(+)-Biotin N-hydroxysuccinimide ester

Chemodex
Product Code: CDX-B0145
Supplier: Chemodex

CodeSizePrice
CDX-B0145-M100100 mg£70.00
Quantity:
CDX-B0145-M250250 mg£145.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Antibody Isotype: n/a
Antibody Clone: n/a
Regulatory Status: RUO
Shipping:
Ambient
Storage:
-20°C

Images

1 / 1
Chemical Structure

Chemical Structure

Further Information

Alternate Names/Synonyms:
(+)-Biotin N-succinimidyl ester; d-Biotin NHS ester; BNHS; Biotinyl-N-hydroxy-succinimide; N-Hydroxysuccinimidobiotin; N-Succinimidyl D-biotinate; NHS-Biotin; Biotin-Osu; NSC 345668
Appearance:
White to off-white powder.
CAS:
35013-72-0
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Protect from light and moisture.
InChi:
InChI=1S/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)/t8-,9-,13-/m0/s1
InChiKey:
YMXHPSHLTSZXKH-RVBZMBCESA-N
Long Description:
Chemical. CAS: 35013-72-0. Formula: C14H19N3O5S. MW: 341.38. Biotin succinimidyl ester is the most popular amine-reactive biotinylating agent for modifying proteins, peptides, antibodies, oligonucleotides, microbeads and other biological molecules. It forms irreversible amide bonds. Typically coupled to primary amine in the pH range 6.5-8.5. This cell-permable activated biotin derivative can be readily coupled to primary or secondary amines of analyte compounds or proteins under mild conditions. The resulting biotinylated derivatives have been widely used in streptavidin or avidin systems and labeling methods, with many applications in immunology and histochemistry. It is used to selectively label Escherichia coli cell envelope proteins in vivo. It preferentially labels outer membrane, periplasmic, and inner membrane proteins as well as a specific inner membrane marker protein (Tet-LacZ).
MDL:
MFCD00078531
Molecular Formula:
C14H19N3O5S
Molecular Weight:
341.38
Package Type:
Vial
Product Description:
Biotin succinimidyl ester is the most popular amine-reactive biotinylating agent for modifying proteins, peptides, antibodies, oligonucleotides, microbeads and other biological molecules. It forms irreversible amide bonds. Typically coupled to primary amine in the pH range 6.5-8.5. This cell-permable activated biotin derivative can be readily coupled to primary or secondary amines of analyte compounds or proteins under mild conditions. The resulting biotinylated derivatives have been widely used in streptavidin or avidin systems and labeling methods, with many applications in immunology and histochemistry. It is used to selectively label Escherichia coli cell envelope proteins in vivo. It preferentially labels outer membrane, periplasmic, and inner membrane proteins as well as a specific inner membrane marker protein (Tet-LacZ).
Purity:
>98% (HPLC)
SMILES:
O=C(ON1C(CCC1=O)=O)CCCC[C@@H]2SC[C@@]3([H])[C@]2([H])NC(N3)=O
Solubility Chemicals:
Soluble in DMSO or DMF.
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.

References

(1) D.M. Boorsma, et al.; J. Microsc. 143, 197 (1986) | (2) P. Kongtawelert & P. Ghosh; Clin. Chem. Acta 195, 17 (1990) | (3) G. Paganelli, et al.; Int. J. Cancer 45, 1184 (1990) | (4) C. Wagener, et al.; Meth. Enzymol. 184, 518 (1990) | (5) B. Baumeister, et al.; Int. J. Pep. Res. Therap. 11, 139 (2005) | (6) G.T. Hermanson; Bioconjugate Techn. 2nd p506-545 (2008)