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Exosomes - Feb 2018

The Problem with Ultracentrifugation for Exosome Isolation

Ultracentrifugation is regarded as the gold standard method for exosome isolation. The ultracentrifugation process is time consuming and may introduce protein and nucleic acid contamination. Pre-clearing samples by one or more low speed centrifugation steps will deplete the cells, platelets, and large apoptotic bodies. Larger extracellular vesicles can be pelleted at forces between 10000-20000 x g. Smaller extracellular vesicles can be pelleted at higher speeds, ranging from 100000-200000 x g. Depending on the protocol, it can take up to 24 hours to isolate exosomes by ultracentrifugation. It is impossible to achieve absolute separation due to potential contamination of proteins and nucleic acids...

ExoCap™ Streptavidin Kit - Faster, Easier and Customisable
The ExoCap™ Streptavidin Kit protocol is very easy and simple to follow. Researchers can isolate and enrich for exosomes of interest by coupling biotinylated molecules to the magnetic Streptavidin beads. There is no need for pre-treatment of samples because there is a direct incubation of sample with the Streptavidin magnetic beads. The magnetic Streptavidin beads coupled with biotinylated antibodies will selectively capture exosomes of interest. During the wash step the captured exosomes on the magnetic bead are retained, while any contaminants are removed, resulting in high purity exosomes that can be used for downstream analyses in as little as 4 hours.

Angiogenesis, Cell Culture, France - Feb 2018

Nouveau: Mesure de l’angiogénèse en temps réel en utilisant HUVEC GFP labellisé

Le GFP V2a Kit ™ - Vasculogénèse à l'Angiogénèse, est un modèle de co-culture de cellules primaires humaines validées, in vitro, de l'angiogenèse. Il permet la mesure dynamique de toutes les phases du processus angiogénique.

Small Molecules - Feb 2018

2017 Top 100 Small Molecules for your Apoptosis and Epigentics Research

Meet APExBIO –  An apoptosis and epigentics company. 

APExBIO provides a broad range of Apoptosis, Epigenetics, and Cancer Biology research reagents.  Plus custom services including peptide synthesis, antibody production and assay development.

1814 - Feb 2018

Newsletter

Welcome to the Caltag Medsystems Product Highlights! Make sure to try our quiz at the end of the newsletter!
  1. Blood Products Collected with Donor Specifications
  2. Not All Primary Cells are Created Equal
  3. ELISA Kits: Over 10,000 Available
  4. Inflammasome Signalling
  5. Measure Angiogenesis in Real Time Using GFP Labelled HUVECs
  6. Fresh & Cryopreserved Human and Animal Hepatocytes
  7. Toxins: Tetanus, Cholera and Other Highly Purified Bacterial Toxins
  8. Save Time and Money When Performing qPCR
  9. Immune Checkpoint Target: LIGHT Testing Set
  10. Brainteaser: Which Word?

Cytomark - Feb 2018

Prevent Cellular Degradation Within Cerebrospinal Fluid With TransFix

The invasion of leucocytes into cerebrospinal fluid (CSF) has grave prognostic significance in leukaemic patients. Flow cytometric analysis provides a highly specific diagnostic tool, however cells within CSF are typically low in concentration and degrade quickly. This results in the necessity to stabilise CSF specimens to achieve accurate analysis.

TransFix® is a stabilisation solution which has been shown to stabilise the cellular component of cerebrospinal fluid. TransFix has therefore been has been recommended for use in guidelines published in the British Journal of Haematology (case study 1) among others... 

Blood - Jan 2018

Providing fresh blood and bone marrow products from East Coast, USA

Almost one year ago, AllCells opened a new collection facility in Quincy, Massachusetts to better serve their European customers. Products available from Quincy are fresh haematologic tissue such as whole bone marrow, leukapheresis products, and whole blood sourced from qualified, clinically healthy human donors. Depending on your location, delivery within 24 hours of collection from the donor may be achievable.

Cytomark - Dec 2017

Reduce Variability in Clinical Trials by Stabilizing Samples

TransFix/EDTA Vacuum Blood Collection Tubes (TVTs) have been cleared for IVD use in the United States and Europe. Prefilled with TransFix and K3EDTA, these tubes allow for convenient collection, anticoagulation, and instant stabilization of peripheral blood for flow cytometric analysis.
  • Benefits for Clinical Research
  • Case Study: The Use of TransFix in Clinical Research
  • Inter-Laboratory Comparison of TVTs vs Competing Products
  • Find Out More

France, Cell Culture, Newsletter - Nov 2017

Solutions en sciences de la vie : Solutions d’angiogenèse, clonage d’hybridome, service d'approvisionnement pour les tissus, cellules animales, cellules primaires et milieux.

Bienvenue dans le bulletin de Caltag Medsystems. Vous trouverez une sélection de nouveaux produits, d’offres spéciales ainsi que les dernières nouveautés distribuées par Caltag Medsystems.

