><\/a><\/div>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\">CaPO<\/h2>\n\n\n\n<p><strong>Calcium Phosphate (CaPO) Transfection Kit<\/strong>\u00a0is perfect for transfecting HEK 293 cells. This transfection method, first described by Graham and Van Der Ebb in 1973, has been optimised in order to reach higher transfection efficiency. The CaPO transfection kit is simple and easy to use. It allows reaching between 95 and 100 % of HEK 293 transfected cells and a very high titer for virus production.\u00a0<\/p>\n\n\n\n<ul class=\"wp-block-list\" style=\"font-size:16px\">\n<li>Ideal for virus production.<\/li>\n\n\n\n<li>High HEK 293 cell transfection efficiency.<\/li>\n\n\n\n<li>Serum compatible.<\/li>\n\n\n\n<li>Simple, ready to use and Rapid.<\/li>\n<\/ul>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-a89b3969 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link has-text-color has-background has-link-color wp-element-button\" href=\"https:\/\/www.caltagmedsystems.co.uk\/pricing_ordering\/index.php?group_1=All&amp;group_2=All&amp;supplier=76&amp;clonality=All&amp;host=All&amp;species=All&amp;applications=All&amp;regulatory_status=All&amp;searchwords=CaPO\" style=\"color:#fbc100;background-color:#21318f\">See all CaPO<\/a><\/div>\n<\/div>\n\n\n\n<p class=\"clear\"> <\/p>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-alpha-channel-opacity has-background\" style=\"background-color:#21318f;color:#21318f\"\/>\n\n\n\n<p>Information provided by&nbsp;<a href=\"https:\/\/www.caltagmedsystems.co.uk\/ozbiosciences\/\">OZ Biosciences<\/a>.<\/p>\n\n\n\n<p><a href=\"https:\/\/www.caltagmedsystems.co.uk\/\">Caltag Medsystems&nbsp;<\/a>is the distributor of&nbsp;<a href=\"https:\/\/www.caltagmedsystems.co.uk\/ozbiosciences\/\">OZ Biosciences<\/a>&nbsp;products in the UK and Ireland. If you have any questions about these products, please&nbsp;<a href=\"https:\/\/www.caltagmedsystems.co.uk\/contact.php\">contact us<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>OZ Biosciences provides a novel lipid-based transfection kit dedicated to enhancing viral particle production.<\/p>\n","protected":false},"author":13,"featured_media":9928,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1,787],"tags":[542,868,873],"class_list":["post-9939","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-general-information","category-transduction","tag-oz-biosciences","tag-transduction-reagents","tag-virus-production"],"_links":{"self":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/9939","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/comments?post=9939"}],"version-history":[{"count":4,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/9939\/revisions"}],"predecessor-version":[{"id":9944,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/posts\/9939\/revisions\/9944"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media\/9928"}],"wp:attachment":[{"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/media?parent=9939"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/categories?post=9939"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.caltagmedsystems.co.uk\/information\/wp-json\/wp\/v2\/tags?post=9939"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}