Mouse IgG1 Negative Control

Consult the appropriate fact sheet to determine the amount of antibodies to be used as a control for PBMC or Whole Blood. PBMC: Add 10 ?l of MAB/10^6 PBMC in100 ?l PBS. Mix gently and incubate for 15 minutes at 2? to 8?C. Wash twice with PBS and analyze. WHOLE BLOOD: Add10 ?l of MAB/100 ?l of Whole Blood. Mix gently and incubate for 15 minutes at room temperature 20?C. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturer?s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at ?633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Excites at 488nm and emits at 670nm. Store protected from light. Optimal concentration should be evaluated by serial dilutions.

This monoclonal antibody to IgG1 is a negative control for Immunofluorescence staining with Pure, FITC, Biotin, PE and APC mouse monoclonal antibodies of the IgG1 subclass. It is used to monitor the level of non-specific binding of mouse monoclonal antibodies to human cell surface antigens.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Titered for flow cytometry

Flow Cytometry

Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Flow Cytometry Staining

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Unconjugated

Reagents are stable for the period shown on the vial label when stored properly

Protein A/G Chromatography

IgG1

P07750