  • Solutions d'angiogenèse
  • Améliorer l'efficacité du clonage d’hybridome
  • Service d'approvisionnement pour les tissus
  • Cellules animales
  • Cellules primaires et milieux de haute qualité 

Small Molecules - Nov 2017

Small Molecules for your Apoptosis and Epigentics Research

APExBIO provides a broad range of Apoptosis, Epigenetics, and Cancer Biology research reagents.  Plus custom services including peptide synthesis, antibody production and assay development.

ELISpot - Nov 2017

Measure Immune Response & Treatment Efficacy with Diaclone ELISpot Kits

Monitoring the immune responses of patients treated with immunotherapy is essential to evaluate the efficacy of treatment and correlate clinical responses to Cytotoxic T Lymphocyte responses. Diaclone ELISPot assays are specific, accurate, sensitive, precise & robust. This bioassay is recognised as the reference method to evaluate vaccine efficacy in clinical trials and has an expanding role in many immune related research & clinical fields.

Find out More

View ELISpot Products

Download application notes: “Diaclone ELISpot: monitoring the T-Cell response to tumour antigens

Download the Diaclone ELISpot Brochure


Next Generation Sequencing - Nov 2017

Fast and Easy Targeted Library Preparation for Next Generation Sequencing

We offered a solution earlier this year to alleviate NGS workflow bottlenecks and improve the speed and accuracy of sequencing with Paragon Genomics.

To continue to improve and streamline your NGS targeted library preparations, Paragon Genomics is now offering two new products.

Platelets - Nov 2017

The Future of Platelet Function Testing

Stabilisation of Blood Samples for Reliable Analysis of Platelet Function.

Platelet Solutions' products stabilise samples prior to assessment of platelet function. This provides flexibility in how, when and where platelet measurements are made without the need for specialised equipment or technical expertise.

Reliable analysis of platelet activation markers, platelet aggregates or VASP phosphorylation can then be conducted at an alternative location or at a later time to suit your needs. All samples can be collected and processed using easy-to-use kits, by anyone with expertise in blood collection.

Nanobiotechnology - Nov 2017

Easy Compound Encapsulation and Nanoparticles with Narrow Size Distribution

  • Pronanosomes for Compound Encapsulation
  • PLGA Nanoparticles: Drug Delivery Carriers
  • Gold, Silver and Alloyed Nanoparticles
  • Transfectosome Transfection Reagent
Nanovex Pronanosomes are ready-to-use formulations used to obtain nanovesicles with characteristics allowing the encapsulation of different compounds in a fast and simple way.

Encapsulation provides compound protection, increased bioavailability, controlled delivery, target delivery, great stability and masking undesired tastes...

Cell Culture, Blood - Nov 2017

Do you require access to blood or bone marrow samples from diseased patients?

Do you require access to blood or bone marrow samples from diseased patients?

AllCells specialises in haematopoietic and immunological cells collected from diseased donors. These include Autoimmune diseases, such as Arthritis, Systemic Lupus Erythematosus (SLE), Psoriasis, Diabetes, and Multiple Sclerosis, as well as Haematological cancers such as AML, CML, MM, and Lymphoma. 

Whole Blood, Plasma, Serum, Urine and Peripheral Blood Mononuclear Cells are available from each disease type and in fresh and frozen formats upon request.

Donor clinical reports are available for each sample. This may include auto antibody analysis, general tests, patient medication history, clinical diagnosis and surgeries. If there are other tests required, please let us know and we can check the feasibility.

Flow Cytometry - Oct 2017

Webinar: Flow Cytometry Sample Analysis Using Infinicyt 2.0

Join our 1-hour free webinars on Infinicyt 2.0

Infinicyt™ is the most advanced software for data integration and multidimensional analysis of flow cytometry files. Version 2.0 is now available, offering new solutions for the analysis of flow cytometry data, and includes direct access to Euroflow™ reference databases.
Join us for a series of free webinars, including:
  • Analysis of MM MRD sample with Infinicyt 2.0
  • Analysis of Primary Immunodeficiencies samples (PIDOT kit) with Infinicyt 2.0
  • Analysis of Acute Leukemia samples (ALOT kit and BCP-ALL-MRD kit) with Infinicyt 2.0
Click here to find out more and to register.

For further information, or to request an Infinicyt™ workshop, please contact techsupport@caltagmedsystems.co.uk.
 
Cytognos, the developers of Infinicyt, also provide the EuroFlow screening kits for Multiple Myeloma Minimal Residual Disease (MM MRD), Primary Immunodeficiency Orientation Tube (PIDOT), Acute Leukaemia Orientation Tube (ALOT), and B-cell precursor Acute Lymphoblastic Leukemia (BCP-ALL-MRD). Contact us for more details on these kits.

