20S Immunoproteasome Assay Kit

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South Bay Bio
Product Code: SBB-KP0037
Supplier: South Bay Bio
CodeSizePrice
SBB-KP0037-KI011 Kit£717.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Human
Application: Compound Screening
Shipping:
DRY ICE
Storage:
-20°C

Images

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% Signal to Background of Continuous Real-Time TR-FRET Parkin titration (autoubiquitination): Serial dilutions of Parkin W403A from 50nM to 3.125nM and 300nM wt Parkin were mixed with UBA1, UBE2D3 and trf-Ub (pS65) mix. Reactions were initiated wit
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% Signal to Background of Continuous Real-Time TR-FRET Parkin titration (autoubiquitination): Serial dilutions of Parkin W403A from 50nM to 3.125nM and 300nM wt Parkin were mixed with UBA1, UBE2D3 and trf-Ub (pS65) mix. Reactions were initiated wit

Documents

Further Information

Detection Type:
Fluorescent
EClass:
32160000
Handling Advice:
Aliquot to avoid freeze/thaw cycles.Avoid freeze/thaw cycles.
Kit Contains:
10x 20S Immunoproteasome | 50x LLVY-AMC, Chemotrypsin-like activity | 50x PAL-AMC, beta1i/PSMB9 specific substrate | 50x ANW-AMC, beta5i/PSMB8 specific substrate | 50x Inhibitor (ONX-0914, 2mM) in 100% DMSO | 10x Reaction Buffer | 50x SDS(1.75%) in H20 | 100x free AMC Standard (40µM)
Long Description:
South Bay Bio's kit is designed to test for specific activity of 20S immunoproteasome. The kit provides purified 20S immunoproteasome and is designed to test for Chymotrypsin-like activity (Suc-LLVY-AMC) and Caspase-like activity of the immunoproteasome subunits beta1i/ PSMB9 (PAL-AMC) and beta5i/PSMB8 (ANW-AMC). Additionally, the compound, ONX-0914, which can be used to inhibit specifically the subunit beta5i/LMP7 20S immunoproteasome is included. All peptide substrates are conjugated to AMC, which upon proteasome catalyzed hydrolyses display fluorescence at Excitation=345nm, Emission= 445nm; allowing for a real-time read out of 20S immunoproteasome specific activity.
Package Type:
Vial
Product Description:
South Bay Bio's kit is designed to test for specific activity of 20S immunoproteasome. The kit provides purified 20S immunoproteasome and is designed to test for Chymotrypsin-like activity (Suc-LLVY-AMC) and Caspase-like activity of the immunoproteasome subunits beta1i/ PSMB9 (PAL-AMC) and beta5i/PSMB8 (ANW-AMC). Additionally, the compound, ONX-0914, which can be used to inhibit specifically the subunit beta5i/LMP7 20S immunoproteasome is included. All peptide substrates are conjugated to AMC, which upon proteasome catalyzed hydrolyses display fluorescence at Excitation=345nm, Emission= 445nm; allowing for a real-time read out of 20S immunoproteasome specific activity.
Specificity:
Readout: Endpoint/Kinetic Label or Dye: 7-Amino-4-methylcoumarin Detection Method: Fluorescence Substrate Properties: Protein-based Substrate. Excitation/Emission (nm): 345/445
Transportation:
Non-hazardous
UNSPSC Category:
Chemistry test kits or supplies
UNSPSC Number:
41116104