France, General News - Oct 2017

Solutions en sciences de la vie

Bienvenue dans le bulletin de Caltag Medsystems. Vous trouverez une sélection de nouveaux produits, d’offres spéciales ainsi que les dernières nouveautés distribuées par Caltag Medsystems.

  • Stabilisation des plaquettes
  • Produits d'analyse à base de plaques pour molécules régulatrices
  • Réactifs pour immunohistochimie, histologie et ELISA
  • Kits ELISA de Cloud Clone: Economisez avec des kits ELISA multi-pack
  • Cellules et tissusCasse-tête

General - Oct 2017

Life Science Solutions

  • ELISA Kits: Over 10,000 Available
  • Pure Exosome Isolation and why it Matters
  • Screening of Antigen-Specific T-Cells is now a Reality!
  • Huge Range of Primary Cells and Tissue
  • Nanobiotechnology: Pronanosomes for Fast, Easy Compound Encapsulation
  • Brainteaser: Secret Phrase

Cord Blood - Oct 2017

Cord Blood Cell Inventory

Below is our current inventory of cord blood cells. AllCells offer umbilical cord stem cells, plasma,  mononuclear cells and endothelial cells. These products are available in fresh, frozen, lysate, cell pellet formats and are available from mixed cords or single cord donor. If you do not see your required product listed, please contact me as it could be produced as a custom product.

France, Cell Culture, Newsletter - Sep 2017

Solutions en sciences de la vie : Cellules humaines non-fœtales, Lignées cellulaires, Service d'approvisionnement pour les tissus, Cellules animales, Cellules primaires et milieux

Bienvenue dans le bulletin de Caltag Medsystems. Vous trouverez une sélection de nouveaux produits, d’offres spéciales ainsi que les dernières nouveautés distribuées par Caltag Medsystems.
  • Cellules humaines non-fœtales disponibles
  • Lignées cellulaires maintenant disponibles
  • Service d'approvisionnement pour les tissus
  • Cellules animales
  • Cellules primaires et milieux de haute qualité 


France, Blood - Sep 2017

Produits de leucaphérèse : cellules mononucléaires et plasma riche en plaquettes

Savez-vous que AllCells fournit des produits de leucaphérèse pour accélérer votre recherche ?

Produits de leucaphérèse fournis par AllCells : 
  • LeukoPaks : Cellules mononucléaires et Plasma
  • LeukoPaks mobilisés : Idéal pour l’isolement à grande échelle de cellules CD34+
  • Options pour l'isolement de cellules T


Europe, Blood - Sep 2017

Leukapheresis Packs: Mononuclear Cells and Platelet Rich Plasma

Did you know that AllCells can provide leukopheresis products for your research? 

Leukapheresis Packs available from AllCells:
  • LeukoPaks: Mononuclear Cells and Plasma
  • Mobilised LeukoPaks: Ideal for large scale CD34+ Isolations
  • T Cell Isolation Options


Cell Culture - Aug 2017

Primary Cell Inventory from DV Biologics

Hello, 

Having trouble sourcing a particular cell type? Let us do the work for you! 

DV Biologics offers a diverse range of highly characterised, difficult to source, human cell types and tissue-derived products. Click here to see the current inventory.

France, General News - Aug 2017

Solutions en sciences de la vie : Kits ELISA, Echantillons protéomique, recherche en neurologie, qPCR, anticorps, cellules et tissus

Bienvenue dans le bulletin de Caltag Medsystems.  Vous trouverez une sélection de nouveaux produits, d’offres spéciales ainsi que les dernières nouveautés distribuées par Caltag Medsystems.Kits 

  • ELISA de Cloud Clone: Economisez avec des kits ELISA multi-pack 
  • Kits de préparation d'échantillons protéomiques optimisés
  • World Brain Day- Réactifs pour la recherche en neurologie
  • 40 cycles de qPCR en moins de 10 minutes  
  • Des anticorps pour les cibles de points de contrôle immunitaire
  • Cellules et tissus


Cell Culture - Aug 2017

Human & Animal Skin and Skin Cells


Caltag Medsystems supply both fresh and frozen human skin for use in different applications including: drug permeation, skin irritation, toxicology, and metabolism, as well as primary cell isolation.

In addition, we provide a wide range of skin cells: fibroblasts, keratinocytes, melanocytes and skin subcellular fractions

Skin Samples - Options Available
Fresh Skin
Frozen Skin
Animal Skin
Primary Skin Cells
Skin Subcellular Fractions

Adipogen - Aug 2017

Immune Regulation and Fusion Proteins


Good morning,

My name is Adedayo Leduwe, a Product Manager at Caltag Medsystems, the UK distributor for Chimerigen - experts in high quality fusion protein and specialists in the production of unique immunoglobulin based chimeric fusion proteins. These reagents are used successfully in basic and applied research, providing advanced Immune Checkpoint Proteins, Interleukins and other Cytokines. Please click here and request your brochure summarising the Immune Regulation Proteins we have to offer.

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